YKL109W / HAP4

Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL109W / HAP4

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-3.93|P-value:5.61E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:-4.13|P-value:2.41E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:4.57|P-value:3.16E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:4.48|P-value:4.80E-6 ID:SGTC_128|Compound:0906-2981|FD-Score:3.51|P-value:2.56E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.72|P-value:1.54E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.32|P-value:5.63E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.21|P-value:1.58E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:-4.27|P-value:1.34E-5 ID:SGTC_175|Compound:3793-3120|FD-Score:-4.62|P-value:2.72E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:-5.64|P-value:1.42E-8 ID:SGTC_178|Compound:4092-0806|FD-Score:-12.30|P-value:5.64E-34 ID:SGTC_182|Compound:3448-4371|FD-Score:4.22|P-value:1.51E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:33.30|P-value:2.75E-236 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.42|P-value:3.64E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.27|P-value:6.00E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:4.07|P-value:2.86E-5 ID:SGTC_322|Compound:k048-0136|FD-Score:3.43|P-value:3.40E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.59|P-value:2.06E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.26|P-value:6.38E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.67|P-value:1.95E-6 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.63|P-value:1.79E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.76|P-value:1.11E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.75|P-value:1.14E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.19|P-value:1.74E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:5.67|P-value:1.06E-8 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.28|P-value:6.38E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.90|P-value:6.36E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:-3.65|P-value:1.67E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.32|P-value:9.82E-6 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.18|P-value:8.28E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.64|P-value:1.60E-4 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.98|P-value:4.40E-7 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.67|P-value:1.44E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.20|P-value:1.66E-5 ID:SGTC_1583|Compound:clindamycin|FD-Score:4.49|P-value:4.57E-6 ID:SGTC_1635|Compound:st007035|FD-Score:6.58|P-value:4.07E-11 ID:SGTC_1727|Compound:st034309|FD-Score:-3.86|P-value:7.58E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.28|P-value:6.47E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.23|P-value:7.50E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.24|P-value:7.34E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-3.93|P-value:5.71E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.46|P-value:3.08E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.60|P-value:1.85E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.46|P-value:3.13E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.18|P-value:8.29E-4 ID:SGTC_2157|Compound:5649910|FD-Score:3.15|P-value:9.20E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.44|P-value:3.37E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.92|P-value:5.67E-7 ID:SGTC_2336|Compound:9001819|FD-Score:4.44|P-value:5.65E-6 ID:SGTC_2365|Compound:9042475|FD-Score:4.24|P-value:1.38E-5 ID:SGTC_2411|Compound:st077133|FD-Score:4.14|P-value:2.14E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.37|P-value:5.76E-8 ID:SGTC_2436|Compound:5633522|FD-Score:3.44|P-value:3.29E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.25|P-value:6.50E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:1.95E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.18|P-value:8.27E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.26|P-value:6.75E-4 ID:SGTC_2859|Compound:9028643|FD-Score:4.21|P-value:1.61E-5 ID:SGTC_2881|Compound:9058953|FD-Score:-3.75|P-value:1.15E-4 ID:SGTC_2886|Compound:9058944|FD-Score:3.33|P-value:4.86E-4 ID:SGTC_2895|Compound:9054447|FD-Score:-3.19|P-value:8.75E-4 ID:SGTC_2899|Compound:9053889|FD-Score:4.31|P-value:1.03E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.69|P-value:1.32E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.65|P-value:1.71E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.66|P-value:2.26E-6 ID:SGTC_3318|Compound:9139014|FD-Score:3.14|P-value:9.31E-4 ID:SGTC_3345|Compound:9148410|FD-Score:4.11|P-value:2.39E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.93|P-value:5.61E-5 ID:SGTC_71|Compound:0417-1750|FD-Score:-4.13|P-value:2.41E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:4.57|P-value:3.16E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:4.48|P-value:4.80E-6 ID:SGTC_128|Compound:0906-2981|FD-Score:3.51|P-value:2.56E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.72|P-value:1.54E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.32|P-value:5.63E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.21|P-value:1.58E-5 ID:SGTC_156|Compound:3474-0145|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:-4.27|P-value:1.34E-5 ID:SGTC_175|Compound:3793-3120|FD-Score:-4.62|P-value:2.72E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:-5.64|P-value:1.42E-8 ID:SGTC_178|Compound:4092-0806|FD-Score:-12.30|P-value:5.64E-34 ID:SGTC_182|Compound:3448-4371|FD-Score:4.22|P-value:1.51E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:33.30|P-value:2.75E-236 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.42|P-value:3.64E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.27|P-value:6.00E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:4.07|P-value:2.86E-5 ID:SGTC_322|Compound:k048-0136|FD-Score:3.43|P-value:3.40E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.59|P-value:2.06E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.26|P-value:6.38E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:4.67|P-value:1.95E-6 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.63|P-value:1.79E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.76|P-value:1.11E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.75|P-value:1.14E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.19|P-value:1.74E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:5.67|P-value:1.06E-8 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.28|P-value:6.38E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-3.90|P-value:6.36E-5 ID:SGTC_1014|Compound:4130-3005|FD-Score:-3.65|P-value:1.67E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.32|P-value:9.82E-6 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.18|P-value:8.28E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:3.64|P-value:1.60E-4 ID:SGTC_1422|Compound:4031-0024|FD-Score:4.98|P-value:4.40E-7 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.67|P-value:1.44E-4 ID:SGTC_1567|Compound:st069303|FD-Score:4.20|P-value:1.66E-5 ID:SGTC_1583|Compound:clindamycin|FD-Score:4.49|P-value:4.57E-6 ID:SGTC_1635|Compound:st007035|FD-Score:6.58|P-value:4.07E-11 ID:SGTC_1727|Compound:st034309|FD-Score:-3.86|P-value:7.58E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.28|P-value:6.47E-4 ID:SGTC_1735|Compound:st034310|FD-Score:-3.23|P-value:7.50E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.24|P-value:7.34E-4 ID:SGTC_1755|Compound:st041960|FD-Score:-3.93|P-value:5.71E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.46|P-value:3.08E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.60|P-value:1.85E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.46|P-value:3.13E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.18|P-value:8.29E-4 ID:SGTC_2157|Compound:5649910|FD-Score:3.15|P-value:9.20E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.44|P-value:3.37E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.92|P-value:5.67E-7 ID:SGTC_2336|Compound:9001819|FD-Score:4.44|P-value:5.65E-6 ID:SGTC_2365|Compound:9042475|FD-Score:4.24|P-value:1.38E-5 ID:SGTC_2411|Compound:st077133|FD-Score:4.14|P-value:2.14E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.37|P-value:5.76E-8 ID:SGTC_2436|Compound:5633522|FD-Score:3.44|P-value:3.29E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.25|P-value:6.50E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:1.95E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.18|P-value:8.27E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.26|P-value:6.75E-4 ID:SGTC_2859|Compound:9028643|FD-Score:4.21|P-value:1.61E-5 ID:SGTC_2881|Compound:9058953|FD-Score:-3.75|P-value:1.15E-4 ID:SGTC_2886|Compound:9058944|FD-Score:3.33|P-value:4.86E-4 ID:SGTC_2895|Compound:9054447|FD-Score:-3.19|P-value:8.75E-4 ID:SGTC_2899|Compound:9053889|FD-Score:4.31|P-value:1.03E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.69|P-value:1.32E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.65|P-value:1.71E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.66|P-value:2.26E-6 ID:SGTC_3318|Compound:9139014|FD-Score:3.14|P-value:9.31E-4 ID:SGTC_3345|Compound:9148410|FD-Score:4.11|P-value:2.39E-5

Top fitness defect scores for YKL109W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 33.30 5.3 µM 2.75E-236 6 6.71E-243
2 SGTC_1635 st007035 6.58 42.4 µM 4.07E-11 124 2.36E-11
3 SGTC_635 k038-0028 5.67 24.7 µM 1.06E-8 ubiquinone biosynthesis & proteasome 18 7.09E-9
4 SGTC_2419 paclitaxel 5.37 166.5 µM 5.76E-8 9 4.04E-8
5 SGTC_1422 4031-0024 4.98 15.5 µM 4.40E-7 iron homeostasis 7 3.25E-7
6 SGTC_2325 6158266 4.92 200.0 µM 5.67E-7 11 4.22E-7
7 SGTC_134 0269-0018 4.72 6.9 µM 1.54E-6 107 1.18E-6
8 SGTC_402 epigallocatechin gallate 4.67 10.0 µM 1.95E-6 9 1.50E-6
9 SGTC_73 0327-0325 4.57 16.2 µM 3.16E-6 DNA damage response 31 2.46E-6
10 SGTC_1583 clindamycin 4.49 39.6 µM 4.57E-6 mitochondrial processes 6 3.58E-6
11 SGTC_116 0155-0182 4.48 31.8 µM 4.80E-6 mitochondrial response to ROS 57 3.77E-6
12 SGTC_2336 9001819 4.44 198.7 µM 5.65E-6 heme biosynthesis & mitochondrial translocase 35 4.45E-6
13 SGTC_1133 3473-0247 4.32 50.0 µM 9.82E-6 RSC complex & mRNA processing 38 7.86E-6
14 SGTC_2899 9053889 4.31 58.4 µM 1.03E-5 6 8.26E-6
15 SGTC_2365 9042475 4.24 200.0 µM 1.38E-5 25 1.11E-5
16 SGTC_182 3448-4371 4.22 550.5 µM 1.51E-5 20 1.22E-5
17 SGTC_139 0099-0334 4.21 718.6 µM 1.58E-5 20 1.28E-5
18 SGTC_2859 9028643 4.21 45.5 µM 1.61E-5 12 1.30E-5
19 SGTC_1567 st069303 4.20 68.4 µM 1.66E-5 mitochondrial processes 15 1.35E-5
20 SGTC_632 0781-2353 4.19 4.7 µM 1.74E-5 Golgi 34 1.41E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.575 4.01E-295 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.567 8.36E-284 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.566 4.25E-283 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.559 5.03E-275 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.556 2.52E-271 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.538 2.48E-251 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.522 3.80E-234 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.513 3.59E-225 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.501 4.34E-213 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.501 8.12E-213 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.494 5.63E-206 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.492 2.45E-204 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.491 5.79E-203 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.482 5.92E-195 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.478 6.00E-191 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p