YKL140W / TGL1

Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL140W / TGL1

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:4.13|P-value:4.46E-7 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.25|P-value:5.36E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:2.65|P-value:7.94E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:2.93|P-value:2.45E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.96|P-value:2.68E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-2.84|P-value:4.49E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.57|P-value:2.83E-11 ID:SGTC_939|Compound:1165-0479|FD-Score:3.32|P-value:3.87E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:2.99|P-value:1.80E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.23|P-value:7.79E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.31|P-value:5.18E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.45|P-value:2.57E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-2.70|P-value:7.92E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.30|P-value:1.57E-7 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.05|P-value:1.43E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:2.89|P-value:2.86E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.23|P-value:3.33E-7 ID:SGTC_1627|Compound:st003713|FD-Score:-3.84|P-value:3.27E-6 ID:SGTC_1729|Compound:st035615|FD-Score:7.03|P-value:3.78E-17 ID:SGTC_1732|Compound:st034308|FD-Score:-3.24|P-value:7.44E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-2.76|P-value:6.13E-4 ID:SGTC_1825|Compound:st052143|FD-Score:2.81|P-value:4.01E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.01|P-value:1.70E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.31|P-value:4.13E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.21|P-value:6.56E-5 ID:SGTC_1896|Compound:st058441|FD-Score:2.72|P-value:5.96E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.78|P-value:5.62E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.72|P-value:5.84E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.70|P-value:5.31E-6 ID:SGTC_2363|Compound:9053361|FD-Score:3.61|P-value:8.60E-6 ID:SGTC_2397|Compound:5161689|FD-Score:2.92|P-value:2.48E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.69|P-value:7.33E-6 ID:SGTC_2412|Compound:st077135|FD-Score:2.86|P-value:3.27E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.59|P-value:9.80E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.78|P-value:5.65E-4 ID:SGTC_2870|Compound:9038528|FD-Score:2.92|P-value:2.53E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.68|P-value:6.84E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-2.91|P-value:3.27E-4 ID:SGTC_3336|Compound:9143619|FD-Score:2.65|P-value:7.97E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.06|P-value:1.35E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.13|P-value:4.46E-7 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.25|P-value:5.36E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:2.65|P-value:7.94E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:2.93|P-value:2.45E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.96|P-value:2.68E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-2.84|P-value:4.49E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.57|P-value:2.83E-11 ID:SGTC_939|Compound:1165-0479|FD-Score:3.32|P-value:3.87E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:2.99|P-value:1.80E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.23|P-value:7.79E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.31|P-value:5.18E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.45|P-value:2.57E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-2.70|P-value:7.92E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.30|P-value:1.57E-7 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.05|P-value:1.43E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:2.89|P-value:2.86E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.23|P-value:3.33E-7 ID:SGTC_1627|Compound:st003713|FD-Score:-3.84|P-value:3.27E-6 ID:SGTC_1729|Compound:st035615|FD-Score:7.03|P-value:3.78E-17 ID:SGTC_1732|Compound:st034308|FD-Score:-3.24|P-value:7.44E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-2.76|P-value:6.13E-4 ID:SGTC_1825|Compound:st052143|FD-Score:2.81|P-value:4.01E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.01|P-value:1.70E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.31|P-value:4.13E-5 ID:SGTC_1895|Compound:st058434|FD-Score:3.21|P-value:6.56E-5 ID:SGTC_1896|Compound:st058441|FD-Score:2.72|P-value:5.96E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.78|P-value:5.62E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.72|P-value:5.84E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.70|P-value:5.31E-6 ID:SGTC_2363|Compound:9053361|FD-Score:3.61|P-value:8.60E-6 ID:SGTC_2397|Compound:5161689|FD-Score:2.92|P-value:2.48E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.69|P-value:7.33E-6 ID:SGTC_2412|Compound:st077135|FD-Score:2.86|P-value:3.27E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.59|P-value:9.80E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.78|P-value:5.65E-4 ID:SGTC_2870|Compound:9038528|FD-Score:2.92|P-value:2.53E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.68|P-value:6.84E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-2.91|P-value:3.27E-4 ID:SGTC_3336|Compound:9143619|FD-Score:2.65|P-value:7.97E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.06|P-value:1.35E-4

Top fitness defect scores for YKL140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1729 st035615 7.03 46.0 µM 3.78E-17 6 1.02E-12
2 SGTC_1134 3477-0009 4.30 42.5 µM 1.57E-7 38 8.38E-6
3 SGTC_41 0478-0632 4.13 82.6 µM 4.46E-7 26 1.78E-5
4 SGTC_2123 5259487 3.70 200.0 µM 5.31E-6 23 1.07E-4
5 SGTC_2363 9053361 3.61 176.0 µM 8.60E-6 Golgi 44 1.52E-4
6 SGTC_939 1165-0479 3.32 45.3 µM 3.87E-5 62 4.50E-4
7 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.31 14.9 µM 4.13E-5 31 4.73E-4
8 SGTC_112 phenothiazine 3.25 287.2 µM 5.36E-5 unfolded protein response 28 5.71E-4
9 SGTC_1895 st058434 3.21 18.3 µM 6.56E-5 37 6.61E-4
10 SGTC_3342 9147791 3.06 14.5 µM 1.35E-4 48 0.00112
11 SGTC_1208 2323-2347 3.05 50.3 µM 1.43E-4 38 0.00116
12 SGTC_1889 st059080 3.01 5.2 µM 1.70E-4 57 0.00132
13 SGTC_940 3232-1287 2.99 54.8 µM 1.80E-4 NEO1 84 0.00137
14 SGTC_381 3461-2219 2.93 42.7 µM 2.45E-4 copper-dependent oxidative stress 65 0.00172
15 SGTC_2397 5161689 2.92 200.0 µM 2.48E-4 90 0.00174
16 SGTC_2870 9038528 2.92 13.0 µM 2.53E-4 67 0.00176
17 SGTC_1211 1056-0033 2.89 32.3 µM 2.86E-4 42 0.00193
18 SGTC_2412 st077135 2.86 33.4 µM 3.27E-4 88 0.00212
19 SGTC_1825 st052143 2.81 62.6 µM 4.01E-4 56 0.00247
20 SGTC_2014 4013930 2.72 200.0 µM 5.84E-4 119 0.00324

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.273 3.34E-58 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.269 7.99E-57 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.255 4.56E-51 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.250 8.47E-49 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.231 8.13E-42 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.226 6.15E-40 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.215 2.28E-36 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.213 1.31E-35 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.212 1.95E-35 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.209 2.08E-34 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.204 5.15E-33 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.194 1.02E-29 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.193 1.77E-29 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.192 2.47E-29 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.183 1.03E-26 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER