YJL164C / TPK1

cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL164C / TPK1

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:3.29|P-value:5.92E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.38|P-value:5.51E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.37|P-value:5.67E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.17|P-value:2.67E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.62|P-value:2.30E-4 ID:SGTC_459|Compound:nigericin|FD-Score:3.57|P-value:2.14E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:14.90|P-value:2.14E-48 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.35|P-value:5.91E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.72|P-value:1.62E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.79|P-value:9.57E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.38|P-value:4.21E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.50|P-value:2.83E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.43|P-value:6.52E-6 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.41|P-value:3.83E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.96|P-value:4.77E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.49|P-value:2.92E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.57|P-value:2.14E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.20|P-value:9.96E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:4.76|P-value:1.44E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.90|P-value:7.85E-5 ID:SGTC_1687|Compound:st020778|FD-Score:6.68|P-value:2.81E-11 ID:SGTC_1712|Compound:st032288|FD-Score:7.91|P-value:4.35E-15 ID:SGTC_1714|Compound:st033236|FD-Score:-4.44|P-value:8.46E-6 ID:SGTC_1717|Compound:st033143|FD-Score:-3.31|P-value:6.88E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.55|P-value:2.36E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.10|P-value:2.76E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.47|P-value:3.13E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.35|P-value:4.84E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.69|P-value:2.02E-6 ID:SGTC_1866|Compound:st057638|FD-Score:-3.60|P-value:2.44E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.27|P-value:1.11E-7 ID:SGTC_1974|Compound:st069448|FD-Score:4.70|P-value:1.87E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.76|P-value:1.07E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-4.52|P-value:5.90E-6 ID:SGTC_2399|Compound:5884133|FD-Score:3.77|P-value:1.01E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.21|P-value:1.72E-5 ID:SGTC_2570|Compound:licareol|FD-Score:5.86|P-value:4.23E-9 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.41|P-value:4.87E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.08|P-value:2.97E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.26|P-value:6.51E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.30|P-value:7.12E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.74|P-value:1.15E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.26|P-value:8.04E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-3.73|P-value:1.52E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.52|P-value:2.60E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.29|P-value:5.92E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:-3.38|P-value:5.51E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.37|P-value:5.67E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.17|P-value:2.67E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.62|P-value:2.30E-4 ID:SGTC_459|Compound:nigericin|FD-Score:3.57|P-value:2.14E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:14.90|P-value:2.14E-48 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.35|P-value:5.91E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.72|P-value:1.62E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:3.79|P-value:9.57E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.38|P-value:4.21E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.50|P-value:2.83E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.43|P-value:6.52E-6 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.41|P-value:3.83E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.96|P-value:4.77E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.49|P-value:2.92E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.57|P-value:2.14E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.20|P-value:9.96E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:4.76|P-value:1.44E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.90|P-value:7.85E-5 ID:SGTC_1687|Compound:st020778|FD-Score:6.68|P-value:2.81E-11 ID:SGTC_1712|Compound:st032288|FD-Score:7.91|P-value:4.35E-15 ID:SGTC_1714|Compound:st033236|FD-Score:-4.44|P-value:8.46E-6 ID:SGTC_1717|Compound:st033143|FD-Score:-3.31|P-value:6.88E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.55|P-value:2.36E-4 ID:SGTC_1786|Compound:berberine|FD-Score:4.10|P-value:2.76E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.47|P-value:3.13E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.35|P-value:4.84E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.69|P-value:2.02E-6 ID:SGTC_1866|Compound:st057638|FD-Score:-3.60|P-value:2.44E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.27|P-value:1.11E-7 ID:SGTC_1974|Compound:st069448|FD-Score:4.70|P-value:1.87E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.76|P-value:1.07E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-4.52|P-value:5.90E-6 ID:SGTC_2399|Compound:5884133|FD-Score:3.77|P-value:1.01E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.21|P-value:1.72E-5 ID:SGTC_2570|Compound:licareol|FD-Score:5.86|P-value:4.23E-9 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.41|P-value:4.87E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.08|P-value:2.97E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.26|P-value:6.51E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.30|P-value:7.12E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.74|P-value:1.15E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.26|P-value:8.04E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-3.73|P-value:1.52E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.52|P-value:2.60E-4

Top fitness defect scores for YJL164C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_528 3492-0019 14.90 172.0 µM 2.14E-48 calcium & mitochondrial duress 15 1.97E-50
2 SGTC_1712 st032288 7.91 53.4 µM 4.35E-15 calcium & mitochondrial duress 47 1.29E-15
3 SGTC_1687 st020778 6.68 55.4 µM 2.81E-11 93 1.21E-11
4 SGTC_2570 licareol 5.86 100.0 µM 4.23E-9 5 2.26E-9
5 SGTC_1877 wortmannin 5.27 25.0 µM 1.11E-7 59 6.80E-8
6 SGTC_1568 digitonin 4.76 820.0 nM 1.44E-6 sphingolipid biosynthesis & PDR1 20 9.77E-7
7 SGTC_1974 st069448 4.70 26.9 µM 1.87E-6 14 1.28E-6
8 SGTC_1844 rapamycin 4.69 4.0 nM 2.02E-6 123 1.39E-6
9 SGTC_1001 4048-4355 4.43 44.4 µM 6.52E-6 copper-dependent oxidative stress 24 4.69E-6
10 SGTC_2530 3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 4.21 92.6 µM 1.72E-5 22 1.29E-5
11 SGTC_1786 berberine 4.10 21.6 µM 2.76E-5 60S ribosome export 28 2.11E-5
12 SGTC_2575 plumbagin 4.08 510.0 nM 2.97E-5 superoxide 35 2.27E-5
13 SGTC_1179 1021-1431 3.96 9.3 µM 4.77E-5 42 3.71E-5
14 SGTC_922 1866-0035 3.79 480.0 µM 9.57E-5 excess fatty acid 68 7.65E-5
15 SGTC_2399 5884133 3.77 200.0 µM 1.01E-4 cell wall signaling 80 8.09E-5
16 SGTC_2249 haloperidol 3.76 116.4 µM 1.07E-4 ergosterol depletion effects on membrane 129 8.62E-5
17 SGTC_2713 st079149 3.74 53.4 µM 1.15E-4 28 9.27E-5
18 SGTC_459 nigericin 3.57 15.3 µM 2.14E-4 76 1.76E-4
19 SGTC_1322 1348-1070 3.57 12.0 µM 2.14E-4 14 1.76E-4
20 SGTC_1733 st037357 3.55 54.9 µM 2.36E-4 60S ribosome export 49 1.95E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.483 3.89E-196 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.332 2.42E-87 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.320 1.60E-80 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.314 8.33E-78 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.283 1.14E-62 YDL023C_d YDL023C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance
0.272 6.15E-58 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.270 3.84E-57 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.269 1.96E-56 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.265 3.18E-55 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.263 2.80E-54 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.253 3.00E-50 YLR204W QRI5 hom Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
0.240 3.98E-45 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.239 6.81E-45 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.239 1.13E-44 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.231 5.47E-42 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication