YKL039W / PTM1

Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL039W / PTM1

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-3.11|P-value:7.86E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.02|P-value:9.24E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.56|P-value:1.26E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.44|P-value:1.10E-14 ID:SGTC_263|Compound:alizarin|FD-Score:3.55|P-value:1.31E-4 ID:SGTC_276|Compound:colchicine|FD-Score:3.97|P-value:2.25E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:3.05|P-value:8.44E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.12|P-value:6.54E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.29|P-value:5.07E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:7.81|P-value:5.49E-16 ID:SGTC_637|Compound:1181-0519|FD-Score:5.80|P-value:1.25E-9 ID:SGTC_655|Compound:k060-0012|FD-Score:3.22|P-value:4.55E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.14|P-value:6.15E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.64|P-value:8.83E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.78|P-value:4.97E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:3.31|P-value:3.27E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.37|P-value:2.56E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.39|P-value:2.38E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.21|P-value:4.69E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.29|P-value:3.51E-4 ID:SGTC_1641|Compound:st009299|FD-Score:-3.06|P-value:9.38E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.37|P-value:2.61E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.30|P-value:3.42E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.96|P-value:2.33E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.52|P-value:1.70E-4 ID:SGTC_2276|Compound:7947513|FD-Score:3.97|P-value:2.19E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.25|P-value:4.04E-4 ID:SGTC_2345|Compound:9052315|FD-Score:4.44|P-value:2.54E-6 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.84|P-value:3.93E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:3.39|P-value:2.37E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.49|P-value:1.64E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.55|P-value:1.45E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-5.00|P-value:1.72E-7 ID:SGTC_2894|Compound:9048625|FD-Score:-3.77|P-value:6.26E-5 ID:SGTC_2955|Compound:9076254|FD-Score:4.21|P-value:7.63E-6 ID:SGTC_2959|Compound:9079155|FD-Score:4.50|P-value:1.87E-6 ID:SGTC_2981|Compound:9013952|FD-Score:-3.23|P-value:5.12E-4 ID:SGTC_3037|Compound:9091026|FD-Score:7.04|P-value:2.43E-13 ID:SGTC_3152|Compound:9099253|FD-Score:4.00|P-value:1.95E-5 ID:SGTC_3338|Compound:9144300|FD-Score:4.25|P-value:6.26E-6 ID:SGTC_3349|Compound:9149703|FD-Score:-4.10|P-value:1.47E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.30|P-value:3.33E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.11|P-value:7.86E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.02|P-value:9.24E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.56|P-value:1.26E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.44|P-value:1.10E-14 ID:SGTC_263|Compound:alizarin|FD-Score:3.55|P-value:1.31E-4 ID:SGTC_276|Compound:colchicine|FD-Score:3.97|P-value:2.25E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:3.05|P-value:8.44E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.12|P-value:6.54E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.29|P-value:5.07E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:7.81|P-value:5.49E-16 ID:SGTC_637|Compound:1181-0519|FD-Score:5.80|P-value:1.25E-9 ID:SGTC_655|Compound:k060-0012|FD-Score:3.22|P-value:4.55E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.14|P-value:6.15E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.64|P-value:8.83E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.78|P-value:4.97E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:3.31|P-value:3.27E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.37|P-value:2.56E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.39|P-value:2.38E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.21|P-value:4.69E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.29|P-value:3.51E-4 ID:SGTC_1641|Compound:st009299|FD-Score:-3.06|P-value:9.38E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.37|P-value:2.61E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.30|P-value:3.42E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.96|P-value:2.33E-5 ID:SGTC_2021|Compound:4019055|FD-Score:-3.52|P-value:1.70E-4 ID:SGTC_2276|Compound:7947513|FD-Score:3.97|P-value:2.19E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.25|P-value:4.04E-4 ID:SGTC_2345|Compound:9052315|FD-Score:4.44|P-value:2.54E-6 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.84|P-value:3.93E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:3.39|P-value:2.37E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.49|P-value:1.64E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.55|P-value:1.45E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-5.00|P-value:1.72E-7 ID:SGTC_2894|Compound:9048625|FD-Score:-3.77|P-value:6.26E-5 ID:SGTC_2955|Compound:9076254|FD-Score:4.21|P-value:7.63E-6 ID:SGTC_2959|Compound:9079155|FD-Score:4.50|P-value:1.87E-6 ID:SGTC_2981|Compound:9013952|FD-Score:-3.23|P-value:5.12E-4 ID:SGTC_3037|Compound:9091026|FD-Score:7.04|P-value:2.43E-13 ID:SGTC_3152|Compound:9099253|FD-Score:4.00|P-value:1.95E-5 ID:SGTC_3338|Compound:9144300|FD-Score:4.25|P-value:6.26E-6 ID:SGTC_3349|Compound:9149703|FD-Score:-4.10|P-value:1.47E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.30|P-value:3.33E-4

Top fitness defect scores for YKL039W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_568 1193-0097 7.81 7.5 µM 5.49E-16 ERAD & cell cycle 38 2.84E-15
2 SGTC_221 trifluoperazine 7.44 10.3 µM 1.10E-14 NEO1-PIK1 95 4.97E-14
3 SGTC_3037 9091026 7.04 49.5 µM 2.43E-13 NEO1-PIK1 26 9.43E-13
4 SGTC_637 1181-0519 5.80 7.3 µM 1.25E-9 fatty acid desaturase (OLE1) 56 3.22E-9
5 SGTC_2798 7813637 4.55 45.5 µM 1.45E-6 20 2.67E-6
6 SGTC_2959 9079155 4.50 11.0 µM 1.87E-6 16 3.41E-6
7 SGTC_2345 9052315 4.44 157.4 µM 2.54E-6 13 4.58E-6
8 SGTC_514 4466-0038 4.29 2.6 nM 5.07E-6 cell wall 136 8.84E-6
9 SGTC_3338 9144300 4.25 39.6 µM 6.26E-6 Golgi 25 1.08E-5
10 SGTC_2955 9076254 4.21 12.2 µM 7.63E-6 11 1.30E-5
11 SGTC_3152 9099253 4.00 49.5 µM 1.95E-5 NEO1-PIK1 126 3.18E-5
12 SGTC_2276 7947513 3.97 42.1 µM 2.19E-5 21 3.55E-5
13 SGTC_276 colchicine 3.97 750.0 µM 2.25E-5 18 3.65E-5
14 SGTC_2013 4024628 3.96 200.0 µM 2.33E-5 39 3.78E-5
15 SGTC_2538 3',7'-epoxycaryophyllan-6-one 3.84 100.0 µM 3.93E-5 25 6.21E-5
16 SGTC_964 1287-0107 3.78 19.4 µM 4.97E-5 cell wall 50 7.77E-5
17 SGTC_808 0711-0032 3.64 135.0 µM 8.83E-5 19 1.34E-4
18 SGTC_207 4185-0010 3.56 4.5 µM 1.26E-4 endomembrane recycling 110 1.88E-4
19 SGTC_263 alizarin 3.55 107.9 µM 1.31E-4 iron homeostasis 77 1.95E-4
20 SGTC_2796 7949634 3.49 45.5 µM 1.64E-4 36 2.42E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.353 2.70E-99 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.235 1.59E-43 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.218 2.19E-37 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.218 2.77E-37 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.206 1.44E-33 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.193 1.45E-29 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.186 2.45E-27 YOL159C-A YOL159C-A hom Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
0.184 8.84E-27 YNL295W_p YNL295W_p hom Putative protein of unknown function
0.182 1.75E-26 YIL002C INP51 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
0.182 2.20E-26 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.175 2.01E-24 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.173 5.04E-24 YLL036C PRP19 het Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
0.172 9.87E-24 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.165 7.09E-22 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.160 1.12E-20 YNL077W APJ1 hom Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress