HIPHOP chemogenomics database

YJR016C / ILV3

Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids

Zygosity: Heterozygous strain

fixedexpanded
Profile for YJR016C / ILV3

Click on Significant Values for Screen Details

Top fitness defect scores for YJR016C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_900	1112-0045	4.67	141.0 µM	1.91E-7	NEO1	4	1.52E-6	0.45
2	SGTC_884	0851-0618	4.40	334.0 µM	8.35E-7		4	5.32E-6	0.66
3	SGTC_23	0213-0011	4.03	40.0 µM	5.95E-6	copper-dependent oxidative stress	4	2.83E-5	0.30
4	SGTC_942	3253-1115	3.89	227.0 µM	1.15E-5		5	4.98E-5	0.03
5	SGTC_2794	7785382	3.63	27.3 µM	3.91E-5		17	1.41E-4	0.07
6	SGTC_119	0121-0043	3.49	24.8 µM	7.21E-5	mitochondrial stress	32	2.37E-4	0.02
7	SGTC_114	0364-0001	3.46	490.3 µM	8.23E-5		10	2.66E-4	0.03
8	SGTC_1176	0848-0131	3.24	83.3 µM	2.14E-4	Golgi	9	6.00E-4	0.07
9	SGTC_622	1348-1566	3.09	26.3 µM	3.92E-4		11	0.00101	0.00
10	SGTC_2335	9018011	3.08	154.2 µM	4.03E-4		20	0.00103	0.05
11	SGTC_1664	st014217	3.06	9.0 µM	4.43E-4	60S ribosome export	15	0.00112	0.07
12	SGTC_2505	galangin	3.05	7.9 µM	4.62E-4	RPP1 & pyrimidine depletion	12	0.00116	0.00
13	SGTC_1755	st041960	3.01	45.2 µM	5.23E-4		17	0.00129	0.11
14	SGTC_939	1165-0479	2.96	45.3 µM	6.48E-4		18	0.00155	0.03
15	SGTC_2491	paeonol	2.95	100.0 µM	6.65E-4		4	0.00158	0.08
16	SGTC_2115	tiliquinol	2.93	12.8 µM	7.18E-4		13	0.00169	0.01
17	SGTC_28	pentachloropyridine N-oxide	2.91	1.6 µM	7.84E-4	TSC3-RPN4	9	0.00182	0.03
18	SGTC_2891	9063876	2.90	19.5 µM	8.14E-4		6	0.00188	0.08
19	SGTC_439	amiodarone	2.85	34.4 µM	9.70E-4	NEO1-PIK1	20	0.00219	0.01
20	SGTC_649	1123-0311	2.85	367.0 µM	9.79E-4	endomembrane recycling	28	0.00221	0.04

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.163	2.69E-21	YER157W	COG3	het	Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.145	3.40E-17	YGL232W	TAN1	hom	Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.122	1.47E-12	YNL245C	CWC25	het	Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
0.117	1.14E-11	YBR043C	QDR3	hom	Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
0.115	2.33E-11	YFL021W	GAT1	hom	Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p
0.115	2.52E-11	YLR444C_d	YLR444C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.115	2.89E-11	YKL010C	UFD4	hom	Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
0.112	7.70E-11	YFR027W	ECO1	het	Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.111	1.27E-10	YMR074C	YMR074C	hom	Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
0.108	2.91E-10	YDL072C	YET3	hom	Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.107	4.31E-10	YOR245C	DGA1	hom	Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.105	1.16E-9	YPR132W	RPS23B	hom	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.103	2.16E-9	YGR209C	TRX2	hom	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.102	3.07E-9	YFL009W	CDC4	het	F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.102	3.20E-9	YDR120C	TRM1	hom	tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments