YKL059C / MPE1

Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL059C / MPE1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.29|P-value:4.16E-6 ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_33|Compound:0986-0033|FD-Score:3.18|P-value:4.96E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:3.11|P-value:6.39E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:4.45|P-value:1.91E-6 ID:SGTC_196|Compound:k015-0023|FD-Score:3.46|P-value:1.66E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:6.29|P-value:2.95E-11 ID:SGTC_325|Compound:2810-4341|FD-Score:4.32|P-value:3.56E-6 ID:SGTC_326|Compound:k292-0812|FD-Score:6.62|P-value:2.77E-12 ID:SGTC_327|Compound:1273-0057|FD-Score:3.25|P-value:3.75E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:3.72|P-value:5.71E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:4.14|P-value:8.76E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.40|P-value:2.12E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.42|P-value:1.58E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.06|P-value:7.73E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.10|P-value:6.52E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.70|P-value:6.11E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.32|P-value:2.78E-6 ID:SGTC_1201|Compound:1889-3372|FD-Score:2.99|P-value:9.64E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.21|P-value:4.48E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.65|P-value:7.71E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-3.12|P-value:5.15E-4 ID:SGTC_1805|Compound:st050013|FD-Score:3.29|P-value:3.26E-4 ID:SGTC_1822|Compound:st052468|FD-Score:-3.30|P-value:2.52E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.23|P-value:3.30E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.85|P-value:2.35E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.40|P-value:2.16E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.86|P-value:3.10E-5 ID:SGTC_2036|Compound:5160441|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_2105|Compound:5350118|FD-Score:-3.09|P-value:5.67E-4 ID:SGTC_2362|Compound:9071156|FD-Score:3.31|P-value:3.04E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.16|P-value:4.35E-4 ID:SGTC_2388|Compound:9080168|FD-Score:-3.93|P-value:1.80E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.45|P-value:1.71E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-2.94|P-value:9.81E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.49|P-value:1.20E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.76|P-value:3.81E-5 ID:SGTC_2868|Compound:9035608|FD-Score:-3.69|P-value:5.12E-5 ID:SGTC_2946|Compound:9065502|FD-Score:3.80|P-value:4.06E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.50|P-value:1.47E-6 ID:SGTC_3096|Compound:9117468|FD-Score:3.15|P-value:5.55E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.48|P-value:1.65E-6 ID:SGTC_10|Compound:0279-0212|FD-Score:4.29|P-value:4.16E-6 ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:4.38|P-value:2.70E-6 ID:SGTC_33|Compound:0986-0033|FD-Score:3.18|P-value:4.96E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:3.11|P-value:6.39E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:4.45|P-value:1.91E-6 ID:SGTC_196|Compound:k015-0023|FD-Score:3.46|P-value:1.66E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:6.29|P-value:2.95E-11 ID:SGTC_325|Compound:2810-4341|FD-Score:4.32|P-value:3.56E-6 ID:SGTC_326|Compound:k292-0812|FD-Score:6.62|P-value:2.77E-12 ID:SGTC_327|Compound:1273-0057|FD-Score:3.25|P-value:3.75E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:3.72|P-value:5.71E-5 ID:SGTC_382|Compound:3229-0966|FD-Score:4.14|P-value:8.76E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.40|P-value:2.12E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.42|P-value:1.58E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.06|P-value:7.73E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.10|P-value:6.52E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.70|P-value:6.11E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.32|P-value:2.78E-6 ID:SGTC_1201|Compound:1889-3372|FD-Score:2.99|P-value:9.64E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.21|P-value:4.48E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.65|P-value:7.71E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-3.12|P-value:5.15E-4 ID:SGTC_1805|Compound:st050013|FD-Score:3.29|P-value:3.26E-4 ID:SGTC_1822|Compound:st052468|FD-Score:-3.30|P-value:2.52E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-3.23|P-value:3.30E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.85|P-value:2.35E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.40|P-value:2.16E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.86|P-value:3.10E-5 ID:SGTC_2036|Compound:5160441|FD-Score:3.12|P-value:6.06E-4 ID:SGTC_2105|Compound:5350118|FD-Score:-3.09|P-value:5.67E-4 ID:SGTC_2362|Compound:9071156|FD-Score:3.31|P-value:3.04E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.16|P-value:4.35E-4 ID:SGTC_2388|Compound:9080168|FD-Score:-3.93|P-value:1.80E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.45|P-value:1.71E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-2.94|P-value:9.81E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.49|P-value:1.20E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.76|P-value:3.81E-5 ID:SGTC_2868|Compound:9035608|FD-Score:-3.69|P-value:5.12E-5 ID:SGTC_2946|Compound:9065502|FD-Score:3.80|P-value:4.06E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.50|P-value:1.47E-6 ID:SGTC_3096|Compound:9117468|FD-Score:3.15|P-value:5.55E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.48|P-value:1.65E-6

Top fitness defect scores for YKL059C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_326 k292-0812 6.62 17.5 µM 2.77E-12 1 1.77E-11 2.61
2 SGTC_323 4013-0906 6.29 34.3 µM 2.95E-11 ERG2 1 1.56E-10 2.04
3 SGTC_1968 st069590 4.85 43.6 µM 2.35E-7 11 6.22E-7 0.06
4 SGTC_2970 9090938 4.50 41.9 µM 1.47E-6 RNA processing & uracil transport 8 3.38E-6 0.16
5 SGTC_3308 9128332 4.48 17.6 µM 1.65E-6 plasma membrane duress 7 3.76E-6 0.23
6 SGTC_130 0136-0219 4.45 337.7 µM 1.91E-6 6 4.31E-6 0.02
7 SGTC_30 5-(2-thienylidene)-Rhodanine 4.38 28.7 µM 2.70E-6 2 5.92E-6 0.69
8 SGTC_325 2810-4341 4.32 181.0 µM 3.56E-6 amide catabolism 3 7.65E-6 0.16
9 SGTC_10 0279-0212 4.29 42.6 µM 4.16E-6 TRP & mitochondrial translation 6 8.85E-6 0.26
10 SGTC_382 3229-0966 4.14 23.2 µM 8.76E-6 4 1.76E-5 0.14
11 SGTC_2025 5147754 3.86 141.0 µM 3.10E-5 7 5.68E-5 0.15
12 SGTC_2946 9065502 3.80 71.4 µM 4.06E-5 2 7.28E-5 0.27
13 SGTC_328 k915-0120 3.72 63.5 µM 5.71E-5 TSC3-RPN4 4 9.99E-5 0.09
14 SGTC_863 0466-0191 3.70 129.0 µM 6.11E-5 cell wall 21 1.06E-4 0.41
15 SGTC_1649 st011737 3.65 51.3 µM 7.71E-5 3 1.32E-4 0.33
16 SGTC_196 k015-0023 3.46 18.1 µM 1.66E-4 PDR1 3 2.68E-4 0.07
17 SGTC_2574 2',4'-dihydroxychalcone 3.45 14.6 µM 1.71E-4 19 2.77E-4 0.05
18 SGTC_439 amiodarone 3.40 34.4 µM 2.12E-4 NEO1-PIK1 10 3.36E-4 0.01
19 SGTC_1983 st071813 3.40 46.9 µM 2.16E-4 RPP1 & pyrimidine depletion 9 3.43E-4 0.23
20 SGTC_2362 9071156 3.31 126.0 µM 3.04E-4 9 4.70E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 3.17E-16 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.135 4.03E-15 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.130 4.64E-14 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.127 1.41E-13 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.125 3.59E-13 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.120 2.99E-12 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.118 5.67E-12 YBR263W SHM1 hom Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
0.117 1.15E-11 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.114 3.40E-11 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.107 5.68E-10 YNR067C DSE4 hom Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother
0.106 7.43E-10 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.103 2.24E-9 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.102 2.65E-9 YDR110W FOB1 hom Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases
0.095 3.62E-8 YER071C_p TDA2_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele
0.094 4.22E-8 YBR276C PPS1 hom Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle