YJL046W / AIM22

Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL046W / AIM22

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:3.90|P-value:4.30E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:7.82|P-value:1.71E-15 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.32|P-value:4.10E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.81|P-value:6.13E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.10|P-value:8.86E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.58|P-value:1.98E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:4.15|P-value:1.47E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.20|P-value:6.32E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:4.50|P-value:2.92E-6 ID:SGTC_227|Compound:fluconazole|FD-Score:3.77|P-value:7.16E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.30|P-value:1.44E-47 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.33|P-value:3.94E-8 ID:SGTC_279|Compound:1273-0055|FD-Score:3.50|P-value:2.14E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.29|P-value:4.67E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.55|P-value:1.73E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:4.32|P-value:6.87E-6 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.42|P-value:2.81E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:3.27|P-value:5.01E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.17|P-value:1.32E-5 ID:SGTC_654|Compound:1124-0271|FD-Score:3.19|P-value:6.68E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.24|P-value:5.55E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:3.67|P-value:1.08E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.17|P-value:7.06E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.45|P-value:2.51E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:4.61|P-value:1.71E-6 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.48|P-value:2.30E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.86|P-value:5.11E-5 ID:SGTC_1635|Compound:st007035|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.99|P-value:2.42E-7 ID:SGTC_1722|Compound:st034304|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.67|P-value:1.08E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.71|P-value:9.13E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.45|P-value:2.59E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.11|P-value:8.61E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.40|P-value:3.05E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-6.61|P-value:1.36E-11 ID:SGTC_2607|Compound:solasodine|FD-Score:5.37|P-value:3.24E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.46|P-value:2.44E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.18|P-value:6.74E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.21|P-value:6.22E-4 ID:SGTC_2711|Compound:st079103|FD-Score:-3.19|P-value:6.49E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.15|P-value:7.65E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.63|P-value:1.53E-6 ID:SGTC_2878|Compound:9043973|FD-Score:-3.64|P-value:1.22E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.13|P-value:1.55E-5 ID:SGTC_2902|Compound:5787006|FD-Score:-3.56|P-value:1.69E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.35|P-value:5.81E-6 ID:SGTC_2916|Compound:7875993|FD-Score:-3.29|P-value:4.59E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.44|P-value:2.60E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-6.23|P-value:1.73E-10 ID:SGTC_3219|Compound:9131112|FD-Score:-3.32|P-value:4.14E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.31|P-value:4.29E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.90|P-value:4.30E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:7.82|P-value:1.71E-15 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.32|P-value:4.10E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.81|P-value:6.13E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.10|P-value:8.86E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.58|P-value:1.98E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:4.15|P-value:1.47E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.20|P-value:6.32E-4 ID:SGTC_203|Compound:k066-0004|FD-Score:4.50|P-value:2.92E-6 ID:SGTC_227|Compound:fluconazole|FD-Score:3.77|P-value:7.16E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.30|P-value:1.44E-47 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.33|P-value:3.94E-8 ID:SGTC_279|Compound:1273-0055|FD-Score:3.50|P-value:2.14E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.29|P-value:4.67E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.55|P-value:1.73E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:4.32|P-value:6.87E-6 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-3.42|P-value:2.81E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:3.27|P-value:5.01E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.17|P-value:1.32E-5 ID:SGTC_654|Compound:1124-0271|FD-Score:3.19|P-value:6.68E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.24|P-value:5.55E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:3.67|P-value:1.08E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.17|P-value:7.06E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.45|P-value:2.51E-4 ID:SGTC_1223|Compound:0764-0573|FD-Score:4.61|P-value:1.71E-6 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.48|P-value:2.30E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.86|P-value:5.11E-5 ID:SGTC_1635|Compound:st007035|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.99|P-value:2.42E-7 ID:SGTC_1722|Compound:st034304|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.67|P-value:1.08E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.71|P-value:9.13E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.45|P-value:2.59E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.11|P-value:8.61E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.40|P-value:3.05E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-6.61|P-value:1.36E-11 ID:SGTC_2607|Compound:solasodine|FD-Score:5.37|P-value:3.24E-8 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.46|P-value:2.44E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.18|P-value:6.74E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.21|P-value:6.22E-4 ID:SGTC_2711|Compound:st079103|FD-Score:-3.19|P-value:6.49E-4 ID:SGTC_2730|Compound:imipramine|FD-Score:3.15|P-value:7.65E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.63|P-value:1.53E-6 ID:SGTC_2878|Compound:9043973|FD-Score:-3.64|P-value:1.22E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.13|P-value:1.55E-5 ID:SGTC_2902|Compound:5787006|FD-Score:-3.56|P-value:1.69E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.35|P-value:5.81E-6 ID:SGTC_2916|Compound:7875993|FD-Score:-3.29|P-value:4.59E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.44|P-value:2.60E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-6.23|P-value:1.73E-10 ID:SGTC_3219|Compound:9131112|FD-Score:-3.32|P-value:4.14E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.31|P-value:4.29E-4

Top fitness defect scores for YJL046W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 14.30 5.3 µM 1.44E-47 110 6.86E-47
2 SGTC_36 0335-0881 7.82 14.2 µM 1.71E-15 ubiquinone biosynthesis & proteasome 37 2.72E-15
3 SGTC_2607 solasodine 5.37 47.4 µM 3.24E-8 33 4.04E-8
4 SGTC_275 trichlorophene 5.33 5.7 µM 3.94E-8 mitochondrial stress 149 4.90E-8
5 SGTC_1223 0764-0573 4.61 147.0 µM 1.71E-6 8 2.01E-6
6 SGTC_116 0155-0182 4.58 31.8 µM 1.98E-6 mitochondrial response to ROS 51 2.33E-6
7 SGTC_203 k066-0004 4.50 30.9 µM 2.92E-6 RSC & ERG11 13 3.41E-6
8 SGTC_386 1509-0011 4.32 3.1 µM 6.87E-6 127 7.94E-6
9 SGTC_625 k213-0058 4.17 121.0 µM 1.32E-5 translation 92 1.51E-5
10 SGTC_134 0269-0018 4.15 6.9 µM 1.47E-5 125 1.68E-5
11 SGTC_34 0469-0683 3.90 81.8 µM 4.30E-5 SWF1 & branched chain AA biosynthesis 31 4.84E-5
12 SGTC_1468 k284-1086 3.86 134.0 µM 5.11E-5 DNA intercalators 42 5.74E-5
13 SGTC_70 0141-0289 3.81 43.3 µM 6.13E-5 mitochondrial stress 187 6.86E-5
14 SGTC_227 fluconazole 3.77 33.5 µM 7.16E-5 azole & statin 96 8.00E-5
15 SGTC_902 1120-0002 3.67 2.1 µM 1.08E-4 azole & statin 56 1.20E-4
16 SGTC_1635 st007035 3.57 42.4 µM 1.60E-4 242 1.77E-4
17 SGTC_163 1327-0036 3.56 7.5 µM 1.69E-4 136 1.86E-4
18 SGTC_279 1273-0055 3.50 2.9 µM 2.14E-4 azole & statin 52 2.35E-4
19 SGTC_1188 1403-0014 3.45 4.6 µM 2.51E-4 mitochondrial stress 77 2.76E-4
20 SGTC_1966 st070145 3.45 16.1 µM 2.59E-4 39 2.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.540 2.59E-253 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.463 1.42E-177 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.461 1.93E-176 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.447 2.63E-164 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.446 2.08E-163 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.445 4.57E-163 YDR079W PET100 hom Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
0.435 2.17E-155 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.430 3.18E-151 YDR237W MRPL7 hom Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.430 5.84E-151 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.429 3.44E-150 YER061C CEM1 hom Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
0.425 2.29E-147 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.425 2.55E-147 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.421 2.12E-144 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.420 4.21E-143 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.418 5.19E-142 YGR220C MRPL9 hom Mitochondrial ribosomal protein of the large subunit