YJR008W / MHO1

Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR008W / MHO1

Click on Significant Values for Screen Details ID:SGTC_79|Compound:0052-0007|FD-Score:3.63|P-value:8.43E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:4.14|P-value:8.95E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.67|P-value:6.22E-7 ID:SGTC_169|Compound:k015-0017|FD-Score:3.15|P-value:5.57E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.74|P-value:5.28E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:4.27|P-value:4.80E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:3.16|P-value:5.22E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:5.54|P-value:4.60E-9 ID:SGTC_794|Compound:k038-0031|FD-Score:4.30|P-value:4.14E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.72|P-value:4.83E-7 ID:SGTC_918|Compound:glabranin|FD-Score:3.70|P-value:6.28E-5 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.07|P-value:1.21E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.20|P-value:4.47E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.97|P-value:1.94E-5 ID:SGTC_1675|Compound:st018485|FD-Score:3.05|P-value:7.81E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-4.11|P-value:9.97E-6 ID:SGTC_1901|Compound:st060216|FD-Score:3.14|P-value:5.67E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.27|P-value:3.54E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.93|P-value:2.22E-5 ID:SGTC_2284|Compound:7945075|FD-Score:-3.34|P-value:2.59E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.07|P-value:7.20E-4 ID:SGTC_2421|Compound:5690543|FD-Score:4.55|P-value:1.21E-6 ID:SGTC_2429|Compound:5902844|FD-Score:8.37|P-value:1.92E-18 ID:SGTC_2430|Compound:5571206|FD-Score:-3.38|P-value:2.26E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-4.85|P-value:2.43E-7 ID:SGTC_2464|Compound:5717415|FD-Score:3.72|P-value:5.66E-5 ID:SGTC_2483|Compound:5729050|FD-Score:3.33|P-value:2.76E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.06|P-value:7.63E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.30|P-value:3.07E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.36|P-value:2.46E-4 ID:SGTC_2695|Compound:st077686|FD-Score:4.67|P-value:6.43E-7 ID:SGTC_2739|Compound:tacrine|FD-Score:3.09|P-value:6.87E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.07|P-value:7.28E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.07|P-value:7.25E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.69|P-value:6.42E-5 ID:SGTC_2949|Compound:9072542|FD-Score:3.29|P-value:3.23E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.26|P-value:3.57E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.15|P-value:5.46E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.19|P-value:4.74E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.00|P-value:9.29E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.63|P-value:8.43E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:4.14|P-value:8.95E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.67|P-value:6.22E-7 ID:SGTC_169|Compound:k015-0017|FD-Score:3.15|P-value:5.57E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:-3.74|P-value:5.28E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:4.27|P-value:4.80E-6 ID:SGTC_336|Compound:4111-0029|FD-Score:3.16|P-value:5.22E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:5.54|P-value:4.60E-9 ID:SGTC_794|Compound:k038-0031|FD-Score:4.30|P-value:4.14E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.72|P-value:4.83E-7 ID:SGTC_918|Compound:glabranin|FD-Score:3.70|P-value:6.28E-5 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.07|P-value:1.21E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.20|P-value:4.47E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.97|P-value:1.94E-5 ID:SGTC_1675|Compound:st018485|FD-Score:3.05|P-value:7.81E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-4.11|P-value:9.97E-6 ID:SGTC_1901|Compound:st060216|FD-Score:3.14|P-value:5.67E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.27|P-value:3.54E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.93|P-value:2.22E-5 ID:SGTC_2284|Compound:7945075|FD-Score:-3.34|P-value:2.59E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.07|P-value:7.20E-4 ID:SGTC_2421|Compound:5690543|FD-Score:4.55|P-value:1.21E-6 ID:SGTC_2429|Compound:5902844|FD-Score:8.37|P-value:1.92E-18 ID:SGTC_2430|Compound:5571206|FD-Score:-3.38|P-value:2.26E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-4.85|P-value:2.43E-7 ID:SGTC_2464|Compound:5717415|FD-Score:3.72|P-value:5.66E-5 ID:SGTC_2483|Compound:5729050|FD-Score:3.33|P-value:2.76E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.06|P-value:7.63E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.30|P-value:3.07E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.36|P-value:2.46E-4 ID:SGTC_2695|Compound:st077686|FD-Score:4.67|P-value:6.43E-7 ID:SGTC_2739|Compound:tacrine|FD-Score:3.09|P-value:6.87E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.07|P-value:7.28E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.07|P-value:7.25E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.22|P-value:4.25E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.69|P-value:6.42E-5 ID:SGTC_2949|Compound:9072542|FD-Score:3.29|P-value:3.23E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-3.26|P-value:3.57E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.15|P-value:5.46E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.19|P-value:4.74E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.00|P-value:9.29E-4

Top fitness defect scores for YJR008W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2429 5902844 8.37 64.4 µM 1.92E-18 2 2.80E-17
2 SGTC_372 0155-0182 5.54 15.9 µM 4.60E-9 mitochondrial response to ROS 7 1.51E-8
3 SGTC_915 methyl methanesulfonate (MMS) 4.72 110.0 mM 4.83E-7 DNA damage response 21 1.15E-6
4 SGTC_2695 st077686 4.67 52.0 µM 6.43E-7 19 1.51E-6
5 SGTC_2421 5690543 4.55 90.7 µM 1.21E-6 11 2.72E-6
6 SGTC_794 k038-0031 4.30 316.0 µM 4.14E-6 ubiquinone biosynthesis & proteasome 98 8.55E-6
7 SGTC_333 3486-0131 4.27 40.1 µM 4.80E-6 amide catabolism 8 9.82E-6
8 SGTC_101 0490-5701 4.14 117.5 µM 8.95E-6 9 1.75E-5
9 SGTC_920 mycophenolic acid 4.07 780.4 nM 1.21E-5 25 2.33E-5
10 SGTC_1629 st005085 3.97 44.9 µM 1.94E-5 RPP1 & pyrimidine depletion 35 3.61E-5
11 SGTC_2464 5717415 3.72 7.7 µM 5.66E-5 23 9.81E-5
12 SGTC_918 glabranin 3.70 770.7 nM 6.28E-5 41 1.08E-4
13 SGTC_79 0052-0007 3.63 94.1 µM 8.43E-5 50 1.42E-4
14 SGTC_2628 lathosterol 3.36 100.0 µM 2.46E-4 50 3.87E-4
15 SGTC_2483 5729050 3.33 27.2 µM 2.76E-4 47 4.30E-4
16 SGTC_2949 9072542 3.29 2.2 µM 3.23E-4 TSC3-RPN4 69 4.99E-4
17 SGTC_1991 st073000 3.27 8.9 µM 3.54E-4 NEO1 116 5.43E-4
18 SGTC_2851 9015922 3.22 71.4 µM 4.25E-4 76 6.45E-4
19 SGTC_2603 homopterocarpin 3.22 100.0 µM 4.25E-4 88 6.45E-4
20 SGTC_3196 9111891 3.19 49.5 µM 4.74E-4 mitochondrial processes 40 7.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 5.79E-12 YDR055W PST1 hom Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
0.114 3.42E-11 YJR068W RFC2 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.110 1.97E-10 YDR089W YDR089W hom Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.107 4.35E-10 YOR098C NUP1 het FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
0.103 2.40E-9 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.094 4.96E-8 YPL114W_d YPL114W_d hom Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
0.086 5.89E-7 YPL216W_p YPL216W_p hom Putative protein of unknown function; YPL216W is not an essential gene
0.086 6.21E-7 YOL007C CSI2 hom Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
0.085 8.72E-7 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.084 1.05E-6 YLL028W TPO1 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
0.083 1.34E-6 YHR083W SAM35 het Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
0.083 1.66E-6 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.079 4.29E-6 YHR022C-A_p YHR022C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.079 5.23E-6 YER034W YER034W hom Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
0.078 6.07E-6 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm