HIPHOP chemogenomics database

YJL125C / GCD14

Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain

fixedexpanded
Profile for YJL125C / GCD14

Click on Significant Values for Screen Details

Top fitness defect scores for YJL125C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_458	nppb	4.50	133.0 µM	7.55E-8		1	3.37E-6	1.33
2	SGTC_454	lyso-paf C-16	4.44	6.7 µM	1.10E-7		3	4.47E-6	0.58
3	SGTC_1445	4239-0156	4.40	97.7 µM	1.41E-7		2	5.39E-6	0.09
4	SGTC_452	diindolylmethane	4.22	8.0 µM	4.11E-7	RNA processing & uracil transport	3	1.20E-5	0.47
5	SGTC_450	aminopyrene	4.19	156.0 nM	5.04E-7		6	1.40E-5	0.05
6	SGTC_1119	parthenolide	4.12	318.5 nM	7.37E-7	TSC3-RPN4	9	1.85E-5	0.38
7	SGTC_446	go6976	3.99	132.0 µM	1.61E-6		2	3.33E-5	0.42
8	SGTC_1203	3992-3522	3.73	103.0 µM	6.58E-6		14	9.57E-5	0.03
9	SGTC_1231	0242-0792	3.52	125.0 µM	1.91E-5		4	2.13E-4	0.17
10	SGTC_734	2023-0417	3.50	80.0 µM	2.12E-5	ergosterol biosynthesis	12	2.30E-4	0.01
11	SGTC_1510	1123-0200	3.08	71.5 µM	1.58E-4		29	0.00104	0.03
12	SGTC_969	1313-0238	3.00	134.0 µM	2.23E-4	mitochondrial processes	43	0.00135	0.00
13	SGTC_763	3453-1109	2.97	128.0 µM	2.49E-4		17	0.00147	0.03
14	SGTC_530	4358-1714	2.88	28.0 µM	3.73E-4		22	0.00199	0.01
15	SGTC_1522	st012842	2.87	45.6 µM	3.92E-4	RNA pol III & RNase P/MRP	28	0.00207	0.02
16	SGTC_1096	rimcazole	2.85	32.2 µM	4.14E-4		9	0.00215	0.01
17	SGTC_1886	st057356	2.82	49.9 µM	4.73E-4	60S ribosome export	22	0.00238	0.24
18	SGTC_1373	2887-0003	2.80	58.5 µM	5.15E-4	Golgi	36	0.00254	0.09
19	SGTC_604	1000-0114	2.79	314.0 µM	5.50E-4		10	0.00267	0.04
20	SGTC_2782	7736369	2.75	71.4 µM	6.38E-4		30	0.00298	0.08

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.154	3.06E-19	YOR154W_p	SLP1_p	hom	Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
0.145	3.42E-17	YER091C	MET6	hom	Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.135	3.34E-15	YPL236C	ENV7	hom	Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
0.118	6.76E-12	YNL046W_p	YNL046W_p	hom	Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.115	2.61E-11	YJL083W	TAX4	hom	EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
0.114	3.75E-11	YMR196W_p	YMR196W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
0.113	4.97E-11	YOL043C	NTG2	hom	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
0.112	8.61E-11	YBR277C_d	YBR277C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
0.109	2.86E-10	YGR172C	YIP1	het	Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
0.107	4.90E-10	YMR182C	RGM1	hom	Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
0.106	7.92E-10	YPR106W	ISR1	hom	Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
0.105	1.10E-9	YNL011C_p	YNL011C_p	hom	Putative protein of unknown function; YNL011C is not an essential gene
0.101	5.26E-9	YER046W	SPO73	hom	Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.099	7.86E-9	YJR056C	YJR056C	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.096	2.30E-8	YML057C-A_d	YML057C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W