YKL171W / NNK1

Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL171W / NNK1

Click on Significant Values for Screen Details ID:SGTC_284|Compound:0988-0104|FD-Score:3.44|P-value:3.32E-5 ID:SGTC_326|Compound:k292-0812|FD-Score:3.71|P-value:8.37E-6 ID:SGTC_331|Compound:k048-0133|FD-Score:-2.88|P-value:4.07E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-2.78|P-value:6.17E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.71|P-value:8.14E-6 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.74|P-value:7.24E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-2.77|P-value:6.32E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-2.76|P-value:6.57E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:2.99|P-value:2.70E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.02|P-value:2.24E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.20|P-value:1.01E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.05|P-value:2.26E-9 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.76|P-value:6.76E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.40|P-value:3.93E-5 ID:SGTC_2103|Compound:5378138|FD-Score:3.54|P-value:2.02E-5 ID:SGTC_2125|Compound:5246583|FD-Score:4.44|P-value:1.31E-7 ID:SGTC_2130|Compound:NSC 7|FD-Score:2.99|P-value:2.67E-4 ID:SGTC_2316|Compound:5844804|FD-Score:2.96|P-value:3.00E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.76|P-value:6.50E-6 ID:SGTC_2359|Compound:9039622|FD-Score:2.80|P-value:5.86E-4 ID:SGTC_2375|Compound:9072683|FD-Score:2.87|P-value:4.47E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.97|P-value:2.10E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.87|P-value:3.38E-6 ID:SGTC_2452|Compound:5863136|FD-Score:-2.77|P-value:6.30E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.15|P-value:1.29E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.00|P-value:2.50E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.61|P-value:1.45E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.01|P-value:2.42E-4 ID:SGTC_2625|Compound:euphol|FD-Score:2.78|P-value:6.38E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.83|P-value:5.33E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-4.21|P-value:5.07E-7 ID:SGTC_3002|Compound:9075603|FD-Score:-2.91|P-value:3.51E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.01|P-value:2.28E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:3.44|P-value:3.32E-5 ID:SGTC_326|Compound:k292-0812|FD-Score:3.71|P-value:8.37E-6 ID:SGTC_331|Compound:k048-0133|FD-Score:-2.88|P-value:4.07E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-2.78|P-value:6.17E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.71|P-value:8.14E-6 ID:SGTC_630|Compound:4356-0227|FD-Score:-2.74|P-value:7.24E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-2.77|P-value:6.32E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:-2.76|P-value:6.57E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:2.99|P-value:2.70E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.02|P-value:2.24E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.20|P-value:1.01E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.05|P-value:2.26E-9 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.76|P-value:6.76E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.40|P-value:3.93E-5 ID:SGTC_2103|Compound:5378138|FD-Score:3.54|P-value:2.02E-5 ID:SGTC_2125|Compound:5246583|FD-Score:4.44|P-value:1.31E-7 ID:SGTC_2130|Compound:NSC 7|FD-Score:2.99|P-value:2.67E-4 ID:SGTC_2316|Compound:5844804|FD-Score:2.96|P-value:3.00E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.76|P-value:6.50E-6 ID:SGTC_2359|Compound:9039622|FD-Score:2.80|P-value:5.86E-4 ID:SGTC_2375|Compound:9072683|FD-Score:2.87|P-value:4.47E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.97|P-value:2.10E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.87|P-value:3.38E-6 ID:SGTC_2452|Compound:5863136|FD-Score:-2.77|P-value:6.30E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.15|P-value:1.29E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.00|P-value:2.50E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.61|P-value:1.45E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.01|P-value:2.42E-4 ID:SGTC_2625|Compound:euphol|FD-Score:2.78|P-value:6.38E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.83|P-value:5.33E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-4.21|P-value:5.07E-7 ID:SGTC_3002|Compound:9075603|FD-Score:-2.91|P-value:3.51E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.01|P-value:2.28E-4

Top fitness defect scores for YKL171W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2125 5246583 4.44 200.0 µM 1.31E-7 tubulin folding & SWR complex 31 4.47E-6
2 SGTC_2415 st077333 3.97 57.7 µM 2.10E-6 44 3.60E-5
3 SGTC_2329 6138490 3.76 200.0 µM 6.50E-6 39 8.42E-5
4 SGTC_326 k292-0812 3.71 17.5 µM 8.37E-6 10 1.02E-4
5 SGTC_2602 kawain 3.61 100.0 µM 1.45E-5 37 1.55E-4
6 SGTC_2103 5378138 3.54 10.0 µM 2.02E-5 26 1.98E-4
7 SGTC_284 0988-0104 3.44 32.6 µM 3.32E-5 heme requiring 19 2.88E-4
8 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.15 3.7 µM 1.29E-4 173 8.03E-4
9 SGTC_2621 lithochol-11-enic acid 3.01 100.0 µM 2.42E-4 78 0.00130
10 SGTC_2584 androsterone 3.00 48.7 µM 2.50E-4 cell wall 215 0.00133
11 SGTC_2130 NSC 7 2.99 2.5 µM 2.67E-4 copper-dependent oxidative stress 88 0.00140
12 SGTC_882 0843-0131 2.99 351.0 µM 2.70E-4 56 0.00141
13 SGTC_2316 5844804 2.96 197.4 µM 3.00E-4 99 0.00153
14 SGTC_2375 9072683 2.87 200.0 µM 4.47E-4 mitochondrial processes 76 0.00207
15 SGTC_2791 5663188 2.83 71.4 µM 5.33E-4 RPP1 & pyrimidine depletion 73 0.00236
16 SGTC_2359 9039622 2.80 200.0 µM 5.86E-4 endomembrane recycling 87 0.00254
17 SGTC_2625 euphol 2.78 100.0 µM 6.38E-4 111 0.00271
18 SGTC_436 n,n-dimethylsphingosine 2.64 3.3 µM 0.00110 108 0.00410
19 SGTC_2303 7774051 2.64 200.0 µM 0.00114 87 0.00420
20 SGTC_280 3448-3607 2.61 3.0 µM 0.00123 60S ribosome export 60 0.00448

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.334 4.41E-88 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.292 7.09E-67 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.281 9.60E-62 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.268 3.75E-56 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.267 7.81E-56 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.263 2.18E-54 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.240 2.76E-45 YGL157W ARI1 hom NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
0.240 4.05E-45 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.234 4.06E-43 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.228 5.80E-41 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.224 1.60E-39 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.220 4.73E-38 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.218 2.66E-37 YDR139C RUB1 hom Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
0.213 9.15E-36 YBL066C SEF1 hom Putative transcription factor, has homolog in Kluyveromyces lactis
0.211 5.51E-35 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication