HIPHOP chemogenomics database

YJL073W / JEM1

DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL073W / JEM1

Click on Significant Values for Screen Details

Top fitness defect scores for YJL073W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_138	0850-0259	17.10	40.9 µM	1.61E-62	cell wall	11	9.44E-66
2	SGTC_2584	androsterone	9.86	48.7 µM	3.95E-22	cell wall	17	3.22E-23
3	SGTC_514	4466-0038	8.35	2.6 nM	2.14E-16	cell wall	33	3.50E-17
4	SGTC_951	1171-0579	8.24	82.7 µM	5.17E-16	cell wall	13	8.84E-17
5	SGTC_690	glyoxal	8.17	20.3 µM	8.47E-16		27	1.49E-16
6	SGTC_304	1310-0298	6.86	97.0 µM	1.21E-11	cell wall	11	3.51E-12
7	SGTC_2865	9030313	6.79	52.0 µM	1.91E-11		2	5.67E-12
8	SGTC_2049	5262831	6.55	67.2 µM	8.66E-11		3	2.79E-11
9	SGTC_2924	9010168	5.97	62.8 µM	3.02E-9	cell wall signaling	9	1.17E-9
10	SGTC_1132	3456-3614	5.12	2.5 µM	3.10E-7	tubulin folding & SWR complex	15	1.53E-7
11	SGTC_863	0466-0191	5.06	129.0 µM	4.21E-7	cell wall	44	2.11E-7
12	SGTC_2845	9003024	5.06	43.0 µM	4.26E-7	amide catabolism	24	2.14E-7
13	SGTC_2844	9010103	5.06	26.0 µM	4.28E-7		8	2.15E-7
14	SGTC_1215	3852-0577	5.00	11.2 µM	5.64E-7		7	2.88E-7
15	SGTC_3131	9127911	4.94	49.5 µM	7.63E-7		9	3.95E-7
16	SGTC_672	1441-0018	4.88	53.7 µM	9.97E-7		4	5.24E-7
17	SGTC_883	0850-0245	4.83	24.6 µM	1.30E-6	cell wall	15	6.91E-7
18	SGTC_2965	9082772	4.82	38.6 µM	1.36E-6	mitochondrial response to ROS	23	7.25E-7
19	SGTC_3074	9116018	4.80	49.5 µM	1.49E-6	RNA processing & uracil transport	9	8.00E-7
20	SGTC_1606	st000094	4.67	49.2 µM	2.72E-6	60S ribosome export	6	1.51E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.378	5.64E-114	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.374	1.09E-111	YOL003C	PFA4	hom	Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.373	3.24E-111	YJR142W_p	YJR142W_p	hom	Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.343	4.07E-93	YPL115C	BEM3	hom	Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.340	1.30E-91	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.338	3.45E-90	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.336	2.13E-89	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.333	1.63E-87	YLL032C	YLL032C	hom	Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.325	3.30E-83	YJR111C_p	YJR111C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.318	1.76E-79	YDL066W	IDP1	hom	Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.317	3.43E-79	YJL080C	SCP160	hom	Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.308	2.77E-74	YML117W	NAB6	hom	Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.304	1.10E-72	YDL021W	GPM2	hom	Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.303	3.36E-72	YKL076C_d	PSY1_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.295	1.33E-68	YCL005W	LDB16	hom	Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria