YJL134W / LCB3

Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL134W / LCB3

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.21|P-value:7.44E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.96|P-value:6.32E-5 ID:SGTC_179|Compound:1273-0063|FD-Score:4.63|P-value:2.47E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:4.67|P-value:2.13E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:4.34|P-value:9.30E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:12.80|P-value:2.08E-36 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.22|P-value:7.23E-4 ID:SGTC_490|Compound:phenamil|FD-Score:4.12|P-value:2.44E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.68|P-value:1.40E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.04|P-value:3.38E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:-4.53|P-value:5.82E-6 ID:SGTC_598|Compound:3937-0236|FD-Score:-3.25|P-value:8.42E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-3.75|P-value:1.44E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:-3.21|P-value:9.61E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.58|P-value:2.70E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:5.36|P-value:6.52E-8 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.68|P-value:1.38E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.49|P-value:6.86E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.42|P-value:4.76E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.84|P-value:1.03E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:-3.28|P-value:7.74E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:5.58|P-value:2.03E-8 ID:SGTC_1781|Compound:st038725|FD-Score:5.89|P-value:3.51E-9 ID:SGTC_1900|Compound:st060200|FD-Score:-3.53|P-value:3.27E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.24|P-value:8.80E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.24|P-value:1.98E-5 ID:SGTC_1995|Compound:st072814|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.31|P-value:5.34E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.27|P-value:1.25E-5 ID:SGTC_2165|Compound:5727197|FD-Score:3.35|P-value:4.61E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-3.37|P-value:5.71E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.37|P-value:8.06E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.26|P-value:6.31E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.80|P-value:1.21E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.67|P-value:1.45E-4 ID:SGTC_3003|Compound:9075612|FD-Score:-3.47|P-value:3.99E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.21|P-value:7.44E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.96|P-value:6.32E-5 ID:SGTC_179|Compound:1273-0063|FD-Score:4.63|P-value:2.47E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:4.67|P-value:2.13E-6 ID:SGTC_366|Compound:0181-0206|FD-Score:4.34|P-value:9.30E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:12.80|P-value:2.08E-36 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.22|P-value:7.23E-4 ID:SGTC_490|Compound:phenamil|FD-Score:4.12|P-value:2.44E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.68|P-value:1.40E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.04|P-value:3.38E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:-4.53|P-value:5.82E-6 ID:SGTC_598|Compound:3937-0236|FD-Score:-3.25|P-value:8.42E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-3.75|P-value:1.44E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:-3.21|P-value:9.61E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-3.58|P-value:2.70E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:5.36|P-value:6.52E-8 ID:SGTC_1438|Compound:4092-1045|FD-Score:3.68|P-value:1.38E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.49|P-value:6.86E-6 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.42|P-value:4.76E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.84|P-value:1.03E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:-3.28|P-value:7.74E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:5.58|P-value:2.03E-8 ID:SGTC_1781|Compound:st038725|FD-Score:5.89|P-value:3.51E-9 ID:SGTC_1900|Compound:st060200|FD-Score:-3.53|P-value:3.27E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.24|P-value:8.80E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.24|P-value:1.98E-5 ID:SGTC_1995|Compound:st072814|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.31|P-value:5.34E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.27|P-value:1.25E-5 ID:SGTC_2165|Compound:5727197|FD-Score:3.35|P-value:4.61E-4 ID:SGTC_2239|Compound:6685329|FD-Score:-3.37|P-value:5.71E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.37|P-value:8.06E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.26|P-value:6.31E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.80|P-value:1.21E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.67|P-value:1.45E-4 ID:SGTC_3003|Compound:9075612|FD-Score:-3.47|P-value:3.99E-4

Top fitness defect scores for YJL134W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_457 grayanotoxin iii 12.80 135.0 µM 2.08E-36 4 8.15E-38
2 SGTC_1781 st038725 5.89 66.3 µM 3.51E-9 NEO1 58 1.97E-9
3 SGTC_1568 digitonin 5.58 820.0 nM 2.03E-8 sphingolipid biosynthesis & PDR1 14 1.22E-8
4 SGTC_1318 1280-3875 5.36 124.0 µM 6.52E-8 5 4.10E-8
5 SGTC_266 formosanin C 4.67 406.0 nM 2.13E-6 sphingolipid biosynthesis & PDR1 52 1.53E-6
6 SGTC_179 1273-0063 4.63 6.8 µM 2.47E-6 14 1.79E-6
7 SGTC_2502 avocadyne acetate 4.37 18.6 µM 8.06E-6 20 6.08E-6
8 SGTC_366 0181-0206 4.34 4.3 µM 9.30E-6 Golgi 30 7.06E-6
9 SGTC_2081 5214970 4.27 32.3 µM 1.25E-5 19 9.57E-6
10 SGTC_490 phenamil 4.12 164.0 µM 2.44E-5 heme requiring 7 1.92E-5
11 SGTC_546 peri-Naphthalide 4.04 147.0 µM 3.38E-5 DNA damage response 17 2.69E-5
12 SGTC_1438 4092-1045 3.68 4.0 µM 1.38E-4 10 1.15E-4
13 SGTC_528 3492-0019 3.68 172.0 µM 1.40E-4 calcium & mitochondrial duress 242 1.17E-4
14 SGTC_2956 9076196 3.67 2.5 µM 1.45E-4 27 1.21E-4
15 SGTC_1995 st072814 3.59 61.1 µM 1.93E-4 19 1.63E-4
16 SGTC_2165 5727197 3.35 32.1 µM 4.61E-4 59 4.01E-4
17 SGTC_2079 5212404 3.31 121.0 µM 5.34E-4 25 4.68E-4
18 SGTC_2524 avocatin b 3.26 10.6 µM 6.31E-4 44 5.56E-4
19 SGTC_481 bay k-8644 3.22 50.0 µM 7.23E-4 52 6.39E-4
20 SGTC_6 0844-0013 3.21 19.8 µM 7.44E-4 172 6.58E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.290 3.60E-66 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.246 2.23E-47 YOR047C STD1 hom Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]
0.204 1.10E-32 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.159 1.79E-20 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.157 6.55E-20 YDR042C_p YDR042C_p hom Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
0.152 9.15E-19 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.151 1.72E-18 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.146 1.68E-17 YOR061W CKA2 hom Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
0.145 3.91E-17 YDR297W SUR2 hom Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
0.142 1.27E-16 YDR062W LCB2 het Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.139 5.64E-16 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.138 1.09E-15 YPL123C RNY1 hom Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
0.136 2.39E-15 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.131 3.03E-14 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.129 6.15E-14 YOR171C LCB4 hom Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes