YJR153W / PGU1

Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR153W / PGU1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.68|P-value:5.55E-7 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.93|P-value:3.32E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.52|P-value:2.09E-6 ID:SGTC_264|Compound:promethazine|FD-Score:-3.21|P-value:5.72E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.69|P-value:9.14E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:4.52|P-value:1.27E-6 ID:SGTC_644|Compound:1498-1197|FD-Score:-4.87|P-value:3.57E-7 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.61|P-value:1.26E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:5.21|P-value:2.91E-8 ID:SGTC_739|Compound:1486-1183|FD-Score:3.95|P-value:1.87E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:3.17|P-value:4.56E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.40|P-value:2.84E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.90|P-value:3.69E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.37|P-value:3.24E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.31|P-value:3.57E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.43|P-value:1.53E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.46|P-value:1.26E-8 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.24|P-value:3.43E-4 ID:SGTC_2010|Compound:4012422|FD-Score:4.12|P-value:8.73E-6 ID:SGTC_2267|Compound:7931562|FD-Score:-3.72|P-value:7.94E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.18|P-value:4.31E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.00|P-value:8.26E-4 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:-3.19|P-value:6.21E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.08|P-value:9.31E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.96|P-value:9.60E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.68|P-value:5.55E-7 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.93|P-value:3.32E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.52|P-value:2.09E-6 ID:SGTC_264|Compound:promethazine|FD-Score:-3.21|P-value:5.72E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.69|P-value:9.14E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:4.52|P-value:1.27E-6 ID:SGTC_644|Compound:1498-1197|FD-Score:-4.87|P-value:3.57E-7 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.61|P-value:1.26E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:5.21|P-value:2.91E-8 ID:SGTC_739|Compound:1486-1183|FD-Score:3.95|P-value:1.87E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:3.17|P-value:4.56E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.40|P-value:2.84E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.90|P-value:3.69E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.37|P-value:3.24E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.31|P-value:3.57E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.43|P-value:1.53E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.46|P-value:1.26E-8 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.24|P-value:3.43E-4 ID:SGTC_2010|Compound:4012422|FD-Score:4.12|P-value:8.73E-6 ID:SGTC_2267|Compound:7931562|FD-Score:-3.72|P-value:7.94E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.18|P-value:4.31E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.00|P-value:8.26E-4 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:-3.19|P-value:6.21E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.08|P-value:9.31E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.96|P-value:9.60E-4

Top fitness defect scores for YJR153W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_648 0375-0473 5.21 33.3 µM 2.91E-8 6 9.21E-8
2 SGTC_64 000l-0018 4.68 272.1 µM 5.55E-7 ubiquinone biosynthesis & proteasome 119 1.45E-6
3 SGTC_611 3453-1652 4.52 23.7 µM 1.27E-6 11 3.15E-6
4 SGTC_1420 4'-methoxyflavone 4.31 42.0 µM 3.57E-6 10 8.29E-6
5 SGTC_2010 4012422 4.12 159.0 µM 8.73E-6 20 1.91E-5
6 SGTC_739 1486-1183 3.95 203.0 µM 1.87E-5 17 3.90E-5
7 SGTC_1544 7-methoxy-4-methylcoumarin 3.24 105.0 µM 3.43E-4 40 5.89E-4
8 SGTC_2307 7538926 3.18 6.3 µM 4.31E-4 75 7.30E-4
9 SGTC_780 0109-0199 3.17 192.0 µM 4.56E-4 51 7.69E-4
10 SGTC_2379 9035213 3.00 200.0 µM 8.26E-4 58 0.00134
11 SGTC_2954 9074818 2.96 52.7 µM 9.60E-4 76 0.00154
12 SGTC_779 1360-0071 2.93 233.0 µM 0.00105 fatty acid desaturase (OLE1) 84 0.00168
13 SGTC_1390 3413-2614 2.89 145.0 µM 0.00121 fatty acid desaturase (OLE1) 75 0.00191
14 SGTC_558 k064-0027 2.89 21.3 µM 0.00121 DNA damage response 83 0.00191
15 SGTC_2375 9072683 2.84 200.0 µM 0.00146 mitochondrial processes 79 0.00228
16 SGTC_3327 9140648 2.81 26.9 µM 0.00160 53 0.00248
17 SGTC_2968 9090663 2.80 33.4 µM 0.00166 96 0.00257
18 SGTC_594 1487-1584 2.72 10.8 µM 0.00217 78 0.00330
19 SGTC_285 1486-1325 2.69 46.0 µM 0.00239 76 0.00361
20 SGTC_3213 9129884 2.68 49.5 µM 0.00246 76 0.00370

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.278 1.95E-60 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.275 4.23E-59 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.270 4.46E-57 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.260 7.03E-53 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.254 1.25E-50 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.253 6.41E-50 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.253 3.68E-50 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.251 2.50E-49 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.249 1.36E-48 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.248 2.57E-48 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.245 4.84E-47 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.239 1.28E-44 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.236 1.03E-43 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.234 8.39E-43 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.230 1.54E-41 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies