YKL110C / KTI12

Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL110C / KTI12

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:4.54|P-value:7.10E-6 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.47|P-value:7.36E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:3.86|P-value:1.14E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.29|P-value:8.53E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.83|P-value:9.96E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:-4.42|P-value:8.86E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:4.45|P-value:1.07E-5 ID:SGTC_588|Compound:3970-1144|FD-Score:-5.62|P-value:2.59E-8 ID:SGTC_599|Compound:1935-0052|FD-Score:3.37|P-value:6.59E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.39|P-value:6.21E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.76|P-value:1.75E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.93|P-value:8.91E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.62|P-value:2.72E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.31|P-value:6.40E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.46|P-value:8.86E-8 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.61|P-value:2.87E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.84|P-value:1.22E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.39|P-value:6.10E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.88|P-value:1.05E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.65|P-value:1.93E-4 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.52|P-value:3.95E-4 ID:SGTC_1625|Compound:st003711|FD-Score:4.07|P-value:5.10E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.51|P-value:3.21E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.52|P-value:3.12E-4 ID:SGTC_1768|Compound:st044301|FD-Score:5.05|P-value:6.65E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:15.40|P-value:4.24E-50 ID:SGTC_1905|Compound:st060207|FD-Score:3.93|P-value:8.64E-5 ID:SGTC_1915|Compound:st067105|FD-Score:4.26|P-value:2.31E-5 ID:SGTC_1925|Compound:st057770|FD-Score:3.26|P-value:9.58E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.71|P-value:3.30E-6 ID:SGTC_2059|Compound:5238311|FD-Score:3.35|P-value:7.06E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.28|P-value:7.09E-4 ID:SGTC_2118|Compound:5526350|FD-Score:3.31|P-value:7.99E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.22|P-value:8.59E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.34|P-value:7.34E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.72|P-value:1.94E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.95|P-value:8.25E-5 ID:SGTC_2556|Compound:citropten|FD-Score:6.20|P-value:1.48E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.31|P-value:1.92E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.60|P-value:1.77E-24 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.09|P-value:4.63E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.32|P-value:7.73E-4 ID:SGTC_2959|Compound:9079155|FD-Score:4.97|P-value:9.96E-7 ID:SGTC_2989|Compound:9073649|FD-Score:3.48|P-value:4.54E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.27|P-value:9.16E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.67|P-value:2.32E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.48|P-value:4.55E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.54|P-value:7.10E-6 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.47|P-value:7.36E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:3.86|P-value:1.14E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.29|P-value:8.53E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.83|P-value:9.96E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:-4.42|P-value:8.86E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:4.45|P-value:1.07E-5 ID:SGTC_588|Compound:3970-1144|FD-Score:-5.62|P-value:2.59E-8 ID:SGTC_599|Compound:1935-0052|FD-Score:3.37|P-value:6.59E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.39|P-value:6.21E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.76|P-value:1.75E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:3.93|P-value:8.91E-5 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.62|P-value:2.72E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.31|P-value:6.40E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:5.46|P-value:8.86E-8 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.61|P-value:2.87E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.84|P-value:1.22E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.39|P-value:6.10E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.88|P-value:1.05E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.65|P-value:1.93E-4 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.52|P-value:3.95E-4 ID:SGTC_1625|Compound:st003711|FD-Score:4.07|P-value:5.10E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.51|P-value:3.21E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.52|P-value:3.12E-4 ID:SGTC_1768|Compound:st044301|FD-Score:5.05|P-value:6.65E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:15.40|P-value:4.24E-50 ID:SGTC_1905|Compound:st060207|FD-Score:3.93|P-value:8.64E-5 ID:SGTC_1915|Compound:st067105|FD-Score:4.26|P-value:2.31E-5 ID:SGTC_1925|Compound:st057770|FD-Score:3.26|P-value:9.58E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.71|P-value:3.30E-6 ID:SGTC_2059|Compound:5238311|FD-Score:3.35|P-value:7.06E-4 ID:SGTC_2102|Compound:5377816|FD-Score:-3.28|P-value:7.09E-4 ID:SGTC_2118|Compound:5526350|FD-Score:3.31|P-value:7.99E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:-3.22|P-value:8.59E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.34|P-value:7.34E-4 ID:SGTC_2135|Compound:5326629|FD-Score:3.72|P-value:1.94E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.95|P-value:8.25E-5 ID:SGTC_2556|Compound:citropten|FD-Score:6.20|P-value:1.48E-9 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.31|P-value:1.92E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.60|P-value:1.77E-24 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.09|P-value:4.63E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.32|P-value:7.73E-4 ID:SGTC_2959|Compound:9079155|FD-Score:4.97|P-value:9.96E-7 ID:SGTC_2989|Compound:9073649|FD-Score:3.48|P-value:4.54E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.27|P-value:9.16E-4 ID:SGTC_3089|Compound:9117425|FD-Score:3.67|P-value:2.32E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.48|P-value:4.55E-4

Top fitness defect scores for YKL110C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 15.40 4.0 nM 4.24E-50 13 4.29E-54
2 SGTC_2679 rapamycin 10.60 1.0 nM 1.77E-24 22 2.11E-26
3 SGTC_2556 citropten 6.20 84.6 µM 1.48E-9 2 2.91E-10
4 SGTC_800 3448-7362 5.76 155.0 µM 1.75E-8 translation 57 4.21E-9
5 SGTC_1107 amlodipine 5.46 47.0 µM 8.86E-8 7 2.43E-8
6 SGTC_1768 st044301 5.05 37.2 µM 6.65E-7 5 2.16E-7
7 SGTC_2959 9079155 4.97 11.0 µM 9.96E-7 12 3.34E-7
8 SGTC_2026 5148700 4.71 152.0 µM 3.30E-6 8 1.22E-6
9 SGTC_118 0083-0097 4.54 135.3 µM 7.10E-6 translation 84 2.80E-6
10 SGTC_582 0987-0078 4.45 156.0 µM 1.07E-5 calcium & mitochondrial duress 50 4.34E-6
11 SGTC_2582 agaric acid 4.31 100.0 µM 1.92E-5 redox potentiating 278 8.21E-6
12 SGTC_1915 st067105 4.26 30.6 µM 2.31E-5 RNA pol III & RNase P/MRP 35 1.00E-5
13 SGTC_2688 kanamycin b 4.09 96.7 µM 4.63E-5 calcium & mitochondrial duress 349 2.13E-5
14 SGTC_1625 st003711 4.07 30.1 µM 5.10E-5 calcium & mitochondrial duress 32 2.36E-5
15 SGTC_2283 7944793 3.95 200.0 µM 8.25E-5 42 3.98E-5
16 SGTC_1905 st060207 3.93 84.5 µM 8.64E-5 12 4.19E-5
17 SGTC_879 0831-1030 3.93 162.0 µM 8.91E-5 46 4.33E-5
18 SGTC_1392 3455-1037 3.88 33.9 µM 1.05E-4 18 5.17E-5
19 SGTC_184 1494-0393 3.86 24.7 µM 1.14E-4 63 5.64E-5
20 SGTC_1187 1391-0724 3.84 117.0 µM 1.22E-4 amide catabolism 18 6.09E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.412 1.87E-137 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.405 1.02E-132 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.380 9.31E-116 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.353 5.79E-99 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.351 4.07E-98 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.323 1.76E-82 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.320 1.35E-80 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.310 1.88E-75 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.299 2.73E-70 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.280 2.19E-61 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.275 4.69E-59 YDR158W HOM2 hom Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.274 9.51E-59 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.274 1.01E-58 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.273 2.12E-58 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.272 6.88E-58 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity