YKL067W / YNK1

Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL067W / YNK1

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:2.78|P-value:7.43E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-2.80|P-value:7.24E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-2.74|P-value:9.09E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.33|P-value:6.95E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:4.24|P-value:6.27E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.16|P-value:1.59E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.72|P-value:1.11E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:2.78|P-value:7.31E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.20|P-value:1.28E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.56|P-value:2.33E-5 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.28|P-value:8.87E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:2.71|P-value:9.85E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.52|P-value:2.91E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.75|P-value:8.41E-4 ID:SGTC_1567|Compound:st069303|FD-Score:2.88|P-value:5.03E-4 ID:SGTC_1626|Compound:st003712|FD-Score:4.16|P-value:1.01E-6 ID:SGTC_1865|Compound:st057639|FD-Score:-3.82|P-value:6.57E-6 ID:SGTC_1870|Compound:st057514|FD-Score:-3.48|P-value:3.60E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-2.71|P-value:9.97E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.18|P-value:1.39E-4 ID:SGTC_2163|Compound:5671233|FD-Score:2.79|P-value:7.16E-4 ID:SGTC_2348|Compound:9022543|FD-Score:2.80|P-value:6.72E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.08|P-value:2.16E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.83|P-value:6.05E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.56|P-value:2.46E-5 ID:SGTC_2800|Compound:7887935|FD-Score:-2.73|P-value:9.40E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.07|P-value:2.40E-4 ID:SGTC_2919|Compound:7978188|FD-Score:5.87|P-value:1.04E-11 ID:SGTC_3064|Compound:1327-0027|FD-Score:2.80|P-value:6.91E-4 ID:SGTC_3153|Compound:9100258|FD-Score:4.09|P-value:1.50E-6 ID:SGTC_3300|Compound:9124615|FD-Score:3.80|P-value:6.97E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.15|P-value:1.67E-4 ID:SGTC_3308|Compound:9128332|FD-Score:2.97|P-value:3.43E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-2.94|P-value:4.01E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-2.98|P-value:3.38E-4 ID:SGTC_3327|Compound:9140648|FD-Score:2.85|P-value:5.65E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:2.78|P-value:7.43E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-2.80|P-value:7.24E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-2.74|P-value:9.09E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.33|P-value:6.95E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:4.24|P-value:6.27E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.16|P-value:1.59E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.72|P-value:1.11E-5 ID:SGTC_935|Compound:2996-0153|FD-Score:2.78|P-value:7.31E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.20|P-value:1.28E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.56|P-value:2.33E-5 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.28|P-value:8.87E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:2.71|P-value:9.85E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.52|P-value:2.91E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.75|P-value:8.41E-4 ID:SGTC_1567|Compound:st069303|FD-Score:2.88|P-value:5.03E-4 ID:SGTC_1626|Compound:st003712|FD-Score:4.16|P-value:1.01E-6 ID:SGTC_1865|Compound:st057639|FD-Score:-3.82|P-value:6.57E-6 ID:SGTC_1870|Compound:st057514|FD-Score:-3.48|P-value:3.60E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-2.71|P-value:9.97E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.18|P-value:1.39E-4 ID:SGTC_2163|Compound:5671233|FD-Score:2.79|P-value:7.16E-4 ID:SGTC_2348|Compound:9022543|FD-Score:2.80|P-value:6.72E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:3.08|P-value:2.16E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:2.83|P-value:6.05E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.56|P-value:2.46E-5 ID:SGTC_2800|Compound:7887935|FD-Score:-2.73|P-value:9.40E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.07|P-value:2.40E-4 ID:SGTC_2919|Compound:7978188|FD-Score:5.87|P-value:1.04E-11 ID:SGTC_3064|Compound:1327-0027|FD-Score:2.80|P-value:6.91E-4 ID:SGTC_3153|Compound:9100258|FD-Score:4.09|P-value:1.50E-6 ID:SGTC_3300|Compound:9124615|FD-Score:3.80|P-value:6.97E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.15|P-value:1.67E-4 ID:SGTC_3308|Compound:9128332|FD-Score:2.97|P-value:3.43E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-2.94|P-value:4.01E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-2.98|P-value:3.38E-4 ID:SGTC_3327|Compound:9140648|FD-Score:2.85|P-value:5.65E-4

Top fitness defect scores for YKL067W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2919 7978188 5.87 46.4 µM 1.04E-11 4 2.22E-9
2 SGTC_346 4513-0042 4.24 7.2 µM 6.27E-7 azole & statin 34 1.10E-5
3 SGTC_1626 st003712 4.16 1.8 µM 1.01E-6 19 1.60E-5
4 SGTC_3153 9100258 4.09 49.5 µM 1.50E-6 15 2.17E-5
5 SGTC_3300 9124615 3.80 50.5 µM 6.97E-6 21 7.16E-5
6 SGTC_1113 artemisinin 3.56 1.2 µM 2.33E-5 heme biosynthesis & mitochondrial translocase 23 1.83E-4
7 SGTC_1311 1190-0148 3.52 100.0 µM 2.91E-5 azole & statin 39 2.18E-4
8 SGTC_185 k048-0037 3.33 25.5 µM 6.95E-5 RSC & ERG11 23 4.29E-4
9 SGTC_1142 3253-1408 3.28 9.8 µM 8.87E-5 25 5.18E-4
10 SGTC_968 1309-0633 3.20 340.0 µM 1.28E-4 34 6.88E-4
11 SGTC_2012 4023501 3.18 200.0 µM 1.39E-4 64 7.35E-4
12 SGTC_2586 curcumin 3.08 80.0 µM 2.16E-4 iron homeostasis 104 0.00104
13 SGTC_3308 9128332 2.97 17.6 µM 3.43E-4 plasma membrane duress 145 0.00149
14 SGTC_1567 st069303 2.88 68.4 µM 5.03E-4 mitochondrial processes 85 0.00201
15 SGTC_3327 9140648 2.85 26.9 µM 5.65E-4 49 0.00220
16 SGTC_2588 indole-3-carbinol 2.83 100.0 µM 6.05E-4 122 0.00232
17 SGTC_2348 9022543 2.80 33.4 µM 6.72E-4 mitochondrial processes 79 0.00252
18 SGTC_3064 1327-0027 2.80 10.0 µM 6.91E-4 31 0.00257
19 SGTC_2163 5671233 2.79 125.0 µM 7.16E-4 92 0.00265
20 SGTC_935 2996-0153 2.78 430.0 µM 7.31E-4 PDR1 81 0.00269

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 3.99E-19 YOR059C YOR059C hom Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
0.134 7.72E-15 YPL116W HOS3 hom Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
0.133 1.21E-14 YHR175W-A_p YHR175W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.122 1.23E-12 YBR060C ORC2 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
0.116 1.89E-11 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.112 8.71E-11 YNL009W IDP3 hom Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
0.109 2.20E-10 YKR054C DYN1 hom Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p
0.107 4.53E-10 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.102 3.34E-9 YOR365C_p YOR365C_p hom Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
0.100 6.71E-9 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.099 9.99E-9 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.096 2.63E-8 YMR158W-B_d YMR158W-B_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C
0.095 3.44E-8 YBR054W YRO2 hom Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
0.093 7.22E-8 YBR161W CSH1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
0.087 4.50E-7 YPL159C PET20 hom Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome