YJR135W-A / TIM8

Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR135W-A / TIM8

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.11|P-value:7.15E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.75|P-value:4.63E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.68|P-value:6.42E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:5.30|P-value:1.79E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.20|P-value:6.15E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.17|P-value:4.81E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:6.19|P-value:6.55E-11 ID:SGTC_384|Compound:k007-0144|FD-Score:3.59|P-value:9.27E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:2.97|P-value:9.59E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.32|P-value:3.41E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:4.38|P-value:2.54E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:3.32|P-value:2.65E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:3.21|P-value:4.04E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.61|P-value:8.60E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:3.23|P-value:3.85E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:5.69|P-value:1.67E-9 ID:SGTC_922|Compound:1866-0035|FD-Score:3.63|P-value:7.92E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.75|P-value:4.63E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:2.99|P-value:8.90E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:4.33|P-value:3.34E-6 ID:SGTC_1352|Compound:1496-0991|FD-Score:-3.24|P-value:4.55E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.02|P-value:8.24E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.69|P-value:5.39E-7 ID:SGTC_1453|Compound:4237-0541|FD-Score:2.98|P-value:9.46E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.67|P-value:6.07E-7 ID:SGTC_1531|Compound:st059297|FD-Score:3.61|P-value:8.54E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:5.32|P-value:1.67E-8 ID:SGTC_1635|Compound:st007035|FD-Score:6.57|P-value:4.63E-12 ID:SGTC_2072|Compound:5231554|FD-Score:-3.22|P-value:4.81E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.09|P-value:7.86E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.45|P-value:1.64E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-5.52|P-value:6.81E-9 ID:SGTC_2293|Compound:7624732|FD-Score:-4.18|P-value:9.10E-6 ID:SGTC_2387|Compound:9077560|FD-Score:-3.38|P-value:2.63E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.96|P-value:1.84E-5 ID:SGTC_2459|Compound:5811157|FD-Score:-3.31|P-value:3.52E-4 ID:SGTC_2472|Compound:5377652|FD-Score:-3.58|P-value:1.21E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-5.47|P-value:9.32E-9 ID:SGTC_2517|Compound:totarol|FD-Score:-3.83|P-value:4.28E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.39|P-value:2.44E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.69|P-value:7.81E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.10|P-value:6.08E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.93|P-value:2.18E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.98|P-value:2.25E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.11|P-value:7.15E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.75|P-value:4.63E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.68|P-value:6.42E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:5.30|P-value:1.79E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.20|P-value:6.15E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.17|P-value:4.81E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:6.19|P-value:6.55E-11 ID:SGTC_384|Compound:k007-0144|FD-Score:3.59|P-value:9.27E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:2.97|P-value:9.59E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.32|P-value:3.41E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:4.38|P-value:2.54E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:3.32|P-value:2.65E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:3.21|P-value:4.04E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.61|P-value:8.60E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:3.23|P-value:3.85E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:5.69|P-value:1.67E-9 ID:SGTC_922|Compound:1866-0035|FD-Score:3.63|P-value:7.92E-5 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.75|P-value:4.63E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:2.99|P-value:8.90E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:4.33|P-value:3.34E-6 ID:SGTC_1352|Compound:1496-0991|FD-Score:-3.24|P-value:4.55E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.02|P-value:8.24E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.69|P-value:5.39E-7 ID:SGTC_1453|Compound:4237-0541|FD-Score:2.98|P-value:9.46E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.67|P-value:6.07E-7 ID:SGTC_1531|Compound:st059297|FD-Score:3.61|P-value:8.54E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:5.32|P-value:1.67E-8 ID:SGTC_1635|Compound:st007035|FD-Score:6.57|P-value:4.63E-12 ID:SGTC_2072|Compound:5231554|FD-Score:-3.22|P-value:4.81E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.09|P-value:7.86E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.45|P-value:1.64E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-5.52|P-value:6.81E-9 ID:SGTC_2293|Compound:7624732|FD-Score:-4.18|P-value:9.10E-6 ID:SGTC_2387|Compound:9077560|FD-Score:-3.38|P-value:2.63E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.96|P-value:1.84E-5 ID:SGTC_2459|Compound:5811157|FD-Score:-3.31|P-value:3.52E-4 ID:SGTC_2472|Compound:5377652|FD-Score:-3.58|P-value:1.21E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-5.47|P-value:9.32E-9 ID:SGTC_2517|Compound:totarol|FD-Score:-3.83|P-value:4.28E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:4.39|P-value:2.44E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.69|P-value:7.81E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.10|P-value:6.08E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.93|P-value:2.18E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-3.98|P-value:2.25E-5

Top fitness defect scores for YJR135W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1635 st007035 6.57 42.4 µM 4.63E-12 125 2.56E-11
2 SGTC_361 000s-0510 6.19 1.4 µM 6.55E-11 4 3.03E-10
3 SGTC_778 3456-2434 5.69 196.0 µM 1.67E-9 mitochondrial response to ROS 27 6.22E-9
4 SGTC_1564 D-Arginine 5.32 115.0 µM 1.67E-8 12 5.33E-8
5 SGTC_236 1509-0011 5.30 5.3 µM 1.79E-8 309 5.69E-8
6 SGTC_1447 4239-0816 4.69 81.9 µM 5.39E-7 18 1.37E-6
7 SGTC_1456 k015-0012 4.67 13.0 µM 6.07E-7 17 1.53E-6
8 SGTC_2690 usnic acid 4.39 100.0 µM 2.44E-6 19 5.60E-6
9 SGTC_431 k089-0097 4.38 107.0 µM 2.54E-6 iron homeostasis 24 5.81E-6
10 SGTC_1344 1488-0097 4.33 29.1 µM 3.34E-6 11 7.50E-6
11 SGTC_426 0988-0037 4.32 14.6 µM 3.41E-6 ERAD & cell cycle 24 7.65E-6
12 SGTC_275 trichlorophene 4.20 5.7 µM 6.15E-6 mitochondrial stress 213 1.33E-5
13 SGTC_2443 5493264 3.96 126.7 µM 1.84E-5 21 3.68E-5
14 SGTC_3209 9113616 3.93 49.5 µM 2.18E-5 26 4.31E-5
15 SGTC_116 0155-0182 3.75 31.8 µM 4.63E-5 mitochondrial response to ROS 93 8.72E-5
16 SGTC_1138 3761-0006 3.75 1.0 µM 4.63E-5 6 8.72E-5
17 SGTC_141 0443-0274 3.68 32.3 µM 6.42E-5 mitochondrial response to ROS 54 1.18E-4
18 SGTC_922 1866-0035 3.63 480.0 µM 7.92E-5 excess fatty acid 75 1.44E-4
19 SGTC_1531 st059297 3.61 52.4 µM 8.54E-5 31 1.54E-4
20 SGTC_733 4069-0007 3.61 56.1 µM 8.60E-5 ERAD & cell cycle 96 1.55E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.286 1.92E-64 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.278 1.41E-60 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.272 4.39E-58 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.264 1.18E-54 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.261 2.59E-53 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.254 1.05E-50 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.252 1.58E-49 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.249 1.74E-48 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.248 2.00E-48 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.247 5.84E-48 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.243 3.58E-46 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.237 3.88E-44 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.235 2.60E-43 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.231 5.34E-42 YGR112W SHY1 hom Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome
0.227 2.10E-40 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies