HIPHOP chemogenomics database

YJR043C / POL32

Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p

Zygosity: Homozygous strain

fixedexpanded
Profile for YJR043C / POL32

Click on Significant Values for Screen Details

Top fitness defect scores for YJR043C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_86	0417-1630	9.50	471.9 µM	2.93E-18	ubiquinone biosynthesis & proteasome	45	1.02E-21
2	SGTC_177	1327-0068	9.42	8.4 µM	5.75E-18		8	2.31E-21
3	SGTC_2344	7990960	8.49	77.0 µM	6.09E-15		3	1.03E-17
4	SGTC_771	0250-0039	7.94	27.3 µM	2.72E-13		2	1.01E-15
5	SGTC_2233	sulbentine	7.55	40.4 µM	3.55E-12		15	2.24E-14
6	SGTC_2619	obliquin	6.86	87.9 µM	2.25E-10	cell wall	62	3.35E-12
7	SGTC_2408	sulbentine	6.64	18.4 µM	8.06E-10		16	1.56E-11
8	SGTC_428	3448-4153	6.40	16.5 µM	3.02E-9	TSC3-RPN4	7	7.65E-11
9	SGTC_142	0335-0849	6.01	62.7 µM	2.35E-8	ubiquinone biosynthesis & proteasome	63	9.06E-10
10	SGTC_3135	9127742	5.77	49.5 µM	7.82E-8		4	3.85E-9
11	SGTC_212	3807-4682	5.75	38.8 µM	8.63E-8	tubulin folding & SWR complex	30	4.34E-9
12	SGTC_247	mechlorethamine	5.62	29.7 µM	1.63E-7	DNA damage response	6	9.32E-9
13	SGTC_87	0100-0052	5.39	35.5 µM	4.82E-7		45	3.44E-8
14	SGTC_3299	9121982	5.36	43.1 µM	5.67E-7		9	4.18E-8
15	SGTC_2023	5144124	5.19	124.0 µM	1.22E-6		6	1.05E-7
16	SGTC_2323	6148992	5.19	148.3 µM	1.22E-6		11	1.06E-7
17	SGTC_2484	5809818	5.10	12.0 µM	1.78E-6		8	1.65E-7
18	SGTC_1056	3448-1962	4.94	115.0 µM	3.56E-6	amide catabolism	10	3.82E-7
19	SGTC_773	4358-1457	4.94	44.6 µM	3.67E-6		20	3.95E-7
20	SGTC_64	000l-0018	4.89	272.1 µM	4.43E-6	ubiquinone biosynthesis & proteasome	112	4.96E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.219	1.05E-37	YDR204W	COQ4	hom	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.214	4.06E-36	YLR202C_d	YLR202C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.209	2.50E-34	YPR146C_d	YPR146C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.208	3.12E-34	YIL090W	ICE2	hom	Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
0.208	3.86E-34	YBR003W	COQ1	hom	Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.208	3.90E-34	YOR254C	SEC63	het	Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.203	1.53E-32	YNR041C	COQ2	hom	Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.201	1.05E-31	YGL119W	COQ8	hom	Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.200	1.81E-31	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.196	1.67E-30	YJL168C	SET2	hom	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.194	6.46E-30	YOL096C	COQ3	hom	O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.193	1.49E-29	YOR125C	CAT5	hom	Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.188	6.75E-28	YLR201C	COQ9	hom	Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.187	9.71E-28	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.187	1.12E-27	YJL193W_p	YJL193W_p	hom	Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect