HIPHOP chemogenomics database

YJR153W / PGU1

Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins

Zygosity: Homozygous strain

fixedexpanded
Profile for YJR153W / PGU1

Click on Significant Values for Screen Details

Top fitness defect scores for YJR153W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_648	0375-0473	5.21	33.3 µM	2.91E-8		6	9.21E-8
2	SGTC_64	000l-0018	4.68	272.1 µM	5.55E-7	ubiquinone biosynthesis & proteasome	119	1.45E-6
3	SGTC_611	3453-1652	4.52	23.7 µM	1.27E-6		11	3.15E-6
4	SGTC_1420	4'-methoxyflavone	4.31	42.0 µM	3.57E-6		10	8.29E-6
5	SGTC_2010	4012422	4.12	159.0 µM	8.73E-6		20	1.91E-5
6	SGTC_739	1486-1183	3.95	203.0 µM	1.87E-5		17	3.90E-5
7	SGTC_1544	7-methoxy-4-methylcoumarin	3.24	105.0 µM	3.43E-4		40	5.89E-4
8	SGTC_2307	7538926	3.18	6.3 µM	4.31E-4		75	7.30E-4
9	SGTC_780	0109-0199	3.17	192.0 µM	4.56E-4		51	7.69E-4
10	SGTC_2379	9035213	3.00	200.0 µM	8.26E-4		58	0.00134
11	SGTC_2954	9074818	2.96	52.7 µM	9.60E-4		76	0.00154
12	SGTC_779	1360-0071	2.93	233.0 µM	0.00105	fatty acid desaturase (OLE1)	84	0.00168
13	SGTC_1390	3413-2614	2.89	145.0 µM	0.00121	fatty acid desaturase (OLE1)	75	0.00191
14	SGTC_558	k064-0027	2.89	21.3 µM	0.00121	DNA damage response	83	0.00191
15	SGTC_2375	9072683	2.84	200.0 µM	0.00146	mitochondrial processes	79	0.00228
16	SGTC_3327	9140648	2.81	26.9 µM	0.00160		53	0.00248
17	SGTC_2968	9090663	2.80	33.4 µM	0.00166		96	0.00257
18	SGTC_594	1487-1584	2.72	10.8 µM	0.00217		78	0.00330
19	SGTC_285	1486-1325	2.69	46.0 µM	0.00239		76	0.00361
20	SGTC_3213	9129884	2.68	49.5 µM	0.00246		76	0.00370

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.278	1.95E-60	YOR152C_p	YOR152C_p	hom	Putative protein of unknown function; YOR152C is not an essential gene
0.275	4.23E-59	YJL171C	YJL171C	hom	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.270	4.46E-57	YGR022C_d	YGR022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.260	7.03E-53	YNL166C	BNI5	hom	Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.254	1.25E-50	YFL014W	HSP12	hom	Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.253	6.41E-50	YPR180W	AOS1	het	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.253	3.68E-50	YJR058C	APS2	hom	Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.251	2.50E-49	YNL218W	MGS1	hom	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.249	1.36E-48	YJR137C	MET5	hom	Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.248	2.57E-48	YDR521W_d	YDR521W_d	hom	Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.245	4.84E-47	YGR136W	LSB1	hom	Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.239	1.28E-44	YDL176W	YDL176W	hom	Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.236	1.03E-43	YDR530C	APA2	hom	Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.234	8.39E-43	YGR077C	PEX8	hom	Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.230	1.54E-41	YDL157C_p	YDL157C_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies