YKL058W / TOA2

TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL058W / TOA2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.81|P-value:1.95E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:3.98|P-value:1.04E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.40|P-value:6.75E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.59|P-value:3.60E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:13.00|P-value:1.72E-34 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.36|P-value:8.74E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-4.74|P-value:4.13E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:4.63|P-value:7.83E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.69|P-value:2.92E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:6.10|P-value:6.47E-9 ID:SGTC_187|Compound:1488-2104|FD-Score:3.57|P-value:4.42E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.26|P-value:4.60E-7 ID:SGTC_298|Compound:1391-0729|FD-Score:8.13|P-value:1.64E-14 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.29|P-value:9.67E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.56|P-value:4.57E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.09|P-value:6.60E-9 ID:SGTC_352|Compound:k048-0007|FD-Score:3.75|P-value:2.41E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.66|P-value:3.25E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:8.02|P-value:3.41E-14 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.86|P-value:1.42E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.68|P-value:6.33E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:4.42|P-value:1.90E-5 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.97|P-value:9.24E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:5.14|P-value:8.05E-7 ID:SGTC_660|Compound:4031-1742|FD-Score:5.94|P-value:1.51E-8 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:4.78E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.92|P-value:2.25E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.81|P-value:1.95E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.42|P-value:7.32E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:9.43|P-value:6.58E-19 ID:SGTC_939|Compound:1165-0479|FD-Score:3.84|P-value:1.77E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.51|P-value:4.70E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.75|P-value:2.36E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:8.37|P-value:2.81E-15 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.86|P-value:1.64E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.69|P-value:2.41E-16 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.15|P-value:5.50E-5 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.56|P-value:4.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.08|P-value:1.08E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.00|P-value:9.61E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.78|P-value:4.24E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.45|P-value:6.54E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:17.20|P-value:2.32E-58 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.37|P-value:8.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.47|P-value:1.57E-5 ID:SGTC_1780|Compound:st038448|FD-Score:3.52|P-value:5.29E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-4.61|P-value:7.34E-6 ID:SGTC_2021|Compound:4019055|FD-Score:6.41|P-value:1.12E-9 ID:SGTC_2036|Compound:5160441|FD-Score:9.11|P-value:8.84E-18 ID:SGTC_2038|Compound:4100038|FD-Score:4.70|P-value:5.87E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-3.65|P-value:2.96E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.73|P-value:2.57E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:6.24|P-value:2.87E-9 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.68|P-value:6.56E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.91|P-value:1.34E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.57|P-value:4.40E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.55|P-value:4.74E-4 ID:SGTC_2784|Compound:7567673|FD-Score:5.27|P-value:4.51E-7 ID:SGTC_2873|Compound:9040596|FD-Score:3.37|P-value:8.44E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.64|P-value:3.04E-4 ID:SGTC_2915|Compound:7971052|FD-Score:3.44|P-value:6.86E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_2973|Compound:9094665|FD-Score:5.11|P-value:9.54E-7 ID:SGTC_2974|Compound:9094440|FD-Score:4.22|P-value:4.20E-5 ID:SGTC_2991|Compound:9074669|FD-Score:5.98|P-value:1.24E-8 ID:SGTC_3018|Compound:9082602|FD-Score:4.31|P-value:2.94E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.36|P-value:7.80E-4 ID:SGTC_3120|Compound:9124077|FD-Score:9.51|P-value:3.30E-19 ID:SGTC_3150|Compound:9097574|FD-Score:3.81|P-value:1.97E-4 ID:SGTC_3152|Compound:9099253|FD-Score:11.80|P-value:8.88E-29 ID:SGTC_3219|Compound:9131112|FD-Score:5.26|P-value:4.78E-7 ID:SGTC_3288|Compound:9119690|FD-Score:6.29|P-value:2.21E-9 ID:SGTC_3300|Compound:9124615|FD-Score:-3.58|P-value:3.78E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.31|P-value:9.01E-4 ID:SGTC_3304|Compound:9125678|FD-Score:38.40|P-value:6.98E-283 ID:SGTC_15|Compound:0266-0086|FD-Score:3.81|P-value:1.95E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:3.98|P-value:1.04E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.40|P-value:6.75E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.59|P-value:3.60E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:13.00|P-value:1.72E-34 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.36|P-value:8.74E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-4.74|P-value:4.13E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:4.63|P-value:7.83E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.69|P-value:2.92E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:6.10|P-value:6.47E-9 ID:SGTC_187|Compound:1488-2104|FD-Score:3.57|P-value:4.42E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.26|P-value:4.60E-7 ID:SGTC_298|Compound:1391-0729|FD-Score:8.13|P-value:1.64E-14 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.29|P-value:9.67E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.56|P-value:4.57E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.09|P-value:6.60E-9 ID:SGTC_352|Compound:k048-0007|FD-Score:3.75|P-value:2.41E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.66|P-value:3.25E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:8.02|P-value:3.41E-14 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.86|P-value:1.42E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.68|P-value:6.33E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:4.42|P-value:1.90E-5 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.97|P-value:9.24E-5 ID:SGTC_614|Compound:0462-0418|FD-Score:5.14|P-value:8.05E-7 ID:SGTC_660|Compound:4031-1742|FD-Score:5.94|P-value:1.51E-8 ID:SGTC_796|Compound:1234-0020|FD-Score:3.55|P-value:4.78E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.92|P-value:2.25E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.81|P-value:1.95E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.42|P-value:7.32E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:9.43|P-value:6.58E-19 ID:SGTC_939|Compound:1165-0479|FD-Score:3.84|P-value:1.77E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.51|P-value:4.70E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.75|P-value:2.36E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:8.37|P-value:2.81E-15 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.86|P-value:1.64E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:8.69|P-value:2.41E-16 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.15|P-value:5.50E-5 ID:SGTC_1211|Compound:1056-0033|FD-Score:3.56|P-value:4.63E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.08|P-value:1.08E-6 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.00|P-value:9.61E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.78|P-value:4.24E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.45|P-value:6.54E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:17.20|P-value:2.32E-58 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.37|P-value:8.55E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.47|P-value:1.57E-5 ID:SGTC_1780|Compound:st038448|FD-Score:3.52|P-value:5.29E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-4.61|P-value:7.34E-6 ID:SGTC_2021|Compound:4019055|FD-Score:6.41|P-value:1.12E-9 ID:SGTC_2036|Compound:5160441|FD-Score:9.11|P-value:8.84E-18 ID:SGTC_2038|Compound:4100038|FD-Score:4.70|P-value:5.87E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-3.65|P-value:2.96E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.73|P-value:2.57E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:6.24|P-value:2.87E-9 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.68|P-value:6.56E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.91|P-value:1.34E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.57|P-value:4.40E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.55|P-value:4.74E-4 ID:SGTC_2784|Compound:7567673|FD-Score:5.27|P-value:4.51E-7 ID:SGTC_2873|Compound:9040596|FD-Score:3.37|P-value:8.44E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.64|P-value:3.04E-4 ID:SGTC_2915|Compound:7971052|FD-Score:3.44|P-value:6.86E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.96|P-value:1.12E-4 ID:SGTC_2973|Compound:9094665|FD-Score:5.11|P-value:9.54E-7 ID:SGTC_2974|Compound:9094440|FD-Score:4.22|P-value:4.20E-5 ID:SGTC_2991|Compound:9074669|FD-Score:5.98|P-value:1.24E-8 ID:SGTC_3018|Compound:9082602|FD-Score:4.31|P-value:2.94E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.36|P-value:7.80E-4 ID:SGTC_3120|Compound:9124077|FD-Score:9.51|P-value:3.30E-19 ID:SGTC_3150|Compound:9097574|FD-Score:3.81|P-value:1.97E-4 ID:SGTC_3152|Compound:9099253|FD-Score:11.80|P-value:8.88E-29 ID:SGTC_3219|Compound:9131112|FD-Score:5.26|P-value:4.78E-7 ID:SGTC_3288|Compound:9119690|FD-Score:6.29|P-value:2.21E-9 ID:SGTC_3300|Compound:9124615|FD-Score:-3.58|P-value:3.78E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.31|P-value:9.01E-4 ID:SGTC_3304|Compound:9125678|FD-Score:38.40|P-value:6.98E-283

Top fitness defect scores for YKL058W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3304 9125678 38.40 46.1 µM 6.98E-283 3 0 10.90
2 SGTC_1597 7-benzyloxygramine 17.20 47.5 µM 2.32E-58 calcium & mitochondrial duress 3 1.83E-66 0.19
3 SGTC_39 0986-0283 13.00 58.4 µM 1.72E-34 calcium & mitochondrial duress 3 3.38E-39 6.38
4 SGTC_3152 9099253 11.80 49.5 µM 8.88E-29 NEO1-PIK1 4 1.13E-32 2.31
5 SGTC_3120 9124077 9.51 49.5 µM 3.30E-19 7 9.65E-22 1.60
6 SGTC_902 1120-0002 9.43 2.1 µM 6.58E-19 azole & statin 1 2.12E-21 2.22
7 SGTC_2036 5160441 9.11 105.0 µM 8.84E-18 NEO1 6 4.13E-20 0.32
8 SGTC_1161 k007-0610 8.69 110.0 µM 2.41E-16 ERAD & cell cycle 7 1.80E-18 0.48
9 SGTC_1057 3448-5381 8.37 134.0 µM 2.81E-15 calcium & mitochondrial duress 3 2.98E-17 1.52
10 SGTC_298 1391-0729 8.13 10.0 µM 1.64E-14 calcium & mitochondrial duress 1 2.23E-16 2.76
11 SGTC_467 ro 31-8220 8.02 72.8 µM 3.41E-14 calcium & mitochondrial duress 3 5.14E-16 0.82
12 SGTC_2021 4019055 6.41 173.0 µM 1.12E-9 endomembrane recycling 17 7.37E-11 0.28
13 SGTC_3288 9119690 6.29 36.2 µM 2.21E-9 10 1.60E-10 0.47
14 SGTC_2408 sulbentine 6.24 18.4 µM 2.87E-9 18 2.15E-10 0.06
15 SGTC_120 rhodamine 6G 6.10 52.3 µM 6.47E-9 mitochondrial processes 5 5.45E-10 0.36
16 SGTC_351 caspofungin 6.09 25.0 nM 6.60E-9 cell wall 12 5.58E-10 0.07
17 SGTC_2991 9074669 5.98 71.4 µM 1.24E-8 azole & statin 5 1.14E-9 0.11
18 SGTC_660 4031-1742 5.94 102.0 µM 1.51E-8 redox potentiating 3 1.44E-9 0.78
19 SGTC_2784 7567673 5.27 72.7 µM 4.51E-7 iron homeostasis 5 6.91E-8 0.07
20 SGTC_197 4544-0043 5.26 41.6 µM 4.60E-7 endomembrane recycling 14 7.07E-8 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.546 4.84E-260 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.537 5.92E-250 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.388 8.53E-121 YLR229C CDC42 het Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
0.377 9.44E-114 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.365 2.68E-106 YLR153C ACS2 het Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
0.321 5.58E-81 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.260 7.89E-53 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.224 3.00E-39 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.202 2.93E-32 YML017W PSP2 hom Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
0.201 5.13E-32 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.190 1.70E-28 YER133W GLC7 het Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
0.188 4.76E-28 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.187 1.09E-27 YGL049C TIF4632 hom Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
0.182 2.57E-26 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.178 3.89E-25 YIL154C IMP2' hom Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat