YKL122C / SRP21

Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL122C / SRP21

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.91|P-value:6.75E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.99|P-value:5.58E-6 ID:SGTC_328|Compound:k915-0120|FD-Score:2.86|P-value:8.06E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.86|P-value:1.08E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.47|P-value:6.84E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.63|P-value:3.30E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.21|P-value:2.06E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.42|P-value:8.36E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:3.13|P-value:2.85E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.03|P-value:5.27E-15 ID:SGTC_1179|Compound:1021-1431|FD-Score:2.82|P-value:9.59E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.54|P-value:4.94E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.12|P-value:3.00E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-5.20|P-value:5.43E-9 ID:SGTC_1729|Compound:st035615|FD-Score:-3.06|P-value:3.81E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-2.97|P-value:5.46E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-4.19|P-value:2.01E-6 ID:SGTC_1866|Compound:st057638|FD-Score:-2.89|P-value:7.40E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.19|P-value:2.31E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.09|P-value:3.31E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-2.86|P-value:8.19E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.07|P-value:3.76E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-7.26|P-value:6.96E-16 ID:SGTC_2102|Compound:5377816|FD-Score:3.43|P-value:7.86E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-3.15|P-value:2.72E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.80|P-value:1.43E-5 ID:SGTC_2184|Compound:6075246|FD-Score:3.53|P-value:4.96E-5 ID:SGTC_2347|Compound:9049783|FD-Score:2.89|P-value:7.32E-4 ID:SGTC_2391|Compound:6285546|FD-Score:-2.83|P-value:9.36E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-4.60|P-value:2.08E-7 ID:SGTC_2457|Compound:5731572|FD-Score:2.83|P-value:9.14E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.45|P-value:7.16E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:3.93|P-value:7.34E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.21|P-value:1.82E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-4.42|P-value:5.91E-7 ID:SGTC_2835|Compound:9006588|FD-Score:-3.85|P-value:1.17E-5 ID:SGTC_2952|Compound:9073731|FD-Score:3.21|P-value:1.99E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.41|P-value:8.69E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.37|P-value:1.04E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.26|P-value:1.32E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.91|P-value:6.75E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.99|P-value:5.58E-6 ID:SGTC_328|Compound:k915-0120|FD-Score:2.86|P-value:8.06E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.86|P-value:1.08E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.47|P-value:6.84E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.63|P-value:3.30E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.21|P-value:2.06E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.42|P-value:8.36E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:3.13|P-value:2.85E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.03|P-value:5.27E-15 ID:SGTC_1179|Compound:1021-1431|FD-Score:2.82|P-value:9.59E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.54|P-value:4.94E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.12|P-value:3.00E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-5.20|P-value:5.43E-9 ID:SGTC_1729|Compound:st035615|FD-Score:-3.06|P-value:3.81E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-2.97|P-value:5.46E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-4.19|P-value:2.01E-6 ID:SGTC_1866|Compound:st057638|FD-Score:-2.89|P-value:7.40E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.19|P-value:2.31E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.09|P-value:3.31E-4 ID:SGTC_2003|Compound:4023581|FD-Score:-2.86|P-value:8.19E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.07|P-value:3.76E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-7.26|P-value:6.96E-16 ID:SGTC_2102|Compound:5377816|FD-Score:3.43|P-value:7.86E-5 ID:SGTC_2128|Compound:5255449|FD-Score:-3.15|P-value:2.72E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.80|P-value:1.43E-5 ID:SGTC_2184|Compound:6075246|FD-Score:3.53|P-value:4.96E-5 ID:SGTC_2347|Compound:9049783|FD-Score:2.89|P-value:7.32E-4 ID:SGTC_2391|Compound:6285546|FD-Score:-2.83|P-value:9.36E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-4.60|P-value:2.08E-7 ID:SGTC_2457|Compound:5731572|FD-Score:2.83|P-value:9.14E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.45|P-value:7.16E-5 ID:SGTC_2516|Compound:gossypol|FD-Score:3.93|P-value:7.34E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.21|P-value:1.82E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-4.42|P-value:5.91E-7 ID:SGTC_2835|Compound:9006588|FD-Score:-3.85|P-value:1.17E-5 ID:SGTC_2952|Compound:9073731|FD-Score:3.21|P-value:1.99E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.41|P-value:8.69E-5 ID:SGTC_3103|Compound:9120822|FD-Score:3.37|P-value:1.04E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.26|P-value:1.32E-6

Top fitness defect scores for YKL122C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3137 9097352 4.26 49.5 µM 1.32E-6 4 1.00E-5 0.11
2 SGTC_69 0096-0274 3.99 206.7 µM 5.58E-6 mitochondrial processes 36 3.32E-5 0.04
3 SGTC_2516 gossypol 3.93 71.5 µM 7.34E-6 9 4.18E-5 0.27
4 SGTC_2184 6075246 3.53 200.0 µM 4.96E-5 3 2.05E-4 0.39
5 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.45 3.7 µM 7.16E-5 18 2.79E-4 0.14
6 SGTC_2102 5377816 3.43 72.8 µM 7.86E-5 7 3.01E-4 0.01
7 SGTC_845 0330-0138 3.42 4.5 µM 8.36E-5 10 3.17E-4 0.04
8 SGTC_3103 9120822 3.37 49.5 µM 1.04E-4 15 3.82E-4 0.04
9 SGTC_2952 9073731 3.21 13.8 µM 1.99E-4 tubulin folding & SWR complex 17 6.54E-4 0.08
10 SGTC_843 0310-0051 3.21 143.0 µM 2.06E-4 6 6.72E-4 0.01
11 SGTC_967 1306-0166 3.13 352.0 µM 2.85E-4 5 8.82E-4 0.04
12 SGTC_1973 st070275 3.09 50.2 µM 3.31E-4 5 0.00100 0.02
13 SGTC_28 pentachloropyridine N-oxide 2.91 1.6 µM 6.75E-4 TSC3-RPN4 8 0.00181 0.00
14 SGTC_2347 9049783 2.89 174.8 µM 7.32E-4 11 0.00194 7.00E-4
15 SGTC_328 k915-0120 2.86 63.5 µM 8.06E-4 TSC3-RPN4 7 0.00210 0.09
16 SGTC_2457 5731572 2.83 11.7 µM 9.14E-4 12 0.00234 0.02
17 SGTC_1179 1021-1431 2.82 9.3 µM 9.59E-4 19 0.00244 0.02
18 SGTC_781 4335-2806 2.79 94.4 µM 0.00106 15 0.00264 0.04
19 SGTC_2751 anetholtrithion 2.78 17.2 µM 0.00110 20 0.00273 0.20
20 SGTC_2187 6125751 2.74 200.0 µM 0.00126 RSC complex & mRNA processing 29 0.00307 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.227 1.26E-40 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.221 2.43E-38 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.216 1.07E-36 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.215 1.87E-36 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.207 6.37E-34 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.204 8.47E-33 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.202 2.56E-32 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.198 4.64E-31 YDR037W KRS1 het Lysyl-tRNA synthetase
0.197 1.25E-30 YBR090C_p YBR090C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.193 2.19E-29 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.192 2.40E-29 YMR150C IMP1 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.191 8.16E-29 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.189 2.96E-28 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.185 3.08E-27 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.184 7.98E-27 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function