YJR099W / YUH1

Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR099W / YUH1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:5.79|P-value:1.02E-10 ID:SGTC_77|Compound:0828-0286|FD-Score:-4.54|P-value:2.85E-7 ID:SGTC_213|Compound:1246-0773|FD-Score:2.86|P-value:8.51E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.59|P-value:4.18E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:2.85|P-value:9.15E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-2.90|P-value:6.87E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.62|P-value:3.58E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:2.83|P-value:9.52E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:2.87|P-value:8.27E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.97|P-value:5.72E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.87|P-value:8.36E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.59|P-value:4.10E-5 ID:SGTC_2122|Compound:5258151|FD-Score:-3.47|P-value:6.53E-5 ID:SGTC_2127|Compound:5253358|FD-Score:-3.49|P-value:5.90E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.78|P-value:1.57E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-2.97|P-value:5.18E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.78|P-value:1.56E-5 ID:SGTC_2371|Compound:9071574|FD-Score:3.51|P-value:5.91E-5 ID:SGTC_2372|Compound:9071633|FD-Score:2.90|P-value:7.51E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.24|P-value:1.70E-6 ID:SGTC_2374|Compound:9071832|FD-Score:3.32|P-value:1.35E-4 ID:SGTC_2379|Compound:9035213|FD-Score:4.50|P-value:3.89E-7 ID:SGTC_2423|Compound:st077119|FD-Score:-5.32|P-value:2.36E-9 ID:SGTC_2424|Compound:5537928|FD-Score:-2.84|P-value:8.77E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.53|P-value:3.43E-7 ID:SGTC_2508|Compound:celastrol|FD-Score:-2.88|P-value:7.40E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.01|P-value:4.45E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.02|P-value:4.25E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.07|P-value:3.97E-6 ID:SGTC_2956|Compound:9076196|FD-Score:3.50|P-value:6.25E-5 ID:SGTC_3093|Compound:9117344|FD-Score:-3.34|P-value:1.17E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.42|P-value:5.69E-7 ID:SGTC_3181|Compound:9108277|FD-Score:-3.08|P-value:3.37E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:5.79|P-value:1.02E-10 ID:SGTC_77|Compound:0828-0286|FD-Score:-4.54|P-value:2.85E-7 ID:SGTC_213|Compound:1246-0773|FD-Score:2.86|P-value:8.51E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.59|P-value:4.18E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:2.85|P-value:9.15E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-2.90|P-value:6.87E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.62|P-value:3.58E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:2.83|P-value:9.52E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:2.87|P-value:8.27E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:2.97|P-value:5.72E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.87|P-value:8.36E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.59|P-value:4.10E-5 ID:SGTC_2122|Compound:5258151|FD-Score:-3.47|P-value:6.53E-5 ID:SGTC_2127|Compound:5253358|FD-Score:-3.49|P-value:5.90E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.78|P-value:1.57E-5 ID:SGTC_2329|Compound:6138490|FD-Score:-2.97|P-value:5.18E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.78|P-value:1.56E-5 ID:SGTC_2371|Compound:9071574|FD-Score:3.51|P-value:5.91E-5 ID:SGTC_2372|Compound:9071633|FD-Score:2.90|P-value:7.51E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.24|P-value:1.70E-6 ID:SGTC_2374|Compound:9071832|FD-Score:3.32|P-value:1.35E-4 ID:SGTC_2379|Compound:9035213|FD-Score:4.50|P-value:3.89E-7 ID:SGTC_2423|Compound:st077119|FD-Score:-5.32|P-value:2.36E-9 ID:SGTC_2424|Compound:5537928|FD-Score:-2.84|P-value:8.77E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.53|P-value:3.43E-7 ID:SGTC_2508|Compound:celastrol|FD-Score:-2.88|P-value:7.40E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.01|P-value:4.45E-4 ID:SGTC_2512|Compound:diallyl trisulfide|FD-Score:-3.02|P-value:4.25E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.07|P-value:3.97E-6 ID:SGTC_2956|Compound:9076196|FD-Score:3.50|P-value:6.25E-5 ID:SGTC_3093|Compound:9117344|FD-Score:-3.34|P-value:1.17E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.42|P-value:5.69E-7 ID:SGTC_3181|Compound:9108277|FD-Score:-3.08|P-value:3.37E-4

Top fitness defect scores for YJR099W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_65 0798-0905 5.79 35.0 µM 1.02E-10 calcium & mitochondrial duress 37 3.43E-9
2 SGTC_2425 5628681 4.53 7.8 µM 3.43E-7 10 3.00E-6
3 SGTC_2379 9035213 4.50 200.0 µM 3.89E-7 7 3.33E-6
4 SGTC_2373 9071805 4.24 200.0 µM 1.70E-6 8 1.14E-5
5 SGTC_2568 diosmetin 4.07 100.0 µM 3.97E-6 21 2.32E-5
6 SGTC_753 1322-0200 3.62 289.0 µM 3.58E-5 30 1.46E-4
7 SGTC_1369 1866-0067 3.59 208.0 µM 4.10E-5 RPP1 & pyrimidine depletion 51 1.63E-4
8 SGTC_268 camptothecin 3.59 39.6 µM 4.18E-5 DNA damage response 88 1.66E-4
9 SGTC_2371 9071574 3.51 200.0 µM 5.91E-5 23 2.22E-4
10 SGTC_2956 9076196 3.50 2.5 µM 6.25E-5 33 2.33E-4
11 SGTC_2374 9071832 3.32 200.0 µM 1.35E-4 endomembrane recycling 20 4.45E-4
12 SGTC_1131 3454-3128 2.97 32.3 µM 5.72E-4 94 0.00150
13 SGTC_2372 9071633 2.90 200.0 µM 7.51E-4 53 0.00188
14 SGTC_929 2805-0021 2.87 398.0 µM 8.27E-4 69 0.00204
15 SGTC_1347 1493-0251 2.87 600.0 nM 8.36E-4 mitochondrial response to ROS 66 0.00206
16 SGTC_213 1246-0773 2.86 967.0 nM 8.51E-4 77 0.00209
17 SGTC_377 3970-0964 2.85 33.1 µM 9.15E-4 125 0.00222
18 SGTC_775 4577-0580 2.83 57.3 µM 9.52E-4 44 0.00229
19 SGTC_1348 1493-0373 2.80 93.8 µM 0.00109 72 0.00258
20 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 2.74 100.0 µM 0.00136 104 0.00310

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 9.07E-28 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.170 4.85E-23 YFL027C GYP8 hom GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
0.169 7.59E-23 YPL027W SMA1 hom Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p
0.166 2.88E-22 YBR139W YBR139W hom Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
0.160 9.11E-21 YBR108W AIM3 hom Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.158 3.06E-20 YKL187C FAT3 hom Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
0.155 1.30E-19 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.143 9.90E-17 YMR118C_p SHH3_p hom Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase
0.142 1.29E-16 YNL086W_p SNN1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes
0.127 1.32E-13 YBR239C_p ERT1_p hom Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition
0.126 2.39E-13 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.124 6.75E-13 YJL136W-A_p YJL136W-A_p hom Putative protein of unknown function; identified by SAGE
0.119 4.34E-12 YGL262W_p YGL262W_p hom Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
0.114 3.08E-11 YLR251W SYM1 hom Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
0.112 6.84E-11 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene