YJR135C / MCM22

Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR135C / MCM22

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.00|P-value:4.60E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:2.87|P-value:8.77E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.05|P-value:4.65E-6 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.46|P-value:5.30E-7 ID:SGTC_229|Compound:benomyl|FD-Score:3.19|P-value:2.49E-4 ID:SGTC_230|Compound:MPP+|FD-Score:3.42|P-value:9.13E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.95|P-value:7.65E-6 ID:SGTC_264|Compound:promethazine|FD-Score:3.59|P-value:4.43E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:2.96|P-value:6.17E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.39|P-value:1.08E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.99|P-value:6.56E-6 ID:SGTC_825|Compound:1988-2224|FD-Score:3.72|P-value:2.33E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:3.32|P-value:1.46E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.09|P-value:3.66E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.45|P-value:8.23E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:4.72|P-value:1.22E-7 ID:SGTC_1445|Compound:4239-0156|FD-Score:-2.87|P-value:7.42E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.25|P-value:1.63E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.97|P-value:5.79E-6 ID:SGTC_1867|Compound:st057372|FD-Score:-3.14|P-value:2.63E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.38|P-value:9.28E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.49|P-value:4.37E-7 ID:SGTC_1881|Compound:5652484|FD-Score:3.27|P-value:1.79E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:2.92|P-value:7.11E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.24|P-value:1.97E-4 ID:SGTC_2098|Compound:5376439|FD-Score:3.43|P-value:9.02E-5 ID:SGTC_2100|Compound:5377183|FD-Score:3.03|P-value:4.63E-4 ID:SGTC_2102|Compound:5377816|FD-Score:2.83|P-value:9.88E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-2.80|P-value:9.87E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.39|P-value:8.98E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.48|P-value:6.18E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.61|P-value:3.97E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.82|P-value:1.48E-5 ID:SGTC_2819|Compound:7996655|FD-Score:-2.87|P-value:7.40E-4 ID:SGTC_2824|Compound:7998404|FD-Score:2.86|P-value:8.85E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.55|P-value:5.32E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.30|P-value:1.54E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.22|P-value:2.21E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.53|P-value:5.68E-5 ID:SGTC_3119|Compound:9125183|FD-Score:-3.52|P-value:5.08E-5 ID:SGTC_3130|Compound:9127414|FD-Score:3.44|P-value:8.42E-5 ID:SGTC_3216|Compound:9129716|FD-Score:-5.14|P-value:7.25E-9 ID:SGTC_3227|Compound:9130768|FD-Score:3.21|P-value:2.26E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-4.41|P-value:5.77E-7 ID:SGTC_3242|Compound:9133940|FD-Score:3.13|P-value:3.15E-4 ID:SGTC_3257|Compound:9139206|FD-Score:3.20|P-value:2.41E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.20|P-value:2.01E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.00|P-value:4.60E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:2.87|P-value:8.77E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.05|P-value:4.65E-6 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.46|P-value:5.30E-7 ID:SGTC_229|Compound:benomyl|FD-Score:3.19|P-value:2.49E-4 ID:SGTC_230|Compound:MPP+|FD-Score:3.42|P-value:9.13E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.95|P-value:7.65E-6 ID:SGTC_264|Compound:promethazine|FD-Score:3.59|P-value:4.43E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:2.96|P-value:6.17E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.39|P-value:1.08E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.99|P-value:6.56E-6 ID:SGTC_825|Compound:1988-2224|FD-Score:3.72|P-value:2.33E-5 ID:SGTC_827|Compound:3253-0474|FD-Score:3.32|P-value:1.46E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.09|P-value:3.66E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.45|P-value:8.23E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:4.72|P-value:1.22E-7 ID:SGTC_1445|Compound:4239-0156|FD-Score:-2.87|P-value:7.42E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.25|P-value:1.63E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.97|P-value:5.79E-6 ID:SGTC_1867|Compound:st057372|FD-Score:-3.14|P-value:2.63E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.38|P-value:9.28E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.49|P-value:4.37E-7 ID:SGTC_1881|Compound:5652484|FD-Score:3.27|P-value:1.79E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:2.92|P-value:7.11E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.24|P-value:1.97E-4 ID:SGTC_2098|Compound:5376439|FD-Score:3.43|P-value:9.02E-5 ID:SGTC_2100|Compound:5377183|FD-Score:3.03|P-value:4.63E-4 ID:SGTC_2102|Compound:5377816|FD-Score:2.83|P-value:9.88E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-2.80|P-value:9.87E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.39|P-value:8.98E-5 ID:SGTC_2694|Compound:st077685|FD-Score:-3.48|P-value:6.18E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.61|P-value:3.97E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.82|P-value:1.48E-5 ID:SGTC_2819|Compound:7996655|FD-Score:-2.87|P-value:7.40E-4 ID:SGTC_2824|Compound:7998404|FD-Score:2.86|P-value:8.85E-4 ID:SGTC_2854|Compound:9024559|FD-Score:3.55|P-value:5.32E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.30|P-value:1.54E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.22|P-value:2.21E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.53|P-value:5.68E-5 ID:SGTC_3119|Compound:9125183|FD-Score:-3.52|P-value:5.08E-5 ID:SGTC_3130|Compound:9127414|FD-Score:3.44|P-value:8.42E-5 ID:SGTC_3216|Compound:9129716|FD-Score:-5.14|P-value:7.25E-9 ID:SGTC_3227|Compound:9130768|FD-Score:3.21|P-value:2.26E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-4.41|P-value:5.77E-7 ID:SGTC_3242|Compound:9133940|FD-Score:3.13|P-value:3.15E-4 ID:SGTC_3257|Compound:9139206|FD-Score:3.20|P-value:2.41E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.20|P-value:2.01E-4

Top fitness defect scores for YJR135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1348 1493-0373 4.72 93.8 µM 1.22E-7 8 1.19E-6
2 SGTC_1875 nocodazole 4.49 6.0 µM 4.37E-7 tubulin folding & SWR complex 42 3.48E-6
3 SGTC_219 mitoxanthrone 4.46 164.4 µM 5.30E-7 DNA intercalators 41 4.09E-6
4 SGTC_145 3737-0113 4.05 38.4 µM 4.65E-6 DNA intercalators 70 2.52E-5
5 SGTC_781 4335-2806 3.99 94.4 µM 6.56E-6 20 3.37E-5
6 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.95 8.9 µM 7.65E-6 heat shock/prefoldin 42 3.84E-5
7 SGTC_2812 7970281 3.82 58.4 µM 1.48E-5 35 6.68E-5
8 SGTC_825 1988-2224 3.72 89.6 µM 2.33E-5 cell wall signaling 40 9.77E-5
9 SGTC_2810 7959871 3.61 45.5 µM 3.97E-5 copper-dependent oxidative stress 49 1.53E-4
10 SGTC_264 promethazine 3.59 12.5 mM 4.43E-5 DNA intercalators 66 1.68E-4
11 SGTC_2854 9024559 3.55 71.4 µM 5.32E-5 38 1.96E-4
12 SGTC_2880 9042983 3.53 58.4 µM 5.68E-5 fatty acid desaturase (OLE1) 41 2.07E-4
13 SGTC_1290 0986-0249 3.45 10.2 µM 8.23E-5 DNA intercalators 36 2.82E-4
14 SGTC_3130 9127414 3.44 49.5 µM 8.42E-5 27 2.88E-4
15 SGTC_2098 5376439 3.43 882.5 nM 9.02E-5 23 3.05E-4
16 SGTC_230 MPP+ 3.42 141.7 µM 9.13E-5 33 3.08E-4
17 SGTC_741 4582-0002 3.39 126.0 µM 1.08E-4 29 3.54E-4
18 SGTC_827 3253-0474 3.32 693.0 µM 1.46E-4 43 4.57E-4
19 SGTC_2856 9022925 3.30 19.5 µM 1.54E-4 47 4.78E-4
20 SGTC_1881 5652484 3.27 16.0 µM 1.79E-4 TRP & mitochondrial translation 56 5.43E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.247 5.36E-48 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.229 5.49E-41 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.197 9.28E-31 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.176 1.12E-24 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.173 7.37E-24 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.164 9.81E-22 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.163 2.48E-21 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.160 1.20E-20 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.140 3.95E-16 YGR069W_d YGR069W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.135 4.15E-15 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed
0.134 6.05E-15 YHL006C SHU1 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
0.133 9.37E-15 YJL008C CCT8 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.127 1.60E-13 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.127 1.84E-13 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.126 2.21E-13 YBR060C ORC2 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p