YKL077W_p

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL077W / YKL077W

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.88|P-value:1.28E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.51|P-value:1.10E-7 ID:SGTC_38|Compound:0929-0051|FD-Score:3.29|P-value:9.83E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:4.05|P-value:7.01E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:12.00|P-value:1.28E-29 ID:SGTC_67|Compound:0335-0847|FD-Score:5.84|P-value:1.97E-8 ID:SGTC_86|Compound:0417-1630|FD-Score:7.80|P-value:1.09E-13 ID:SGTC_142|Compound:0335-0849|FD-Score:8.01|P-value:2.44E-14 ID:SGTC_153|Compound:4077-0274|FD-Score:3.61|P-value:3.39E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.98|P-value:9.14E-5 ID:SGTC_200|Compound:k038-0079|FD-Score:3.49|P-value:5.09E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:8.08|P-value:1.55E-14 ID:SGTC_206|Compound:4546-0025|FD-Score:5.80|P-value:2.48E-8 ID:SGTC_207|Compound:4185-0010|FD-Score:3.45|P-value:5.77E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.75|P-value:3.95E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:8.09|P-value:1.44E-14 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.47|P-value:1.38E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:9.60|P-value:8.58E-20 ID:SGTC_577|Compound:r071-0003|FD-Score:3.47|P-value:5.39E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:5.30|P-value:3.09E-7 ID:SGTC_786|Compound:4544-0069|FD-Score:7.07|P-value:1.49E-11 ID:SGTC_788|Compound:1182-0090|FD-Score:5.11|P-value:7.62E-7 ID:SGTC_819|Compound:1013-0258|FD-Score:8.00|P-value:2.60E-14 ID:SGTC_841|Compound:0251-0215|FD-Score:6.99|P-value:2.50E-11 ID:SGTC_892|Compound:0929-0076|FD-Score:3.84|P-value:1.53E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.61|P-value:3.36E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.44|P-value:6.08E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.59|P-value:3.81E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.32|P-value:9.29E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.33|P-value:8.65E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.80|P-value:1.76E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.97|P-value:9.58E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.80|P-value:1.75E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.86|P-value:1.42E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-3.42|P-value:6.57E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.50|P-value:4.95E-10 ID:SGTC_1687|Compound:st020778|FD-Score:11.20|P-value:4.05E-26 ID:SGTC_1694|Compound:st024011|FD-Score:4.01|P-value:7.95E-5 ID:SGTC_1729|Compound:st035615|FD-Score:4.12|P-value:5.31E-5 ID:SGTC_1736|Compound:st036774|FD-Score:3.45|P-value:5.82E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.32|P-value:8.86E-4 ID:SGTC_1881|Compound:5652484|FD-Score:5.34|P-value:2.59E-7 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.89|P-value:1.25E-4 ID:SGTC_1921|Compound:5740136|FD-Score:5.49|P-value:1.19E-7 ID:SGTC_1922|Compound:5578187|FD-Score:6.21|P-value:2.58E-9 ID:SGTC_1950|Compound:st077106|FD-Score:3.72|P-value:2.28E-4 ID:SGTC_2003|Compound:4023581|FD-Score:7.89|P-value:5.95E-14 ID:SGTC_2005|Compound:4024620|FD-Score:-4.98|P-value:1.45E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.50|P-value:4.97E-4 ID:SGTC_2032|Compound:5185112|FD-Score:4.10|P-value:5.74E-5 ID:SGTC_2038|Compound:4100038|FD-Score:5.24|P-value:4.02E-7 ID:SGTC_2082|Compound:5219693|FD-Score:5.41|P-value:1.80E-7 ID:SGTC_2349|Compound:9023730|FD-Score:-3.99|P-value:9.13E-5 ID:SGTC_2445|Compound:5742597|FD-Score:4.02|P-value:7.61E-5 ID:SGTC_2447|Compound:5741742|FD-Score:8.30|P-value:3.04E-15 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:6.24|P-value:2.19E-9 ID:SGTC_2472|Compound:5377652|FD-Score:5.05|P-value:9.99E-7 ID:SGTC_2486|Compound:5283202|FD-Score:5.84|P-value:1.92E-8 ID:SGTC_2488|Compound:5268135|FD-Score:5.09|P-value:8.37E-7 ID:SGTC_2515|Compound:chrysin|FD-Score:3.40|P-value:6.80E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.39|P-value:1.83E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.24|P-value:1.87E-18 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:12.50|P-value:3.06E-32 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.82|P-value:1.70E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.54|P-value:4.49E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:7.89|P-value:5.94E-14 ID:SGTC_2781|Compound:5567276|FD-Score:-4.84|P-value:2.79E-6 ID:SGTC_2784|Compound:7567673|FD-Score:4.99|P-value:1.32E-6 ID:SGTC_2788|Compound:5282734|FD-Score:5.48|P-value:1.25E-7 ID:SGTC_2790|Compound:5423970|FD-Score:6.87|P-value:5.08E-11 ID:SGTC_2814|Compound:7977482|FD-Score:3.29|P-value:9.68E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.05|P-value:6.81E-5 ID:SGTC_2971|Compound:9092186|FD-Score:4.84|P-value:2.63E-6 ID:SGTC_2981|Compound:9013952|FD-Score:10.20|P-value:6.73E-22 ID:SGTC_3041|Compound:9091929|FD-Score:3.33|P-value:8.47E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.68|P-value:2.76E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-4.68|P-value:5.62E-6 ID:SGTC_3237|Compound:9133362|FD-Score:3.75|P-value:2.11E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.68|P-value:2.66E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.76|P-value:3.71E-6 ID:SGTC_10|Compound:0279-0212|FD-Score:3.88|P-value:1.28E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.51|P-value:1.10E-7 ID:SGTC_38|Compound:0929-0051|FD-Score:3.29|P-value:9.83E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:4.05|P-value:7.01E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:12.00|P-value:1.28E-29 ID:SGTC_67|Compound:0335-0847|FD-Score:5.84|P-value:1.97E-8 ID:SGTC_86|Compound:0417-1630|FD-Score:7.80|P-value:1.09E-13 ID:SGTC_142|Compound:0335-0849|FD-Score:8.01|P-value:2.44E-14 ID:SGTC_153|Compound:4077-0274|FD-Score:3.61|P-value:3.39E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.98|P-value:9.14E-5 ID:SGTC_200|Compound:k038-0079|FD-Score:3.49|P-value:5.09E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:8.08|P-value:1.55E-14 ID:SGTC_206|Compound:4546-0025|FD-Score:5.80|P-value:2.48E-8 ID:SGTC_207|Compound:4185-0010|FD-Score:3.45|P-value:5.77E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:4.75|P-value:3.95E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:8.09|P-value:1.44E-14 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.47|P-value:1.38E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:9.60|P-value:8.58E-20 ID:SGTC_577|Compound:r071-0003|FD-Score:3.47|P-value:5.39E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:5.30|P-value:3.09E-7 ID:SGTC_786|Compound:4544-0069|FD-Score:7.07|P-value:1.49E-11 ID:SGTC_788|Compound:1182-0090|FD-Score:5.11|P-value:7.62E-7 ID:SGTC_819|Compound:1013-0258|FD-Score:8.00|P-value:2.60E-14 ID:SGTC_841|Compound:0251-0215|FD-Score:6.99|P-value:2.50E-11 ID:SGTC_892|Compound:0929-0076|FD-Score:3.84|P-value:1.53E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.61|P-value:3.36E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.44|P-value:6.08E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.59|P-value:3.81E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.32|P-value:9.29E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.33|P-value:8.65E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.80|P-value:1.76E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.97|P-value:9.58E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.80|P-value:1.75E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.86|P-value:1.42E-4 ID:SGTC_1488|Compound:4013-0813|FD-Score:-3.42|P-value:6.57E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.50|P-value:4.95E-10 ID:SGTC_1687|Compound:st020778|FD-Score:11.20|P-value:4.05E-26 ID:SGTC_1694|Compound:st024011|FD-Score:4.01|P-value:7.95E-5 ID:SGTC_1729|Compound:st035615|FD-Score:4.12|P-value:5.31E-5 ID:SGTC_1736|Compound:st036774|FD-Score:3.45|P-value:5.82E-4 ID:SGTC_1826|Compound:st055655|FD-Score:3.32|P-value:8.86E-4 ID:SGTC_1881|Compound:5652484|FD-Score:5.34|P-value:2.59E-7 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.89|P-value:1.25E-4 ID:SGTC_1921|Compound:5740136|FD-Score:5.49|P-value:1.19E-7 ID:SGTC_1922|Compound:5578187|FD-Score:6.21|P-value:2.58E-9 ID:SGTC_1950|Compound:st077106|FD-Score:3.72|P-value:2.28E-4 ID:SGTC_2003|Compound:4023581|FD-Score:7.89|P-value:5.95E-14 ID:SGTC_2005|Compound:4024620|FD-Score:-4.98|P-value:1.45E-6 ID:SGTC_2006|Compound:4011487|FD-Score:3.50|P-value:4.97E-4 ID:SGTC_2032|Compound:5185112|FD-Score:4.10|P-value:5.74E-5 ID:SGTC_2038|Compound:4100038|FD-Score:5.24|P-value:4.02E-7 ID:SGTC_2082|Compound:5219693|FD-Score:5.41|P-value:1.80E-7 ID:SGTC_2349|Compound:9023730|FD-Score:-3.99|P-value:9.13E-5 ID:SGTC_2445|Compound:5742597|FD-Score:4.02|P-value:7.61E-5 ID:SGTC_2447|Compound:5741742|FD-Score:8.30|P-value:3.04E-15 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:6.24|P-value:2.19E-9 ID:SGTC_2472|Compound:5377652|FD-Score:5.05|P-value:9.99E-7 ID:SGTC_2486|Compound:5283202|FD-Score:5.84|P-value:1.92E-8 ID:SGTC_2488|Compound:5268135|FD-Score:5.09|P-value:8.37E-7 ID:SGTC_2515|Compound:chrysin|FD-Score:3.40|P-value:6.80E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.39|P-value:1.83E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.24|P-value:1.87E-18 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:12.50|P-value:3.06E-32 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.82|P-value:1.70E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.54|P-value:4.49E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:7.89|P-value:5.94E-14 ID:SGTC_2781|Compound:5567276|FD-Score:-4.84|P-value:2.79E-6 ID:SGTC_2784|Compound:7567673|FD-Score:4.99|P-value:1.32E-6 ID:SGTC_2788|Compound:5282734|FD-Score:5.48|P-value:1.25E-7 ID:SGTC_2790|Compound:5423970|FD-Score:6.87|P-value:5.08E-11 ID:SGTC_2814|Compound:7977482|FD-Score:3.29|P-value:9.68E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.05|P-value:6.81E-5 ID:SGTC_2971|Compound:9092186|FD-Score:4.84|P-value:2.63E-6 ID:SGTC_2981|Compound:9013952|FD-Score:10.20|P-value:6.73E-22 ID:SGTC_3041|Compound:9091929|FD-Score:3.33|P-value:8.47E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.68|P-value:2.76E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-4.68|P-value:5.62E-6 ID:SGTC_3237|Compound:9133362|FD-Score:3.75|P-value:2.11E-4 ID:SGTC_3291|Compound:9121177|FD-Score:3.68|P-value:2.66E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.76|P-value:3.71E-6

Top fitness defect scores for YKL077W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2588 indole-3-carbinol 12.50 100.0 µM 3.06E-32 1 3.29E-36
2 SGTC_50 0929-0078 12.00 56.4 µM 1.28E-29 4 3.04E-33
3 SGTC_1687 st020778 11.20 55.4 µM 4.05E-26 43 2.75E-29
4 SGTC_2981 9013952 10.20 39.0 µM 6.73E-22 2 1.62E-24
5 SGTC_528 3492-0019 9.60 172.0 µM 8.58E-20 calcium & mitochondrial duress 42 3.88E-22
6 SGTC_2582 agaric acid 9.24 100.0 µM 1.87E-18 redox potentiating 80 1.26E-20
7 SGTC_2447 5741742 8.30 33.8 µM 3.04E-15 1 5.36E-17
8 SGTC_498 2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 8.09 150.0 µM 1.44E-14 Golgi 7 3.10E-16
9 SGTC_204 1543-0184 8.08 62.4 µM 1.55E-14 TRP & mitochondrial translation 5 3.37E-16
10 SGTC_142 0335-0849 8.01 62.7 µM 2.44E-14 ubiquinone biosynthesis & proteasome 44 5.62E-16
11 SGTC_819 1013-0258 8.00 27.7 µM 2.60E-14 13 6.04E-16
12 SGTC_2752 diclazuril 7.89 33.1 µM 5.94E-14 38 1.54E-15
13 SGTC_2003 4023581 7.89 200.0 µM 5.95E-14 cell wall signaling 8 1.54E-15
14 SGTC_86 0417-1630 7.80 471.9 µM 1.09E-13 ubiquinone biosynthesis & proteasome 60 3.06E-15
15 SGTC_786 4544-0069 7.07 423.0 µM 1.49E-11 5 7.87E-13
16 SGTC_841 0251-0215 6.99 91.2 µM 2.50E-11 29 1.41E-12
17 SGTC_2790 5423970 6.87 71.4 µM 5.08E-11 endomembrane recycling 19 3.14E-12
18 SGTC_1495 4469-0029 6.50 237.0 µM 4.95E-10 43 4.10E-11
19 SGTC_2454 5-benzylidenerhodanine 6.24 10.4 µM 2.19E-9 TRP & mitochondrial translation 10 2.19E-10
20 SGTC_1922 5578187 6.21 25.0 µM 2.58E-9 TRP & mitochondrial translation 4 2.64E-10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.330 5.82E-86 YGL007W_d BRP1_d hom Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
0.308 1.58E-74 YGL008C PMA1 het Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.276 9.98E-60 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.220 8.50E-38 YMR123W PKR1 hom V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
0.214 5.62E-36 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.207 7.33E-34 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.206 1.26E-33 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.205 3.87E-33 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.202 3.81E-32 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.189 2.29E-28 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.189 3.14E-28 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.188 4.45E-28 YKR029C SET3 hom Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication
0.188 6.02E-28 YDR202C RAV2 hom Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
0.184 5.40E-27 YML122C_d YML122C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.183 1.03E-26 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2