YJL149W / DAS1

Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL149W / DAS1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:-3.44|P-value:5.90E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:-3.46|P-value:5.46E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.84|P-value:1.46E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.81|P-value:1.64E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.18|P-value:4.01E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.73|P-value:2.17E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-4.02|P-value:7.34E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:-3.49|P-value:4.93E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.63|P-value:3.05E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.44|P-value:5.90E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.28|P-value:9.72E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:3.49|P-value:4.88E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-3.33|P-value:8.39E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.34|P-value:8.05E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:6.00|P-value:7.29E-9 ID:SGTC_520|Compound:3584-0023|FD-Score:5.51|P-value:9.90E-8 ID:SGTC_623|Compound:k072-0108|FD-Score:3.98|P-value:8.49E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:4.95|P-value:1.53E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:4.27|P-value:2.80E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.49|P-value:4.98E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.36|P-value:7.47E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:16.20|P-value:4.56E-53 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.43|P-value:5.96E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.03|P-value:7.19E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:6.13|P-value:3.64E-9 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.41|P-value:6.47E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.91|P-value:1.13E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.60|P-value:7.21E-6 ID:SGTC_1617|Compound:st002906|FD-Score:3.61|P-value:3.27E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.28|P-value:9.83E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.13|P-value:4.92E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-3.48|P-value:5.13E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.98|P-value:8.50E-5 ID:SGTC_2079|Compound:5212404|FD-Score:-4.39|P-value:1.74E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.40|P-value:6.71E-4 ID:SGTC_2159|Compound:5658611|FD-Score:-3.64|P-value:2.99E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.65|P-value:2.87E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.73|P-value:2.15E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.43|P-value:5.96E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.66|P-value:5.53E-6 ID:SGTC_2605|Compound:digitonin|FD-Score:3.40|P-value:6.58E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.34|P-value:8.13E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.52|P-value:4.49E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.20|P-value:3.67E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.89|P-value:1.22E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.86|P-value:1.34E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.39|P-value:6.82E-4 ID:SGTC_2793|Compound:7325131|FD-Score:6.27|P-value:1.62E-9 ID:SGTC_2800|Compound:7887935|FD-Score:4.22|P-value:3.43E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.60|P-value:3.40E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-8.34|P-value:1.70E-15 ID:SGTC_2992|Compound:9074591|FD-Score:-5.09|P-value:7.93E-7 ID:SGTC_3017|Compound:9080681|FD-Score:3.61|P-value:3.32E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.87|P-value:1.29E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.85|P-value:1.39E-4 ID:SGTC_3317|Compound:9138495|FD-Score:3.57|P-value:3.80E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:-3.44|P-value:5.90E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:-3.46|P-value:5.46E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.84|P-value:1.46E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.81|P-value:1.64E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.18|P-value:4.01E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.73|P-value:2.17E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-4.02|P-value:7.34E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:-3.49|P-value:4.93E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.63|P-value:3.05E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.44|P-value:5.90E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.28|P-value:9.72E-4 ID:SGTC_472|Compound:calcifediol|FD-Score:3.49|P-value:4.88E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:-3.33|P-value:8.39E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.34|P-value:8.05E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:6.00|P-value:7.29E-9 ID:SGTC_520|Compound:3584-0023|FD-Score:5.51|P-value:9.90E-8 ID:SGTC_623|Compound:k072-0108|FD-Score:3.98|P-value:8.49E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:4.95|P-value:1.53E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:4.27|P-value:2.80E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.49|P-value:4.98E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.36|P-value:7.47E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:16.20|P-value:4.56E-53 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.43|P-value:5.96E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.03|P-value:7.19E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:6.13|P-value:3.64E-9 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.41|P-value:6.47E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.91|P-value:1.13E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.60|P-value:7.21E-6 ID:SGTC_1617|Compound:st002906|FD-Score:3.61|P-value:3.27E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.28|P-value:9.83E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.13|P-value:4.92E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-3.48|P-value:5.13E-4 ID:SGTC_2069|Compound:5228469|FD-Score:-3.98|P-value:8.50E-5 ID:SGTC_2079|Compound:5212404|FD-Score:-4.39|P-value:1.74E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.40|P-value:6.71E-4 ID:SGTC_2159|Compound:5658611|FD-Score:-3.64|P-value:2.99E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.65|P-value:2.87E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.73|P-value:2.15E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.43|P-value:5.96E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.66|P-value:5.53E-6 ID:SGTC_2605|Compound:digitonin|FD-Score:3.40|P-value:6.58E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.34|P-value:8.13E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.52|P-value:4.49E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.20|P-value:3.67E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.89|P-value:1.22E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.86|P-value:1.34E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.39|P-value:6.82E-4 ID:SGTC_2793|Compound:7325131|FD-Score:6.27|P-value:1.62E-9 ID:SGTC_2800|Compound:7887935|FD-Score:4.22|P-value:3.43E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.60|P-value:3.40E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-8.34|P-value:1.70E-15 ID:SGTC_2992|Compound:9074591|FD-Score:-5.09|P-value:7.93E-7 ID:SGTC_3017|Compound:9080681|FD-Score:3.61|P-value:3.32E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.87|P-value:1.29E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.85|P-value:1.39E-4 ID:SGTC_3317|Compound:9138495|FD-Score:3.57|P-value:3.80E-4

Top fitness defect scores for YJL149W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 16.20 1.8 µM 4.56E-53 2 2.53E-59
2 SGTC_2793 7325131 6.27 81.8 µM 1.62E-9 5 1.79E-10
3 SGTC_1053 3366-3204 6.13 134.0 µM 3.64E-9 cell wall 8 4.42E-10
4 SGTC_519 k048-0011 6.00 62.8 µM 7.29E-9 2 9.62E-10
5 SGTC_520 3584-0023 5.51 682.0 µM 9.90E-8 5 1.78E-8
6 SGTC_638 3970-0837 4.95 4.1 µM 1.53E-6 18 3.81E-7
7 SGTC_2557 derrustone 4.66 83.8 µM 5.53E-6 15 1.60E-6
8 SGTC_1607 st000305 4.60 50.6 µM 7.21E-6 4 2.16E-6
9 SGTC_711 k292-0785 4.27 179.0 µM 2.80E-5 DNA intercalators 16 9.83E-6
10 SGTC_2800 7887935 4.22 81.8 µM 3.43E-5 RPP1 & pyrimidine depletion 25 1.23E-5
11 SGTC_2698 st077697 4.20 67.5 µM 3.67E-5 13 1.33E-5
12 SGTC_91 0417-1751 4.18 58.4 µM 4.01E-5 Golgi 46 1.46E-5
13 SGTC_2038 4100038 4.13 31.0 µM 4.92E-5 plasma membrane duress 48 1.84E-5
14 SGTC_1024 4507-0395 4.03 26.0 µM 7.19E-5 TSC3-RPN4 25 2.81E-5
15 SGTC_623 k072-0108 3.98 16.9 µM 8.49E-5 heme biosynthesis & mitochondrial translocase 19 3.39E-5
16 SGTC_1110 3-hydroxy-3'-methoxyflavone 3.91 1.2 µM 1.13E-4 iron homeostasis 43 4.65E-5
17 SGTC_2706 st078239 3.89 82.5 µM 1.22E-4 12 5.06E-5
18 SGTC_3144 9087057 3.87 49.5 µM 1.29E-4 14 5.38E-5
19 SGTC_2709 st078560 3.86 56.0 µM 1.34E-4 15 5.64E-5
20 SGTC_3312 9133330 3.85 71.4 µM 1.39E-4 6 5.89E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.398 7.47E-128 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.393 1.34E-124 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.293 2.13E-67 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.272 7.38E-58 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.264 1.32E-54 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.262 7.72E-54 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.248 2.83E-48 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.216 8.05E-37 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.215 2.62E-36 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.214 6.07E-36 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.213 1.07E-35 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.204 6.17E-33 YGR048W UFD1 het Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
0.201 5.82E-32 YDL177C_p YDL177C_p hom Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
0.198 6.45E-31 YJR014W TMA22 hom Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
0.193 1.74E-29 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance