HIPHOP chemogenomics database

YKL002W / DID4

Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL002W / DID4

Click on Significant Values for Screen Details

Top fitness defect scores for YKL002W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	8.60	54.9 µM	7.52E-16	redox potentiating	17	4.12E-18
2	SGTC_1658	st012944	7.51	20.0 µM	1.61E-12	iron homeostasis	12	3.05E-14
3	SGTC_1750	st081859	7.16	2.4 µM	1.45E-11	endomembrane recycling	85	3.92E-13
4	SGTC_2024	5144227	6.35	134.0 µM	1.81E-9		9	1.06E-10
5	SGTC_748	0136-0228	5.93	77.9 µM	1.79E-8	redox potentiating	38	1.51E-9
6	SGTC_37	0566-0070	5.89	1.6 µM	2.18E-8		6	1.90E-9
7	SGTC_1333	1482-0047	5.39	286.0 µM	2.73E-7		6	3.55E-8
8	SGTC_2296	7971656	5.25	126.5 µM	5.25E-7		20	7.55E-8
9	SGTC_2666	tannic acid	5.20	100.0 µM	6.76E-7	iron homeostasis	17	1.01E-7
10	SGTC_2679	rapamycin	5.05	1.0 nM	1.35E-6		95	2.26E-7
11	SGTC_214	4373-3496	5.04	70.0 µM	1.42E-6		28	2.39E-7
12	SGTC_2737	bleomycin	4.99	30.5 nM	1.72E-6	Golgi	54	2.97E-7
13	SGTC_1260	0700-1617	4.83	7.8 µM	3.52E-6	calcium & mitochondrial duress	17	6.82E-7
14	SGTC_255	tunicamycin	4.81	200.5 nM	3.87E-6	unfolded protein response	47	7.60E-7
15	SGTC_117	lucigenin	4.77	22.1 µM	4.66E-6	redox potentiating	64	9.42E-7
16	SGTC_2415	st077333	4.73	57.7 µM	5.43E-6		24	1.12E-6
17	SGTC_2360	9040895	4.72	200.0 µM	5.60E-6	RPP1 & pyrimidine depletion	11	1.16E-6
18	SGTC_2302	7771753	4.67	200.0 µM	7.12E-6		24	1.54E-6
19	SGTC_108	0509-0257	4.64	621.1 µM	8.07E-6		31	1.78E-6
20	SGTC_3157	9099274	4.63	49.5 µM	8.26E-6		12	1.82E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.319	8.66E-80	YNR006W	VPS27	hom	Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.318	2.13E-79	YKL041W	VPS24	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.309	5.44E-75	YPR173C	VPS4	hom	AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.253	6.27E-50	YDR495C	VPS3	hom	Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.236	8.48E-44	YDR323C	PEP7	hom	Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.211	4.16E-35	YKR035C_d	OPI8_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.209	1.86E-34	YOR036W	PEP12	hom	Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.207	8.06E-34	YKR035W-A	DID2	hom	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.190	9.88E-29	YPL084W	BRO1	hom	Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.190	1.69E-28	YDR486C	VPS60	hom	Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
0.184	7.51E-27	YPL065W	VPS28	hom	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.182	2.55E-26	YMR077C	VPS20	hom	Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.175	1.38E-24	YAL002W	VPS8	hom	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.170	4.90E-23	YGL212W	VAM7	hom	Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.159	2.08E-20	YOR089C	VPS21	hom	Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication