YJR060W / CBF1

Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR060W / CBF1

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-4.32|P-value:1.24E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.73|P-value:6.35E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:5.68|P-value:5.11E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:4.51|P-value:5.04E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.76|P-value:5.83E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:6.84|P-value:2.01E-9 ID:SGTC_172|Compound:1112-0046|FD-Score:7.20|P-value:2.97E-10 ID:SGTC_178|Compound:4092-0806|FD-Score:15.00|P-value:4.60E-38 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.77|P-value:6.99E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:8.16|P-value:1.21E-12 ID:SGTC_207|Compound:4185-0010|FD-Score:7.27|P-value:2.03E-10 ID:SGTC_218|Compound:alverine citrate|FD-Score:6.55|P-value:8.67E-9 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.91|P-value:3.71E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:4.04|P-value:2.42E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.28|P-value:1.09E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:6.17|P-value:5.60E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:6.53|P-value:9.86E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:5.53|P-value:9.55E-7 ID:SGTC_332|Compound:4112-0506|FD-Score:4.25|P-value:1.23E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.96|P-value:9.57E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:4.63|P-value:3.28E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:10.20|P-value:7.87E-19 ID:SGTC_384|Compound:k007-0144|FD-Score:5.86|P-value:2.22E-7 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.90|P-value:1.19E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:4.32|P-value:9.79E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:5.09|P-value:5.73E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:4.37|P-value:8.12E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.74|P-value:6.17E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:4.85|P-value:1.46E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:4.09|P-value:2.04E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.79|P-value:5.31E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.03|P-value:2.55E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:5.28|P-value:2.73E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:6.53|P-value:9.45E-9 ID:SGTC_794|Compound:k038-0031|FD-Score:4.08|P-value:2.13E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:-4.61|P-value:4.50E-5 ID:SGTC_846|Compound:0335-0850|FD-Score:5.42|P-value:1.50E-6 ID:SGTC_861|Compound:0443-0273|FD-Score:-4.04|P-value:3.11E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.59|P-value:9.50E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.60|P-value:9.40E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.87|P-value:5.19E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.68|P-value:7.32E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.24|P-value:1.63E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.61|P-value:9.06E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.29|P-value:1.08E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.77|P-value:5.71E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.64|P-value:3.10E-5 ID:SGTC_1383|Compound:3049-0032|FD-Score:-7.66|P-value:3.58E-11 ID:SGTC_1384|Compound:3089-0840|FD-Score:9.84|P-value:1.46E-17 ID:SGTC_1393|Compound:3470-5649|FD-Score:-14.30|P-value:2.58E-34 ID:SGTC_1394|Compound:3474-0001|FD-Score:7.43|P-value:8.45E-11 ID:SGTC_1399|Compound:3562-5772|FD-Score:-5.58|P-value:1.05E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.63|P-value:3.31E-5 ID:SGTC_1797|Compound:st048695|FD-Score:4.85|P-value:1.46E-5 ID:SGTC_1857|Compound:st056007|FD-Score:4.03|P-value:2.54E-4 ID:SGTC_1884|Compound:5633444|FD-Score:6.64|P-value:5.52E-9 ID:SGTC_1911|Compound:st060208|FD-Score:-5.75|P-value:5.21E-7 ID:SGTC_1916|Compound:st067606|FD-Score:7.03|P-value:7.32E-10 ID:SGTC_1968|Compound:st069590|FD-Score:-3.66|P-value:9.58E-4 ID:SGTC_2013|Compound:4024628|FD-Score:5.56|P-value:8.36E-7 ID:SGTC_2036|Compound:5160441|FD-Score:6.36|P-value:2.18E-8 ID:SGTC_2050|Compound:5263056|FD-Score:5.30|P-value:2.44E-6 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.61|P-value:3.54E-5 ID:SGTC_2240|Compound:6690706|FD-Score:5.98|P-value:1.31E-7 ID:SGTC_2241|Compound:6772625|FD-Score:4.05|P-value:2.38E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.38|P-value:7.89E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.96|P-value:9.51E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.88|P-value:2.10E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:4.78|P-value:1.90E-5 ID:SGTC_2440|Compound:5628896|FD-Score:4.45|P-value:6.25E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.42|P-value:1.55E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.85|P-value:4.45E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.61|P-value:8.95E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.79|P-value:5.29E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.17|P-value:4.29E-6 ID:SGTC_2887|Compound:9044784|FD-Score:5.42|P-value:1.52E-6 ID:SGTC_2993|Compound:9054257|FD-Score:8.93|P-value:8.48E-15 ID:SGTC_3081|Compound:9117243|FD-Score:9.81|P-value:1.84E-17 ID:SGTC_3084|Compound:9116877|FD-Score:-3.97|P-value:3.82E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.71|P-value:6.77E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:-4.32|P-value:1.24E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.73|P-value:6.35E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:5.68|P-value:5.11E-7 ID:SGTC_119|Compound:0121-0043|FD-Score:4.51|P-value:5.04E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.76|P-value:5.83E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:6.84|P-value:2.01E-9 ID:SGTC_172|Compound:1112-0046|FD-Score:7.20|P-value:2.97E-10 ID:SGTC_178|Compound:4092-0806|FD-Score:15.00|P-value:4.60E-38 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.77|P-value:6.99E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:8.16|P-value:1.21E-12 ID:SGTC_207|Compound:4185-0010|FD-Score:7.27|P-value:2.03E-10 ID:SGTC_218|Compound:alverine citrate|FD-Score:6.55|P-value:8.67E-9 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.91|P-value:3.71E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:4.04|P-value:2.42E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.28|P-value:1.09E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:6.17|P-value:5.60E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:6.53|P-value:9.86E-9 ID:SGTC_320|Compound:4092-0846|FD-Score:5.53|P-value:9.55E-7 ID:SGTC_332|Compound:4112-0506|FD-Score:4.25|P-value:1.23E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.96|P-value:9.57E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:4.63|P-value:3.28E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:10.20|P-value:7.87E-19 ID:SGTC_384|Compound:k007-0144|FD-Score:5.86|P-value:2.22E-7 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.90|P-value:1.19E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:4.32|P-value:9.79E-5 ID:SGTC_515|Compound:4092-1064|FD-Score:5.09|P-value:5.73E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:4.37|P-value:8.12E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.74|P-value:6.17E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:4.85|P-value:1.46E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:4.09|P-value:2.04E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.79|P-value:5.31E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.03|P-value:2.55E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:5.28|P-value:2.73E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:6.53|P-value:9.45E-9 ID:SGTC_794|Compound:k038-0031|FD-Score:4.08|P-value:2.13E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:-4.61|P-value:4.50E-5 ID:SGTC_846|Compound:0335-0850|FD-Score:5.42|P-value:1.50E-6 ID:SGTC_861|Compound:0443-0273|FD-Score:-4.04|P-value:3.11E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.59|P-value:9.50E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:3.60|P-value:9.40E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.87|P-value:5.19E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.68|P-value:7.32E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.24|P-value:1.63E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.61|P-value:9.06E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:4.29|P-value:1.08E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.77|P-value:5.71E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.64|P-value:3.10E-5 ID:SGTC_1383|Compound:3049-0032|FD-Score:-7.66|P-value:3.58E-11 ID:SGTC_1384|Compound:3089-0840|FD-Score:9.84|P-value:1.46E-17 ID:SGTC_1393|Compound:3470-5649|FD-Score:-14.30|P-value:2.58E-34 ID:SGTC_1394|Compound:3474-0001|FD-Score:7.43|P-value:8.45E-11 ID:SGTC_1399|Compound:3562-5772|FD-Score:-5.58|P-value:1.05E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.63|P-value:3.31E-5 ID:SGTC_1797|Compound:st048695|FD-Score:4.85|P-value:1.46E-5 ID:SGTC_1857|Compound:st056007|FD-Score:4.03|P-value:2.54E-4 ID:SGTC_1884|Compound:5633444|FD-Score:6.64|P-value:5.52E-9 ID:SGTC_1911|Compound:st060208|FD-Score:-5.75|P-value:5.21E-7 ID:SGTC_1916|Compound:st067606|FD-Score:7.03|P-value:7.32E-10 ID:SGTC_1968|Compound:st069590|FD-Score:-3.66|P-value:9.58E-4 ID:SGTC_2013|Compound:4024628|FD-Score:5.56|P-value:8.36E-7 ID:SGTC_2036|Compound:5160441|FD-Score:6.36|P-value:2.18E-8 ID:SGTC_2050|Compound:5263056|FD-Score:5.30|P-value:2.44E-6 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.61|P-value:3.54E-5 ID:SGTC_2240|Compound:6690706|FD-Score:5.98|P-value:1.31E-7 ID:SGTC_2241|Compound:6772625|FD-Score:4.05|P-value:2.38E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.38|P-value:7.89E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.96|P-value:9.51E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.88|P-value:2.10E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:4.78|P-value:1.90E-5 ID:SGTC_2440|Compound:5628896|FD-Score:4.45|P-value:6.25E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.42|P-value:1.55E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.85|P-value:4.45E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.61|P-value:8.95E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.79|P-value:5.29E-4 ID:SGTC_2851|Compound:9015922|FD-Score:5.17|P-value:4.29E-6 ID:SGTC_2887|Compound:9044784|FD-Score:5.42|P-value:1.52E-6 ID:SGTC_2993|Compound:9054257|FD-Score:8.93|P-value:8.48E-15 ID:SGTC_3081|Compound:9117243|FD-Score:9.81|P-value:1.84E-17 ID:SGTC_3084|Compound:9116877|FD-Score:-3.97|P-value:3.82E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.71|P-value:6.77E-4

Top fitness defect scores for YJR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_178 4092-0806 15.00 87.5 µM 4.60E-38 ergosterol depletion effects on membrane 4 5.14E-51
2 SGTC_373 0844-0104 10.20 25.9 µM 7.87E-19 ERAD & cell cycle 8 7.19E-25
3 SGTC_1384 3089-0840 9.84 101.0 µM 1.46E-17 1 3.77E-23
4 SGTC_3081 9117243 9.81 49.5 µM 1.84E-17 fatty acid desaturase (OLE1) 3 5.17E-23
5 SGTC_2993 9054257 8.93 71.4 µM 8.48E-15 fatty acid desaturase (OLE1) 8 2.12E-19
6 SGTC_201 4262-0159 8.16 12.8 µM 1.21E-12 ergosterol depletion effects on membrane 9 1.74E-16
7 SGTC_1394 3474-0001 7.43 65.7 µM 8.45E-11 endomembrane recycling 7 5.46E-14
8 SGTC_207 4185-0010 7.27 4.5 µM 2.03E-10 endomembrane recycling 34 1.79E-13
9 SGTC_172 1112-0046 7.20 2.6 µM 2.97E-10 NEO1 6 2.99E-13
10 SGTC_1916 st067606 7.03 49.2 µM 7.32E-10 3 1.01E-12
11 SGTC_137 0438-0332 6.84 85.4 µM 2.01E-9 ubiquinone biosynthesis & proteasome 53 3.97E-12
12 SGTC_1884 5633444 6.64 25.0 µM 5.52E-9 redox potentiating 24 1.55E-11
13 SGTC_218 alverine citrate 6.55 93.8 µM 8.67E-9 fatty acid desaturase (OLE1) 10 2.84E-11
14 SGTC_768 0384-0033 6.53 77.3 µM 9.45E-9 fatty acid desaturase (OLE1) 32 3.19E-11
15 SGTC_312 4092-0653 6.53 6.8 µM 9.86E-9 ergosterol depletion effects on membrane 30 3.38E-11
16 SGTC_2036 5160441 6.36 105.0 µM 2.18E-8 NEO1 40 9.84E-11
17 SGTC_278 4092-0821 6.17 23.9 µM 5.60E-8 plasma membrane duress 15 3.52E-10
18 SGTC_2240 6690706 5.98 185.9 µM 1.31E-7 2 1.10E-9
19 SGTC_2250 alverine citrate 5.88 130.0 µM 2.10E-7 55 2.08E-9
20 SGTC_384 k007-0144 5.86 5.2 µM 2.22E-7 ERAD & cell cycle 26 2.25E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.422 2.62E-145 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.411 3.33E-137 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.371 6.40E-110 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.328 4.64E-85 YER031C YPT31 hom Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.303 2.60E-72 YOR070C GYP1 hom Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.290 4.39E-66 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.264 1.09E-54 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.264 1.29E-54 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.242 8.26E-46 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.230 1.85E-41 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.228 1.24E-40 YJL207C LAA1 hom AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.223 4.95E-39 YDR213W UPC2 hom Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
0.215 1.49E-36 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.211 4.04E-35 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.203 1.80E-32 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria