YKL004W / AUR1

Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL004W / AUR1

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:-3.51|P-value:1.30E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.52|P-value:9.09E-5 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.68|P-value:6.31E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.45|P-value:1.67E-6 ID:SGTC_640|Compound:3381-0960|FD-Score:5.43|P-value:4.26E-9 ID:SGTC_651|Compound:3253-0966|FD-Score:3.62|P-value:5.89E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:5.18|P-value:1.95E-8 ID:SGTC_669|Compound:0108-0021|FD-Score:-4.79|P-value:2.84E-7 ID:SGTC_723|Compound:4182-0001|FD-Score:3.87|P-value:1.98E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:4.13|P-value:5.68E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.75|P-value:3.53E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.09|P-value:6.46E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.27|P-value:2.51E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.96|P-value:7.95E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.26|P-value:3.11E-6 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.06|P-value:7.41E-4 ID:SGTC_1643|Compound:st009866|FD-Score:2.92|P-value:9.14E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.04|P-value:5.93E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.08|P-value:5.17E-4 ID:SGTC_1762|Compound:st045503|FD-Score:3.26|P-value:2.56E-4 ID:SGTC_1863|Compound:st057641|FD-Score:3.02|P-value:6.52E-4 ID:SGTC_2030|Compound:5175110|FD-Score:-3.64|P-value:7.31E-5 ID:SGTC_2032|Compound:5185112|FD-Score:-3.03|P-value:8.26E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.03|P-value:6.30E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.45|P-value:1.62E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-3.19|P-value:4.50E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.68|P-value:4.64E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:45.00|P-value:0 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.04|P-value:7.84E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.47|P-value:1.11E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.13|P-value:4.33E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.00|P-value:8.92E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.19|P-value:3.36E-4 ID:SGTC_2980|Compound:9014342|FD-Score:4.74|P-value:2.50E-7 ID:SGTC_2982|Compound:9019404|FD-Score:-4.70|P-value:4.62E-7 ID:SGTC_2984|Compound:9022109|FD-Score:4.79|P-value:1.84E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.33|P-value:2.65E-4 ID:SGTC_3160|Compound:9100216|FD-Score:2.96|P-value:7.92E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.09|P-value:6.58E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-5.29|P-value:1.59E-8 ID:SGTC_3309|Compound:9129340|FD-Score:2.93|P-value:9.10E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.51|P-value:1.30E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.52|P-value:9.09E-5 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.68|P-value:6.31E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.45|P-value:1.67E-6 ID:SGTC_640|Compound:3381-0960|FD-Score:5.43|P-value:4.26E-9 ID:SGTC_651|Compound:3253-0966|FD-Score:3.62|P-value:5.89E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:5.18|P-value:1.95E-8 ID:SGTC_669|Compound:0108-0021|FD-Score:-4.79|P-value:2.84E-7 ID:SGTC_723|Compound:4182-0001|FD-Score:3.87|P-value:1.98E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:4.13|P-value:5.68E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:-4.75|P-value:3.53E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.09|P-value:6.46E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.27|P-value:2.51E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.96|P-value:7.95E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:4.26|P-value:3.11E-6 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.06|P-value:7.41E-4 ID:SGTC_1643|Compound:st009866|FD-Score:2.92|P-value:9.14E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.04|P-value:5.93E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.08|P-value:5.17E-4 ID:SGTC_1762|Compound:st045503|FD-Score:3.26|P-value:2.56E-4 ID:SGTC_1863|Compound:st057641|FD-Score:3.02|P-value:6.52E-4 ID:SGTC_2030|Compound:5175110|FD-Score:-3.64|P-value:7.31E-5 ID:SGTC_2032|Compound:5185112|FD-Score:-3.03|P-value:8.26E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.03|P-value:6.30E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.45|P-value:1.62E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-3.19|P-value:4.50E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.68|P-value:4.64E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:45.00|P-value:0 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.04|P-value:7.84E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.47|P-value:1.11E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.13|P-value:4.33E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.00|P-value:8.92E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.19|P-value:3.36E-4 ID:SGTC_2980|Compound:9014342|FD-Score:4.74|P-value:2.50E-7 ID:SGTC_2982|Compound:9019404|FD-Score:-4.70|P-value:4.62E-7 ID:SGTC_2984|Compound:9022109|FD-Score:4.79|P-value:1.84E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.33|P-value:2.65E-4 ID:SGTC_3160|Compound:9100216|FD-Score:2.96|P-value:7.92E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.09|P-value:6.58E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-5.29|P-value:1.59E-8 ID:SGTC_3309|Compound:9129340|FD-Score:2.93|P-value:9.10E-4

Top fitness defect scores for YKL004W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2567 aureobasidin a 45.00 60.0 nM 0 2 0 10.60
2 SGTC_640 3381-0960 5.43 37.1 µM 4.26E-9 2 2.78E-8 0.81
3 SGTC_668 1682-0499 5.18 62.6 µM 1.95E-8 1 1.09E-7 0.61
4 SGTC_2984 9022109 4.79 39.0 µM 1.84E-7 2 8.21E-7 1.09
5 SGTC_2980 9014342 4.74 25.3 µM 2.50E-7 13 1.08E-6 0.01
6 SGTC_1381 2324-0066 4.26 62.1 µM 3.11E-6 8 1.04E-5 0.18
7 SGTC_870 0573-2213 4.13 57.6 µM 5.68E-6 ERG2 2 1.79E-5 0.75
8 SGTC_723 4182-0001 3.87 115.0 µM 1.98E-5 3 5.51E-5 0.02
9 SGTC_2318 6029776 3.68 164.0 µM 4.64E-5 6 1.18E-4 0.02
10 SGTC_651 3253-0966 3.62 34.4 µM 5.89E-5 calcium & mitochondrial duress 4 1.47E-4 0.29
11 SGTC_67 0335-0847 3.52 266.0 µM 9.09E-5 ubiquinone biosynthesis & proteasome 28 2.17E-4 0.05
12 SGTC_2794 7785382 3.47 27.3 µM 1.11E-4 19 2.59E-4 0.08
13 SGTC_1069 diphenylcyclopropenone 3.27 8.5 µM 2.51E-4 16 5.41E-4 0.07
14 SGTC_1762 st045503 3.26 25.5 µM 2.56E-4 6 5.51E-4 0.15
15 SGTC_2975 9084847 3.19 53.7 µM 3.36E-4 18 7.03E-4 0.16
16 SGTC_2800 7887935 3.13 81.8 µM 4.33E-4 RPP1 & pyrimidine depletion 19 8.83E-4 0.08
17 SGTC_1696 st025591 3.08 34.8 µM 5.17E-4 SWF1 & branched chain AA biosynthesis 12 0.00104 0.05
18 SGTC_1677 st019204 3.04 53.9 µM 5.93E-4 19 0.00117 0.22
19 SGTC_2293 7624732 3.03 200.0 µM 6.30E-4 17 0.00124 0.02
20 SGTC_1863 st057641 3.02 20.1 µM 6.52E-4 12 0.00128 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.540 2.60E-253 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.386 2.28E-119 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.360 2.33E-103 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.336 1.91E-89 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.310 1.66E-75 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.301 1.70E-71 YPL220W RPL1A hom Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.299 4.36E-70 YPR004C AIM45 hom Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response
0.265 6.04E-55 YGL169W SUA5 het Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
0.264 8.22E-55 YNL318C HXT14 hom Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.264 8.98E-55 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.261 2.93E-53 YDR224C HTB1 het Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.260 5.67E-53 YOR029W_d YOR029W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.255 1.10E-50 YBR056W YBR056W hom Putative glycoside hydrolase of the mitochondrial intermembrane space
0.249 1.12E-48 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
0.230 1.86E-41 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear