YJL125C / GCD14

Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL125C / GCD14

Click on Significant Values for Screen Details ID:SGTC_312|Compound:4092-0653|FD-Score:-2.98|P-value:3.21E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:-3.18|P-value:1.30E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:-3.53|P-value:2.48E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:-2.80|P-value:6.57E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.99|P-value:1.61E-6 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.19|P-value:5.04E-7 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.22|P-value:4.11E-7 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.44|P-value:1.10E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-2.95|P-value:3.54E-4 ID:SGTC_458|Compound:nppb|FD-Score:4.50|P-value:7.55E-8 ID:SGTC_530|Compound:4358-1714|FD-Score:2.88|P-value:3.73E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:2.79|P-value:5.50E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-2.70|P-value:9.96E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:2.72|P-value:7.19E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.50|P-value:2.12E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:2.97|P-value:2.49E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:2.66|P-value:9.12E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.00|P-value:2.23E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.16|P-value:1.45E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.42|P-value:4.21E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-2.98|P-value:3.17E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:2.85|P-value:4.14E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.12|P-value:7.37E-7 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.73|P-value:6.58E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.52|P-value:1.91E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.71|P-value:7.59E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.80|P-value:5.15E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.40|P-value:1.41E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.08|P-value:1.58E-4 ID:SGTC_1522|Compound:st012842|FD-Score:2.87|P-value:3.92E-4 ID:SGTC_1886|Compound:st057356|FD-Score:2.82|P-value:4.73E-4 ID:SGTC_1985|Compound:st071382|FD-Score:2.68|P-value:8.36E-4 ID:SGTC_2782|Compound:7736369|FD-Score:2.75|P-value:6.38E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-2.78|P-value:7.12E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-2.98|P-value:3.21E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:-3.18|P-value:1.30E-4 ID:SGTC_322|Compound:k048-0136|FD-Score:-3.53|P-value:2.48E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:-2.80|P-value:6.57E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.99|P-value:1.61E-6 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.19|P-value:5.04E-7 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.22|P-value:4.11E-7 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.44|P-value:1.10E-7 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-2.95|P-value:3.54E-4 ID:SGTC_458|Compound:nppb|FD-Score:4.50|P-value:7.55E-8 ID:SGTC_530|Compound:4358-1714|FD-Score:2.88|P-value:3.73E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:2.79|P-value:5.50E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-2.70|P-value:9.96E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:2.72|P-value:7.19E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.50|P-value:2.12E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:2.97|P-value:2.49E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:2.66|P-value:9.12E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.00|P-value:2.23E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.16|P-value:1.45E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:-3.42|P-value:4.21E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-2.98|P-value:3.17E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:2.85|P-value:4.14E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.12|P-value:7.37E-7 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.73|P-value:6.58E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.52|P-value:1.91E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:2.71|P-value:7.59E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:2.80|P-value:5.15E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.40|P-value:1.41E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.08|P-value:1.58E-4 ID:SGTC_1522|Compound:st012842|FD-Score:2.87|P-value:3.92E-4 ID:SGTC_1886|Compound:st057356|FD-Score:2.82|P-value:4.73E-4 ID:SGTC_1985|Compound:st071382|FD-Score:2.68|P-value:8.36E-4 ID:SGTC_2782|Compound:7736369|FD-Score:2.75|P-value:6.38E-4 ID:SGTC_2987|Compound:9018338|FD-Score:-2.78|P-value:7.12E-4

Top fitness defect scores for YJL125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_458 nppb 4.50 133.0 µM 7.55E-8 1 3.37E-6 1.33
2 SGTC_454 lyso-paf C-16 4.44 6.7 µM 1.10E-7 3 4.47E-6 0.58
3 SGTC_1445 4239-0156 4.40 97.7 µM 1.41E-7 2 5.39E-6 0.09
4 SGTC_452 diindolylmethane 4.22 8.0 µM 4.11E-7 RNA processing & uracil transport 3 1.20E-5 0.47
5 SGTC_450 aminopyrene 4.19 156.0 nM 5.04E-7 6 1.40E-5 0.05
6 SGTC_1119 parthenolide 4.12 318.5 nM 7.37E-7 TSC3-RPN4 9 1.85E-5 0.38
7 SGTC_446 go6976 3.99 132.0 µM 1.61E-6 2 3.33E-5 0.42
8 SGTC_1203 3992-3522 3.73 103.0 µM 6.58E-6 14 9.57E-5 0.03
9 SGTC_1231 0242-0792 3.52 125.0 µM 1.91E-5 4 2.13E-4 0.17
10 SGTC_734 2023-0417 3.50 80.0 µM 2.12E-5 ergosterol biosynthesis 12 2.30E-4 0.01
11 SGTC_1510 1123-0200 3.08 71.5 µM 1.58E-4 29 0.00104 0.03
12 SGTC_969 1313-0238 3.00 134.0 µM 2.23E-4 mitochondrial processes 43 0.00135 0.00
13 SGTC_763 3453-1109 2.97 128.0 µM 2.49E-4 17 0.00147 0.03
14 SGTC_530 4358-1714 2.88 28.0 µM 3.73E-4 22 0.00199 0.01
15 SGTC_1522 st012842 2.87 45.6 µM 3.92E-4 RNA pol III & RNase P/MRP 28 0.00207 0.02
16 SGTC_1096 rimcazole 2.85 32.2 µM 4.14E-4 9 0.00215 0.01
17 SGTC_1886 st057356 2.82 49.9 µM 4.73E-4 60S ribosome export 22 0.00238 0.24
18 SGTC_1373 2887-0003 2.80 58.5 µM 5.15E-4 Golgi 36 0.00254 0.09
19 SGTC_604 1000-0114 2.79 314.0 µM 5.50E-4 10 0.00267 0.04
20 SGTC_2782 7736369 2.75 71.4 µM 6.38E-4 30 0.00298 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 3.06E-19 YOR154W_p SLP1_p hom Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
0.145 3.42E-17 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.135 3.34E-15 YPL236C ENV7 hom Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
0.118 6.76E-12 YNL046W_p YNL046W_p hom Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.115 2.61E-11 YJL083W TAX4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
0.114 3.75E-11 YMR196W_p YMR196W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
0.113 4.97E-11 YOL043C NTG2 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
0.112 8.61E-11 YBR277C_d YBR277C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
0.109 2.86E-10 YGR172C YIP1 het Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
0.107 4.90E-10 YMR182C RGM1 hom Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
0.106 7.92E-10 YPR106W ISR1 hom Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
0.105 1.10E-9 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.101 5.26E-9 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.099 7.86E-9 YJR056C YJR056C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.096 2.30E-8 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W