YJL217W / REE1

Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL217W / REE1

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:5.61|P-value:6.88E-9 ID:SGTC_89|Compound:0302-0250|FD-Score:3.53|P-value:1.83E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:3.17|P-value:6.91E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.12|P-value:8.23E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:7.61|P-value:6.54E-15 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.34|P-value:3.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.81|P-value:5.99E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.10|P-value:8.77E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.58|P-value:1.53E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.28|P-value:4.66E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.62|P-value:1.29E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.06|P-value:2.03E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.06|P-value:9.15E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.52|P-value:1.92E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.10|P-value:7.86E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.42|P-value:2.83E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.09|P-value:9.24E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.53|P-value:1.83E-4 ID:SGTC_1685|Compound:st024049|FD-Score:3.53|P-value:1.86E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.03|P-value:2.30E-5 ID:SGTC_2008|Compound:4012253|FD-Score:3.27|P-value:4.88E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.04|P-value:9.71E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.45|P-value:2.53E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-6.67|P-value:5.90E-12 ID:SGTC_2378|Compound:9074213|FD-Score:4.06|P-value:2.10E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.45|P-value:2.48E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.23|P-value:5.67E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.12|P-value:7.43E-4 ID:SGTC_2456|Compound:5770359|FD-Score:4.13|P-value:1.52E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.32|P-value:3.96E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.13|P-value:7.94E-4 ID:SGTC_2801|Compound:7907800|FD-Score:6.78|P-value:3.49E-12 ID:SGTC_2832|Compound:7992762|FD-Score:-4.78|P-value:5.57E-7 ID:SGTC_2834|Compound:9001755|FD-Score:6.86|P-value:1.93E-12 ID:SGTC_2844|Compound:9010103|FD-Score:-3.42|P-value:2.45E-4 ID:SGTC_2893|Compound:9048488|FD-Score:3.79|P-value:6.50E-5 ID:SGTC_3124|Compound:9127567|FD-Score:3.17|P-value:6.96E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.08|P-value:9.55E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.12|P-value:8.30E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:5.61|P-value:6.88E-9 ID:SGTC_89|Compound:0302-0250|FD-Score:3.53|P-value:1.83E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:3.17|P-value:6.91E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.12|P-value:8.23E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:7.61|P-value:6.54E-15 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.34|P-value:3.70E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.81|P-value:5.99E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:3.10|P-value:8.77E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.58|P-value:1.53E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.28|P-value:4.66E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.62|P-value:1.29E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.43|P-value:2.65E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.06|P-value:2.03E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.06|P-value:9.15E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.52|P-value:1.92E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.10|P-value:7.86E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.42|P-value:2.83E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.09|P-value:9.24E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.53|P-value:1.83E-4 ID:SGTC_1685|Compound:st024049|FD-Score:3.53|P-value:1.86E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.03|P-value:2.30E-5 ID:SGTC_2008|Compound:4012253|FD-Score:3.27|P-value:4.88E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-3.04|P-value:9.71E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.45|P-value:2.53E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-6.67|P-value:5.90E-12 ID:SGTC_2378|Compound:9074213|FD-Score:4.06|P-value:2.10E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.45|P-value:2.48E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.23|P-value:5.67E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.12|P-value:7.43E-4 ID:SGTC_2456|Compound:5770359|FD-Score:4.13|P-value:1.52E-5 ID:SGTC_2469|Compound:5655019|FD-Score:3.32|P-value:3.96E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.13|P-value:7.94E-4 ID:SGTC_2801|Compound:7907800|FD-Score:6.78|P-value:3.49E-12 ID:SGTC_2832|Compound:7992762|FD-Score:-4.78|P-value:5.57E-7 ID:SGTC_2834|Compound:9001755|FD-Score:6.86|P-value:1.93E-12 ID:SGTC_2844|Compound:9010103|FD-Score:-3.42|P-value:2.45E-4 ID:SGTC_2893|Compound:9048488|FD-Score:3.79|P-value:6.50E-5 ID:SGTC_3124|Compound:9127567|FD-Score:3.17|P-value:6.96E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.08|P-value:9.55E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.12|P-value:8.30E-4

Top fitness defect scores for YJL217W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_429 1112-0049 7.61 25.5 µM 6.54E-15 DNA damage response 7 1.34E-14
2 SGTC_2834 9001755 6.86 58.4 µM 1.93E-12 6 3.42E-12
3 SGTC_2801 7907800 6.78 81.8 µM 3.49E-12 5 6.09E-12
4 SGTC_69 0096-0274 5.61 206.7 µM 6.88E-9 mitochondrial processes 11 9.95E-9
5 SGTC_2456 5770359 4.13 74.5 µM 1.52E-5 14 1.82E-5
6 SGTC_1335 1486-0900 4.06 202.0 µM 2.03E-5 22 2.42E-5
7 SGTC_2378 9074213 4.06 121.4 µM 2.10E-5 RSC complex & mRNA processing 17 2.50E-5
8 SGTC_1994 st072947 4.03 40.9 µM 2.30E-5 14 2.73E-5
9 SGTC_519 k048-0011 3.81 62.8 µM 5.99E-5 27 6.97E-5
10 SGTC_2893 9048488 3.79 26.0 µM 6.50E-5 fatty acid desaturase (OLE1) 25 7.55E-5
11 SGTC_1172 0370-0043 3.62 85.0 µM 1.29E-4 33 1.48E-4
12 SGTC_907 3448-6986 3.58 75.5 µM 1.53E-4 39 1.74E-4
13 SGTC_89 0302-0250 3.53 142.1 µM 1.83E-4 36 2.08E-4
14 SGTC_1642 st009582 3.53 77.7 µM 1.83E-4 RPP1 & pyrimidine depletion 26 2.08E-4
15 SGTC_1685 st024049 3.53 53.1 µM 1.86E-4 TSC3-RPN4 23 2.12E-4
16 SGTC_1374 2889-5571 3.52 141.0 µM 1.92E-4 RSC complex & mRNA processing 59 2.17E-4
17 SGTC_2380 9035439 3.45 200.0 µM 2.48E-4 endomembrane recycling 45 2.79E-4
18 SGTC_2316 5844804 3.45 197.4 µM 2.53E-4 59 2.85E-4
19 SGTC_1185 1319-0113 3.43 81.3 µM 2.65E-4 DNA damage response 23 2.99E-4
20 SGTC_1545 lovastatin 3.42 49.4 µM 2.83E-4 49 3.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.70E-12 YGL044C RNA15 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.116 1.46E-11 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.114 3.22E-11 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.108 3.97E-10 YPR083W MDM36 hom Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
0.104 1.65E-9 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.100 7.01E-9 YDR141C DOP1 het Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
0.098 1.24E-8 YOR227W HER1 hom Protein of unknown function required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
0.097 2.10E-8 YJL034W KAR2 het ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
0.094 5.01E-8 YOR307C SLY41 hom Protein involved in ER-to-Golgi transport
0.094 5.74E-8 YAL020C ATS1 hom Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
0.091 1.13E-7 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.088 3.18E-7 YOL090W MSH2 hom Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
0.087 4.57E-7 YBL071C_d YBL071C_d hom Dubious open reading frame, predicted protein contains a peroxisomal targeting signal
0.082 2.28E-6 YLR098C CHA4 hom DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
0.081 2.43E-6 YNL334C SNO2 hom Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin