HIPHOP chemogenomics database

YJL180C / ATP12

Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL180C / ATP12

Click on Significant Values for Screen Details

Top fitness defect scores for YJL180C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	18.40	5.3 µM	3.91E-69		66	1.51E-75
2	SGTC_1635	st007035	17.10	42.4 µM	2.41E-60		27	6.66E-66
3	SGTC_275	trichlorophene	12.00	5.7 µM	1.02E-30	mitochondrial stress	20	2.03E-33
4	SGTC_908	3448-7491	11.90	47.7 µM	1.64E-30	mitochondrial stress	3	3.42E-33
5	SGTC_2011	4012611	8.62	177.0 µM	8.10E-17	mitochondrial processes	2	3.40E-18
6	SGTC_514	4466-0038	8.11	2.6 nM	4.13E-15	cell wall	37	2.51E-16
7	SGTC_2326	6164455	7.90	177.6 µM	1.97E-14	mitochondrial processes	1	1.39E-15
8	SGTC_386	1509-0011	7.30	3.1 µM	1.37E-12		75	1.43E-13
9	SGTC_995	3931-2021	6.99	34.6 µM	1.08E-11	RPP1 & pyrimidine depletion	5	1.37E-12
10	SGTC_1883	5-benzylidenerhodanine	6.06	20.0 µM	3.13E-9	TRP & mitochondrial translation	19	6.70E-10
11	SGTC_2310	7668677	5.96	200.0 µM	5.70E-9		5	1.29E-9
12	SGTC_576	1073-0113	5.94	22.7 µM	6.23E-9	redox potentiating	19	1.42E-9
13	SGTC_310	1273-0058	5.66	1.1 µM	2.88E-8	mitochondrial stress	153	7.57E-9
14	SGTC_744	1594-0068	5.61	283.0 µM	3.76E-8	amide catabolism	5	1.01E-8
15	SGTC_6	0844-0013	5.00	19.8 µM	8.23E-7		35	2.92E-7
16	SGTC_1353	1498-1206	4.98	8.1 µM	8.97E-7	mitochondrial response to ROS	29	3.21E-7
17	SGTC_607	4491-0986	4.85	66.8 µM	1.63E-6	heme requiring	12	6.15E-7
18	SGTC_880	0833-0487	4.79	87.2 µM	2.17E-6	mitochondrial processes	6	8.40E-7
19	SGTC_2619	obliquin	4.77	87.9 µM	2.38E-6	cell wall	104	9.31E-7
20	SGTC_2370	9071495	4.74	200.0 µM	2.72E-6	mitochondrial processes	12	1.08E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.535	4.62E-248	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.530	5.34E-242	YDR518W	EUG1	hom	Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.516	3.17E-228	YJL023C	PET130	hom	Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.516	1.39E-227	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.508	5.71E-220	YNL213C	RRG9	hom	Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.507	2.14E-218	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.505	4.92E-216	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.503	4.54E-215	YGR220C	MRPL9	hom	Mitochondrial ribosomal protein of the large subunit
0.500	1.57E-211	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.487	7.72E-200	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.483	5.28E-196	YKL194C	MST1	hom	Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
0.481	4.78E-194	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.480	2.05E-193	YNL252C	MRPL17	hom	Mitochondrial ribosomal protein of the large subunit
0.459	2.95E-174	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.457	3.40E-173	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130