HIPHOP chemogenomics database

YJL062W / LAS21

Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL062W / LAS21

Click on Significant Values for Screen Details

Top fitness defect scores for YJL062W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_3084	9116877	8.70	49.5 µM	4.39E-18		1	1.64E-18
2	SGTC_1921	5740136	7.50	50.0 µM	6.64E-14	TRP & mitochondrial translation	7	3.16E-14
3	SGTC_800	3448-7362	6.85	155.0 µM	6.95E-12	translation	28	3.71E-12
4	SGTC_976	1348-1067	6.76	47.6 µM	1.29E-11	60S ribosome export	3	6.99E-12
5	SGTC_1991	st073000	6.16	8.9 µM	5.99E-10	NEO1	32	3.58E-10
6	SGTC_1076	itraconazole	5.93	331.0 nM	2.45E-9		8	1.52E-9
7	SGTC_3091	9116372	5.76	49.5 µM	6.48E-9		3	4.11E-9
8	SGTC_928	2764-0428	5.37	58.7 µM	5.87E-8	cell wall	23	3.93E-8
9	SGTC_767	0929-0023	5.30	86.0 µM	8.50E-8	TRP & mitochondrial translation	25	5.75E-8
10	SGTC_1862	st057513	4.94	3.8 µM	5.49E-7		2	3.89E-7
11	SGTC_730	1436-0052	4.83	41.2 µM	9.35E-7	mitochondrial processes	3	6.72E-7
12	SGTC_1386	3228-0095	4.83	33.8 µM	9.38E-7		7	6.74E-7
13	SGTC_1594	piperine	4.73	70.1 µM	1.56E-6		5	1.14E-6
14	SGTC_2600	tangeritin	4.56	88.0 µM	3.40E-6		20	2.52E-6
15	SGTC_808	0711-0032	4.56	135.0 µM	3.45E-6		5	2.56E-6
16	SGTC_723	4182-0001	4.47	115.0 µM	5.28E-6		19	3.96E-6
17	SGTC_43	0570-0105	4.43	72.5 µM	6.20E-6	ERG2	76	4.67E-6
18	SGTC_227	fluconazole	4.39	33.5 µM	7.55E-6	azole & statin	68	5.72E-6
19	SGTC_1613	st002381	4.38	52.1 µM	7.75E-6		15	5.87E-6
20	SGTC_3131	9127911	4.36	49.5 µM	8.68E-6		18	6.59E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.105	1.02E-9	YBR238C	YBR238C	hom	Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
0.100	5.65E-9	YGL008C	PMA1	het	Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.092	8.92E-8	YJR145C	RPS4A	hom	Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.088	3.52E-7	YGL007W_d	BRP1_d	hom	Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
0.084	1.07E-6	YGL250W	RMR1	hom	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.082	1.97E-6	YGR118W	RPS23A	hom	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.082	2.16E-6	YML128C	MSC1	hom	Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
0.078	6.34E-6	YDR525W_d	API2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.078	6.43E-6	YEL065W	SIT1	hom	Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
0.078	6.56E-6	YJL069C	UTP18	het	Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.075	1.21E-5	YOR006C	TSR3	hom	Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.074	1.67E-5	YGL012W	ERG4	hom	C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.073	2.60E-5	YNR063W_p	YNR063W_p	hom	Putative zinc-cluster protein of unknown function
0.072	3.21E-5	YNL100W	AIM37	hom	Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.071	3.94E-5	YDR372C	VPS74	hom	Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33