HIPHOP chemogenomics database

YKL149C / DBR1

RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL149C / DBR1

Click on Significant Values for Screen Details

Top fitness defect scores for YKL149C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	6.63	3.7 µM	2.55E-11		8	1.68E-11
2	SGTC_522	1273-0010	5.41	11.6 µM	4.01E-8	60S ribosome export	7	3.21E-8
3	SGTC_3184	9108758	5.04	49.5 µM	2.75E-7		1	2.31E-7
4	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	5.00	4.6 µM	3.44E-7	heat shock/prefoldin	22	2.90E-7
5	SGTC_71	0417-1750	4.89	47.8 µM	5.83E-7	Golgi	27	4.98E-7
6	SGTC_6	0844-0013	4.83	19.8 µM	7.75E-7		43	6.68E-7
7	SGTC_2035	5154463	4.74	94.6 µM	1.21E-6		6	1.06E-6
8	SGTC_2001	4023502	4.48	57.7 µM	4.09E-6	mitochondrial processes	8	3.66E-6
9	SGTC_103	0844-0104	4.46	51.8 µM	4.62E-6	ERAD & cell cycle	122	4.15E-6
10	SGTC_978	1416-0512	4.37	87.4 µM	6.79E-6	redox potentiating	24	6.15E-6
11	SGTC_2800	7887935	4.30	81.8 µM	9.37E-6	RPP1 & pyrimidine depletion	22	8.55E-6
12	SGTC_2045	5106652	4.14	78.0 µM	1.91E-5		11	1.77E-5
13	SGTC_2956	9076196	3.97	2.5 µM	3.76E-5		15	3.54E-5
14	SGTC_1298	1079-1278	3.96	263.0 µM	4.00E-5	DNA damage response	16	3.77E-5
15	SGTC_2512	diallyl trisulfide	3.82	49.2 µM	6.88E-5		10	6.56E-5
16	SGTC_2037	5169046	3.73	200.0 µM	1.01E-4	60S ribosome export	16	9.67E-5
17	SGTC_1728	st035614	3.68	33.8 µM	1.22E-4		65	1.18E-4
18	SGTC_3169	9102970	3.59	49.5 µM	1.71E-4	RPP1 & pyrimidine depletion	9	1.66E-4
19	SGTC_838	0109-0203	3.58	71.4 µM	1.74E-4		26	1.69E-4
20	SGTC_1498	4478-3555	3.49	97.6 µM	2.44E-4		10	2.38E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.154	2.47E-19	YPL004C	LSP1	hom	Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.147	1.22E-17	YDR374C_p	YDR374C_p	hom	Putative protein of unknown function
0.142	1.22E-16	YDR179C	CSN9	hom	Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
0.134	6.72E-15	YNL218W	MGS1	hom	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.124	5.44E-13	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.121	1.77E-12	YGR242W_d	YGR242W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
0.119	4.81E-12	YNL242W	ATG2	hom	Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.118	5.84E-12	YOR155C	ISN1	hom	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.116	1.69E-11	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.111	1.11E-10	YKL172W	EBP2	het	Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.110	1.74E-10	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.110	1.88E-10	YKL201C	MNN4	hom	Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.109	2.15E-10	YKL161C	KDX1	hom	Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.109	2.65E-10	YMR080C	NAM7	hom	ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.108	3.51E-10	YKR009C	FOX2	hom	Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities