YJR116W_p / TDA4

Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR116W / TDA4

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-2.62|P-value:7.58E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.67|P-value:6.09E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.33|P-value:5.10E-8 ID:SGTC_428|Compound:3448-4153|FD-Score:3.08|P-value:7.29E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-3.05|P-value:1.08E-4 ID:SGTC_512|Compound:prima-1|FD-Score:2.82|P-value:2.48E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.06|P-value:4.09E-7 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.54|P-value:8.78E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-2.67|P-value:6.07E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-2.59|P-value:8.69E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:2.82|P-value:2.51E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.98|P-value:4.59E-10 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:2.68|P-value:4.77E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.51|P-value:7.78E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:-4.25|P-value:1.17E-7 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.93|P-value:6.50E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.33|P-value:2.07E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:-3.14|P-value:6.87E-5 ID:SGTC_1440|Compound:4100-1550|FD-Score:-2.56|P-value:9.60E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.08|P-value:7.23E-5 ID:SGTC_1563|Compound:conessine|FD-Score:2.84|P-value:2.33E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:2.50|P-value:9.98E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-2.55|P-value:9.92E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.98|P-value:4.88E-7 ID:SGTC_1854|Compound:st056233|FD-Score:2.51|P-value:9.55E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.55|P-value:8.20E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-2.57|P-value:9.21E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.94|P-value:1.44E-4 ID:SGTC_2372|Compound:9071633|FD-Score:2.65|P-value:5.42E-4 ID:SGTC_2386|Compound:9075165|FD-Score:2.51|P-value:9.84E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:2.68|P-value:4.67E-4 ID:SGTC_2414|Compound:st077232|FD-Score:2.92|P-value:1.57E-4 ID:SGTC_2415|Compound:st077333|FD-Score:6.42|P-value:1.65E-15 ID:SGTC_2416|Compound:tofa|FD-Score:-2.90|P-value:2.23E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.04|P-value:3.36E-7 ID:SGTC_2444|Compound:5485484|FD-Score:3.87|P-value:9.38E-7 ID:SGTC_2537|Compound:larixol|FD-Score:3.04|P-value:9.01E-5 ID:SGTC_2724|Compound:maprotiline|FD-Score:4.05|P-value:3.21E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:2.53|P-value:8.93E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.58|P-value:8.90E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-3.63|P-value:5.26E-6 ID:SGTC_2999|Compound:9069765|FD-Score:-2.68|P-value:5.85E-4 ID:SGTC_3084|Compound:9116877|FD-Score:2.76|P-value:3.25E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-2.92|P-value:1.97E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-2.87|P-value:2.55E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-2.86|P-value:2.66E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-2.67|P-value:6.14E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.08|P-value:7.29E-5 ID:SGTC_3309|Compound:9129340|FD-Score:2.51|P-value:9.76E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.38|P-value:1.61E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:-2.62|P-value:7.58E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.67|P-value:6.09E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.33|P-value:5.10E-8 ID:SGTC_428|Compound:3448-4153|FD-Score:3.08|P-value:7.29E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-3.05|P-value:1.08E-4 ID:SGTC_512|Compound:prima-1|FD-Score:2.82|P-value:2.48E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.06|P-value:4.09E-7 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.54|P-value:8.78E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-2.67|P-value:6.07E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-2.59|P-value:8.69E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:2.82|P-value:2.51E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.98|P-value:4.59E-10 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:2.68|P-value:4.77E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.51|P-value:7.78E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:-4.25|P-value:1.17E-7 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.93|P-value:6.50E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.33|P-value:2.07E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:-3.14|P-value:6.87E-5 ID:SGTC_1440|Compound:4100-1550|FD-Score:-2.56|P-value:9.60E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.08|P-value:7.23E-5 ID:SGTC_1563|Compound:conessine|FD-Score:2.84|P-value:2.33E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:2.50|P-value:9.98E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-2.55|P-value:9.92E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.98|P-value:4.88E-7 ID:SGTC_1854|Compound:st056233|FD-Score:2.51|P-value:9.55E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.55|P-value:8.20E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-2.57|P-value:9.21E-4 ID:SGTC_2305|Compound:7443522|FD-Score:2.94|P-value:1.44E-4 ID:SGTC_2372|Compound:9071633|FD-Score:2.65|P-value:5.42E-4 ID:SGTC_2386|Compound:9075165|FD-Score:2.51|P-value:9.84E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:2.68|P-value:4.67E-4 ID:SGTC_2414|Compound:st077232|FD-Score:2.92|P-value:1.57E-4 ID:SGTC_2415|Compound:st077333|FD-Score:6.42|P-value:1.65E-15 ID:SGTC_2416|Compound:tofa|FD-Score:-2.90|P-value:2.23E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.04|P-value:3.36E-7 ID:SGTC_2444|Compound:5485484|FD-Score:3.87|P-value:9.38E-7 ID:SGTC_2537|Compound:larixol|FD-Score:3.04|P-value:9.01E-5 ID:SGTC_2724|Compound:maprotiline|FD-Score:4.05|P-value:3.21E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:2.53|P-value:8.93E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.58|P-value:8.90E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-3.63|P-value:5.26E-6 ID:SGTC_2999|Compound:9069765|FD-Score:-2.68|P-value:5.85E-4 ID:SGTC_3084|Compound:9116877|FD-Score:2.76|P-value:3.25E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-2.92|P-value:1.97E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-2.87|P-value:2.55E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-2.86|P-value:2.66E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-2.67|P-value:6.14E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.08|P-value:7.29E-5 ID:SGTC_3309|Compound:9129340|FD-Score:2.51|P-value:9.76E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.38|P-value:1.61E-5

Top fitness defect scores for YJR116W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2415 st077333 6.42 57.7 µM 1.65E-15 9 6.81E-11
2 SGTC_996 3970-0799 4.98 1.8 µM 4.59E-10 55 3.12E-7
3 SGTC_219 mitoxanthrone 4.33 164.4 µM 5.10E-8 DNA intercalators 44 7.48E-6
4 SGTC_2724 maprotiline 4.05 69.6 µM 3.21E-7 ERG2 14 2.59E-5
5 SGTC_2419 paclitaxel 4.04 166.5 µM 3.36E-7 35 2.67E-5
6 SGTC_1851 st056236 3.98 18.9 µM 4.88E-7 14 3.44E-5
7 SGTC_1326 1431-2094 3.93 10.8 µM 6.50E-7 tubulin folding & SWR complex 37 4.18E-5
8 SGTC_2444 5485484 3.87 36.4 µM 9.38E-7 39 5.36E-5
9 SGTC_1125 tnp00248 3.51 1.3 µM 7.78E-6 50 2.25E-4
10 SGTC_3313 9133968 3.38 57.0 µM 1.61E-5 14 3.68E-4
11 SGTC_1333 1482-0047 3.33 286.0 µM 2.07E-5 34 4.37E-4
12 SGTC_1520 flindersine 3.08 88.0 µM 7.23E-5 68 0.00102
13 SGTC_428 3448-4153 3.08 16.5 µM 7.29E-5 TSC3-RPN4 76 0.00103
14 SGTC_3266 9136860 3.08 49.5 µM 7.29E-5 cell wall 81 0.00103
15 SGTC_2537 larixol 3.04 68.9 µM 9.01E-5 70 0.00119
16 SGTC_2305 7443522 2.94 69.2 µM 1.44E-4 52 0.00164
17 SGTC_2414 st077232 2.92 92.9 µM 1.57E-4 75 0.00174
18 SGTC_1563 conessine 2.84 56.1 µM 2.33E-4 71 0.00227
19 SGTC_512 prima-1 2.82 270.0 µM 2.48E-4 DNA intercalators 67 0.00237
20 SGTC_953 1188-0045 2.82 9.7 µM 2.51E-4 60S ribosome export 88 0.00239

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.303 4.35E-72 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.235 2.86E-43 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.216 1.21E-36 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.214 4.30E-36 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.204 6.18E-33 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.193 2.31E-29 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.188 6.24E-28 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.186 1.51E-27 YGR189C CRH1 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
0.184 6.03E-27 YPL262W FUM1 hom Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
0.181 3.43E-26 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.175 1.39E-24 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.174 2.49E-24 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.173 7.52E-24 YMR112C MED11 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
0.169 7.13E-23 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
0.156 1.25E-19 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane