HIPHOP chemogenomics database

YKL176C / LST4

Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL176C / LST4

Click on Significant Values for Screen Details

Top fitness defect scores for YKL176C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_690	glyoxal	11.80	20.3 µM	5.55E-28		13	1.63E-32
2	SGTC_138	0850-0259	11.20	40.9 µM	3.52E-25	cell wall	34	3.09E-29
3	SGTC_2688	kanamycin b	10.50	96.7 µM	1.44E-22	calcium & mitochondrial duress	22	3.51E-26
4	SGTC_1754	st042065	9.81	16.1 µM	7.07E-20	NEO1-PIK1	30	4.94E-23
5	SGTC_87	0100-0052	7.25	35.5 µM	1.21E-11		19	2.10E-13
6	SGTC_1957	st077035	7.15	62.4 µM	2.23E-11		2	4.32E-13
7	SGTC_1781	st038725	7.01	66.3 µM	5.25E-11	NEO1	39	1.17E-12
8	SGTC_1712	st032288	5.98	53.4 µM	1.82E-8	calcium & mitochondrial duress	77	1.10E-9
9	SGTC_2912	7964298	5.96	10.9 µM	2.03E-8	cell wall	46	1.24E-9
10	SGTC_1119	parthenolide	5.92	318.5 nM	2.48E-8	TSC3-RPN4	11	1.58E-9
11	SGTC_1077	5-fluorocytosine	5.68	377.0 nM	8.47E-8	exosome	2	6.61E-9
12	SGTC_1019	4262-0212	5.60	3.4 µM	1.26E-7	ergosterol depletion effects on membrane	28	1.06E-8
13	SGTC_3311	9131146	5.58	2.9 µM	1.45E-7	cell wall	66	1.24E-8
14	SGTC_1503	4585-0012	5.30	61.3 µM	5.44E-7		2	5.81E-8
15	SGTC_1597	7-benzyloxygramine	5.11	47.5 µM	1.29E-6	calcium & mitochondrial duress	65	1.59E-7
16	SGTC_1975	st070967	4.58	30.8 µM	1.28E-5		23	2.33E-6
17	SGTC_770	0389-1121	4.16	67.2 µM	6.66E-5	RPP1 & pyrimidine depletion	22	1.59E-5
18	SGTC_667	1498-1044	4.05	59.0 µM	1.01E-4	RPP1 & pyrimidine depletion	15	2.58E-5
19	SGTC_820	1582-0060	4.01	59.9 µM	1.16E-4		29	3.05E-5
20	SGTC_833	3852-0053	4.00	558.0 µM	1.22E-4		15	3.22E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.365	3.18E-106	YGR057C	LST7	hom	Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.282	1.59E-62	YCR075W-A_p	YCR075W-A_p	hom	Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.267	4.56E-56	YIL052C	RPL34B	hom	Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.255	7.71E-51	YBL051C	PIN4	hom	Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.248	3.43E-48	YGL100W	SEH1	hom	Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
0.245	3.62E-47	YGR163W	GTR2	hom	Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.241	1.70E-45	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.237	5.53E-44	YKR007W	MEH1	hom	Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
0.224	2.18E-39	YJR111C_p	YJR111C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.221	2.05E-38	YDR417C_d	YDR417C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.210	1.56E-34	YBR077C	SLM4	hom	Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.206	1.30E-33	YNL307C	MCK1	hom	Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.203	1.70E-32	YJL179W	PFD1	hom	Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
0.195	3.45E-30	YBL006C	LDB7	hom	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.195	4.52E-30	YNL070W	TOM7	hom	Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex