HIPHOP chemogenomics database

YJR135W-A / TIM8

Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome

Zygosity: Homozygous strain

fixedexpanded
Profile for YJR135W-A / TIM8

Click on Significant Values for Screen Details

Top fitness defect scores for YJR135W-A deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1635	st007035	6.57	42.4 µM	4.63E-12		125	2.56E-11
2	SGTC_361	000s-0510	6.19	1.4 µM	6.55E-11		4	3.03E-10
3	SGTC_778	3456-2434	5.69	196.0 µM	1.67E-9	mitochondrial response to ROS	27	6.22E-9
4	SGTC_1564	D-Arginine	5.32	115.0 µM	1.67E-8		12	5.33E-8
5	SGTC_236	1509-0011	5.30	5.3 µM	1.79E-8		309	5.69E-8
6	SGTC_1447	4239-0816	4.69	81.9 µM	5.39E-7		18	1.37E-6
7	SGTC_1456	k015-0012	4.67	13.0 µM	6.07E-7		17	1.53E-6
8	SGTC_2690	usnic acid	4.39	100.0 µM	2.44E-6		19	5.60E-6
9	SGTC_431	k089-0097	4.38	107.0 µM	2.54E-6	iron homeostasis	24	5.81E-6
10	SGTC_1344	1488-0097	4.33	29.1 µM	3.34E-6		11	7.50E-6
11	SGTC_426	0988-0037	4.32	14.6 µM	3.41E-6	ERAD & cell cycle	24	7.65E-6
12	SGTC_275	trichlorophene	4.20	5.7 µM	6.15E-6	mitochondrial stress	213	1.33E-5
13	SGTC_2443	5493264	3.96	126.7 µM	1.84E-5		21	3.68E-5
14	SGTC_3209	9113616	3.93	49.5 µM	2.18E-5		26	4.31E-5
15	SGTC_116	0155-0182	3.75	31.8 µM	4.63E-5	mitochondrial response to ROS	93	8.72E-5
16	SGTC_1138	3761-0006	3.75	1.0 µM	4.63E-5		6	8.72E-5
17	SGTC_141	0443-0274	3.68	32.3 µM	6.42E-5	mitochondrial response to ROS	54	1.18E-4
18	SGTC_922	1866-0035	3.63	480.0 µM	7.92E-5	excess fatty acid	75	1.44E-4
19	SGTC_1531	st059297	3.61	52.4 µM	8.54E-5		31	1.54E-4
20	SGTC_733	4069-0007	3.61	56.1 µM	8.60E-5	ERAD & cell cycle	96	1.55E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.286	1.92E-64	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.278	1.41E-60	YHR050W-A_p	YHR050W-A_p	hom	Protein of unknown function; identified by expression profiling and mass spectrometry
0.272	4.39E-58	YNL213C	RRG9	hom	Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.264	1.18E-54	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.261	2.59E-53	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.254	1.05E-50	YDR518W	EUG1	hom	Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.252	1.58E-49	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.249	1.74E-48	YGR183C	QCR9	hom	Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.248	2.00E-48	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.247	5.84E-48	YKL155C	RSM22	hom	Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.243	3.58E-46	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.237	3.88E-44	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.235	2.60E-43	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.231	5.34E-42	YGR112W	SHY1	hom	Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome
0.227	2.10E-40	YJL023C	PET130	hom	Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies