YJR031C / GEA1

Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR031C / GEA1

Click on Significant Values for Screen Details ID:SGTC_128|Compound:0906-2981|FD-Score:3.53|P-value:1.03E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:5.32|P-value:1.14E-8 ID:SGTC_134|Compound:0269-0018|FD-Score:-4.08|P-value:1.03E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.46|P-value:1.39E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-6.03|P-value:1.50E-10 ID:SGTC_164|Compound:4100-3994|FD-Score:4.29|P-value:3.25E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.37|P-value:1.96E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:2.94|P-value:9.82E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.34|P-value:2.19E-4 ID:SGTC_499|Compound:genistein|FD-Score:2.95|P-value:9.46E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.74|P-value:4.80E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:3.92|P-value:1.88E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:3.12|P-value:5.20E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.64|P-value:6.37E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.20|P-value:4.30E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.40|P-value:1.99E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.08|P-value:5.80E-8 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.23|P-value:3.36E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:2.95|P-value:9.50E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.10|P-value:5.57E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.71|P-value:4.75E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.63|P-value:6.71E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.12|P-value:5.16E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.03|P-value:7.06E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.29|P-value:3.05E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:5.15|P-value:3.21E-8 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.19|P-value:3.98E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.09|P-value:4.47E-8 ID:SGTC_1518|Compound:st038567|FD-Score:-3.14|P-value:5.39E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-3.33|P-value:2.63E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:2.95|P-value:9.38E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.17|P-value:5.80E-6 ID:SGTC_1734|Compound:st037168|FD-Score:-4.90|P-value:1.55E-7 ID:SGTC_1743|Compound:st037606|FD-Score:-3.59|P-value:9.25E-5 ID:SGTC_1747|Compound:st037496|FD-Score:-5.40|P-value:8.50E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-3.32|P-value:2.72E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.55|P-value:1.08E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.32|P-value:2.76E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-3.02|P-value:8.41E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.12|P-value:5.24E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-4.60|P-value:8.00E-7 ID:SGTC_2299|Compound:7976368|FD-Score:4.51|P-value:1.07E-6 ID:SGTC_2364|Compound:9055074|FD-Score:-3.67|P-value:6.41E-5 ID:SGTC_2366|Compound:9070441|FD-Score:-3.12|P-value:5.70E-4 ID:SGTC_2367|Compound:9070478|FD-Score:-3.58|P-value:9.36E-5 ID:SGTC_2416|Compound:tofa|FD-Score:2.96|P-value:9.23E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.34|P-value:2.18E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.08|P-value:6.06E-4 ID:SGTC_2591|Compound:skatole|FD-Score:-4.65|P-value:6.19E-7 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.32|P-value:2.66E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:-3.23|P-value:3.76E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:-3.43|P-value:1.75E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.35|P-value:2.14E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.60|P-value:7.59E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-4.00|P-value:1.55E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-4.30|P-value:3.62E-6 ID:SGTC_128|Compound:0906-2981|FD-Score:3.53|P-value:1.03E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:5.32|P-value:1.14E-8 ID:SGTC_134|Compound:0269-0018|FD-Score:-4.08|P-value:1.03E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.46|P-value:1.39E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-6.03|P-value:1.50E-10 ID:SGTC_164|Compound:4100-3994|FD-Score:4.29|P-value:3.25E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.37|P-value:1.96E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:2.94|P-value:9.82E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.34|P-value:2.19E-4 ID:SGTC_499|Compound:genistein|FD-Score:2.95|P-value:9.46E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.74|P-value:4.80E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:3.92|P-value:1.88E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:3.12|P-value:5.20E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.64|P-value:6.37E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.20|P-value:4.30E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.40|P-value:1.99E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.08|P-value:5.80E-8 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.23|P-value:3.36E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:2.95|P-value:9.50E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.10|P-value:5.57E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.71|P-value:4.75E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.63|P-value:6.71E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.12|P-value:5.16E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.03|P-value:7.06E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.29|P-value:3.05E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:5.15|P-value:3.21E-8 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.19|P-value:3.98E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:5.09|P-value:4.47E-8 ID:SGTC_1518|Compound:st038567|FD-Score:-3.14|P-value:5.39E-4 ID:SGTC_1519|Compound:esterone|FD-Score:-3.33|P-value:2.63E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:2.95|P-value:9.38E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.17|P-value:5.80E-6 ID:SGTC_1734|Compound:st037168|FD-Score:-4.90|P-value:1.55E-7 ID:SGTC_1743|Compound:st037606|FD-Score:-3.59|P-value:9.25E-5 ID:SGTC_1747|Compound:st037496|FD-Score:-5.40|P-value:8.50E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-3.32|P-value:2.72E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.55|P-value:1.08E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-3.32|P-value:2.76E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-3.02|P-value:8.41E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.12|P-value:5.24E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-4.60|P-value:8.00E-7 ID:SGTC_2299|Compound:7976368|FD-Score:4.51|P-value:1.07E-6 ID:SGTC_2364|Compound:9055074|FD-Score:-3.67|P-value:6.41E-5 ID:SGTC_2366|Compound:9070441|FD-Score:-3.12|P-value:5.70E-4 ID:SGTC_2367|Compound:9070478|FD-Score:-3.58|P-value:9.36E-5 ID:SGTC_2416|Compound:tofa|FD-Score:2.96|P-value:9.23E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.34|P-value:2.18E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.08|P-value:6.06E-4 ID:SGTC_2591|Compound:skatole|FD-Score:-4.65|P-value:6.19E-7 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.32|P-value:2.66E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:-3.23|P-value:3.76E-4 ID:SGTC_2716|Compound:st080858|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:-3.43|P-value:1.75E-4 ID:SGTC_2863|Compound:9028737|FD-Score:3.35|P-value:2.14E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.60|P-value:7.59E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-4.00|P-value:1.55E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-4.30|P-value:3.62E-6

Top fitness defect scores for YJR031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_130 0136-0219 5.32 337.7 µM 1.14E-8 7 5.11E-8
2 SGTC_1315 1237-0019 5.15 3.7 µM 3.21E-8 4 1.31E-7
3 SGTC_1369 1866-0067 5.09 208.0 µM 4.47E-8 RPP1 & pyrimidine depletion 19 1.77E-7
4 SGTC_2299 7976368 4.51 114.9 µM 1.07E-6 18 3.22E-6
5 SGTC_164 4100-3994 4.29 89.6 µM 3.25E-6 21 8.87E-6
6 SGTC_1607 st000305 4.17 50.6 µM 5.80E-6 8 1.50E-5
7 SGTC_637 1181-0519 3.92 7.3 µM 1.88E-5 fatty acid desaturase (OLE1) 104 4.40E-5
8 SGTC_1231 0242-0792 3.71 125.0 µM 4.75E-5 24 1.03E-4
9 SGTC_1053 3366-3204 3.64 134.0 µM 6.37E-5 cell wall 46 1.34E-4
10 SGTC_1241 0364-0022 3.63 50.8 µM 6.71E-5 ERG2 41 1.41E-4
11 SGTC_2966 9084041 3.60 48.0 µM 7.59E-5 45 1.57E-4
12 SGTC_128 0906-2981 3.53 13.4 µM 1.03E-4 24 2.08E-4
13 SGTC_138 0850-0259 3.46 40.9 µM 1.39E-4 cell wall 228 2.73E-4
14 SGTC_331 k048-0133 3.44 24.5 µM 1.49E-4 RSC & ERG11 20 2.91E-4
15 SGTC_278 4092-0821 3.37 23.9 µM 1.96E-4 plasma membrane duress 65 3.75E-4
16 SGTC_2863 9028737 3.35 58.4 µM 2.14E-4 16 4.06E-4
17 SGTC_2474 5321570 3.34 43.3 µM 2.18E-4 31 4.14E-4
18 SGTC_395 anisomycin 3.34 6.2 µM 2.19E-4 19 4.15E-4
19 SGTC_1183 1254-0324 3.23 198.0 µM 3.36E-4 49 6.13E-4
20 SGTC_2716 st080858 3.21 38.6 µM 3.67E-4 60S ribosome export 40 6.65E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.47E-12 YJL130C URA2 hom Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
0.116 1.63E-11 YIR014W_p YIR014W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
0.110 2.02E-10 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.106 7.61E-10 YOR286W RDL2 hom Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
0.104 1.65E-9 YMR254C_d YMR254C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.100 5.71E-9 YPL130W SPO19 hom Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation
0.094 4.57E-8 YLR022C SDO1 het Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
0.091 1.19E-7 YIL138C TPM2 hom Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
0.090 1.80E-7 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.084 9.67E-7 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
0.082 2.05E-6 YNL044W YIP3 hom Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
0.078 5.56E-6 YDL070W BDF2 hom Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress
0.078 6.20E-6 YPL194W DDC1 hom DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
0.077 6.99E-6 YMR025W CSI1 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
0.077 7.35E-6 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate