YKL051W / SFK1

Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL051W / SFK1

Click on Significant Values for Screen Details ID:SGTC_310|Compound:1273-0058|FD-Score:2.57|P-value:7.63E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.95|P-value:5.46E-7 ID:SGTC_320|Compound:4092-0846|FD-Score:4.71|P-value:3.10E-9 ID:SGTC_327|Compound:1273-0057|FD-Score:2.61|P-value:6.46E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.78|P-value:1.77E-9 ID:SGTC_554|Compound:4483-2211|FD-Score:3.26|P-value:2.88E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:2.94|P-value:1.42E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:2.91|P-value:1.67E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.67|P-value:4.96E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.55|P-value:5.74E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:2.91|P-value:1.69E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:2.82|P-value:2.56E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.65|P-value:5.30E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.48|P-value:8.86E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.99|P-value:3.93E-7 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.02|P-value:9.75E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.90|P-value:6.97E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-2.75|P-value:3.30E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.88|P-value:1.90E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-2.55|P-value:8.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.45|P-value:9.64E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.10|P-value:6.63E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:2.61|P-value:6.53E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:2.80|P-value:2.81E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:2.57|P-value:7.59E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:2.59|P-value:6.85E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.17|P-value:8.79E-11 ID:SGTC_1673|Compound:st016611|FD-Score:2.82|P-value:2.52E-4 ID:SGTC_1712|Compound:st032288|FD-Score:12.50|P-value:1.99E-54 ID:SGTC_1746|Compound:st038117|FD-Score:2.72|P-value:4.00E-4 ID:SGTC_1906|Compound:st060206|FD-Score:2.74|P-value:3.57E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-2.85|P-value:2.06E-4 ID:SGTC_1911|Compound:st060208|FD-Score:2.67|P-value:5.04E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.15|P-value:5.16E-5 ID:SGTC_1992|Compound:st073643|FD-Score:-2.71|P-value:3.98E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.30|P-value:2.36E-5 ID:SGTC_2021|Compound:4019055|FD-Score:6.44|P-value:9.32E-16 ID:SGTC_2087|Compound:5276107|FD-Score:-2.50|P-value:9.80E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.40|P-value:1.34E-5 ID:SGTC_2107|Compound:5363541|FD-Score:2.69|P-value:4.42E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.25|P-value:3.05E-5 ID:SGTC_2316|Compound:5844804|FD-Score:3.27|P-value:2.74E-5 ID:SGTC_2359|Compound:9039622|FD-Score:3.58|P-value:4.86E-6 ID:SGTC_2367|Compound:9070478|FD-Score:4.71|P-value:3.04E-9 ID:SGTC_2372|Compound:9071633|FD-Score:2.69|P-value:4.58E-4 ID:SGTC_2438|Compound:5259590|FD-Score:2.62|P-value:6.05E-4 ID:SGTC_2451|Compound:5856223|FD-Score:-2.83|P-value:2.35E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.94|P-value:5.83E-7 ID:SGTC_2773|Compound:thioridazine|FD-Score:-2.52|P-value:9.28E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-2.53|P-value:8.80E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.78|P-value:3.05E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-2.60|P-value:6.45E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-2.74|P-value:3.43E-4 ID:SGTC_2896|Compound:9052656|FD-Score:6.15|P-value:1.60E-14 ID:SGTC_2984|Compound:9022109|FD-Score:-2.67|P-value:4.81E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.68|P-value:3.57E-9 ID:SGTC_3207|Compound:9114163|FD-Score:2.54|P-value:8.47E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.10|P-value:6.30E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:2.57|P-value:7.63E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.95|P-value:5.46E-7 ID:SGTC_320|Compound:4092-0846|FD-Score:4.71|P-value:3.10E-9 ID:SGTC_327|Compound:1273-0057|FD-Score:2.61|P-value:6.46E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.78|P-value:1.77E-9 ID:SGTC_554|Compound:4483-2211|FD-Score:3.26|P-value:2.88E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:2.94|P-value:1.42E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:2.91|P-value:1.67E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.67|P-value:4.96E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.55|P-value:5.74E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:2.91|P-value:1.69E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:2.82|P-value:2.56E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:2.65|P-value:5.30E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.48|P-value:8.86E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.99|P-value:3.93E-7 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.02|P-value:9.75E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.90|P-value:6.97E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-2.75|P-value:3.30E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.88|P-value:1.90E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-2.55|P-value:8.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.45|P-value:9.64E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.10|P-value:6.63E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:2.61|P-value:6.53E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:2.80|P-value:2.81E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:2.57|P-value:7.59E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:2.59|P-value:6.85E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.17|P-value:8.79E-11 ID:SGTC_1673|Compound:st016611|FD-Score:2.82|P-value:2.52E-4 ID:SGTC_1712|Compound:st032288|FD-Score:12.50|P-value:1.99E-54 ID:SGTC_1746|Compound:st038117|FD-Score:2.72|P-value:4.00E-4 ID:SGTC_1906|Compound:st060206|FD-Score:2.74|P-value:3.57E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-2.85|P-value:2.06E-4 ID:SGTC_1911|Compound:st060208|FD-Score:2.67|P-value:5.04E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.15|P-value:5.16E-5 ID:SGTC_1992|Compound:st073643|FD-Score:-2.71|P-value:3.98E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.30|P-value:2.36E-5 ID:SGTC_2021|Compound:4019055|FD-Score:6.44|P-value:9.32E-16 ID:SGTC_2087|Compound:5276107|FD-Score:-2.50|P-value:9.80E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.40|P-value:1.34E-5 ID:SGTC_2107|Compound:5363541|FD-Score:2.69|P-value:4.42E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.25|P-value:3.05E-5 ID:SGTC_2316|Compound:5844804|FD-Score:3.27|P-value:2.74E-5 ID:SGTC_2359|Compound:9039622|FD-Score:3.58|P-value:4.86E-6 ID:SGTC_2367|Compound:9070478|FD-Score:4.71|P-value:3.04E-9 ID:SGTC_2372|Compound:9071633|FD-Score:2.69|P-value:4.58E-4 ID:SGTC_2438|Compound:5259590|FD-Score:2.62|P-value:6.05E-4 ID:SGTC_2451|Compound:5856223|FD-Score:-2.83|P-value:2.35E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.94|P-value:5.83E-7 ID:SGTC_2773|Compound:thioridazine|FD-Score:-2.52|P-value:9.28E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-2.53|P-value:8.80E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.78|P-value:3.05E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-2.60|P-value:6.45E-4 ID:SGTC_2869|Compound:9037858|FD-Score:-2.74|P-value:3.43E-4 ID:SGTC_2896|Compound:9052656|FD-Score:6.15|P-value:1.60E-14 ID:SGTC_2984|Compound:9022109|FD-Score:-2.67|P-value:4.81E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.68|P-value:3.57E-9 ID:SGTC_3207|Compound:9114163|FD-Score:2.54|P-value:8.47E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.10|P-value:6.30E-5

Top fitness defect scores for YKL051W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1712 st032288 12.50 53.4 µM 1.99E-54 calcium & mitochondrial duress 7 2.22E-36
2 SGTC_2021 4019055 6.44 173.0 µM 9.32E-16 endomembrane recycling 33 6.05E-11
3 SGTC_2896 9052656 6.15 71.4 µM 1.60E-14 endomembrane recycling 37 3.96E-10
4 SGTC_1597 7-benzyloxygramine 5.17 47.5 µM 8.79E-11 calcium & mitochondrial duress 63 1.18E-7
5 SGTC_528 3492-0019 4.78 172.0 µM 1.77E-9 calcium & mitochondrial duress 165 8.67E-7
6 SGTC_2367 9070478 4.71 135.4 µM 3.04E-9 cell wall 23 1.24E-6
7 SGTC_320 4092-0846 4.71 5.3 µM 3.10E-9 ergosterol depletion effects on membrane 31 1.26E-6
8 SGTC_312 4092-0653 3.95 6.8 µM 5.46E-7 ergosterol depletion effects on membrane 85 3.93E-5
9 SGTC_2688 kanamycin b 3.94 96.7 µM 5.83E-7 calcium & mitochondrial duress 367 4.10E-5
10 SGTC_2359 9039622 3.58 200.0 µM 4.86E-6 endomembrane recycling 30 1.69E-4
11 SGTC_940 3232-1287 3.55 54.8 µM 5.74E-6 NEO1 53 1.89E-4
12 SGTC_1083 didemethylchlorpromazine sulfoxide 3.48 707.4 nM 8.86E-6 redox potentiating 106 2.53E-4
13 SGTC_2103 5378138 3.40 10.0 µM 1.34E-5 35 3.35E-4
14 SGTC_2014 4013930 3.30 200.0 µM 2.36E-5 68 4.88E-4
15 SGTC_2316 5844804 3.27 197.4 µM 2.74E-5 69 5.40E-4
16 SGTC_554 4483-2211 3.26 187.0 µM 2.88E-5 37 5.58E-4
17 SGTC_2121 5257820 3.25 149.6 µM 3.05E-5 25 5.81E-4
18 SGTC_1979 st071809 3.15 45.3 µM 5.16E-5 RPP1 & pyrimidine depletion 45 8.26E-4
19 SGTC_1310 1188-0297 3.10 994.0 nM 6.63E-5 azole & statin 68 9.79E-4
20 SGTC_1122 capsaicin 3.02 818.6 nM 9.75E-5 68 0.00127

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.405 1.89E-132 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.399 1.16E-128 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.377 7.20E-114 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.377 1.77E-113 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.372 4.58E-110 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.361 3.61E-104 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.359 2.12E-102 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.313 4.94E-77 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.305 2.17E-73 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.303 5.00E-72 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.293 2.41E-67 YGL151W NUT1 hom Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
0.284 4.06E-63 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.283 5.82E-63 YCL021W-A_p YCL021W-A_p hom Putative protein of unknown function
0.280 1.16E-61 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.280 2.75E-61 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication