YKL176C / LST4

Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL176C / LST4

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:3.92|P-value:1.61E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:7.25|P-value:1.21E-11 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.74|P-value:2.43E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:11.20|P-value:3.52E-25 ID:SGTC_163|Compound:1327-0036|FD-Score:3.43|P-value:8.31E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:3.64|P-value:4.14E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:-4.00|P-value:9.68E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.87|P-value:1.54E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.74|P-value:3.00E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.85|P-value:1.56E-13 ID:SGTC_393|Compound:acivicin|FD-Score:-5.35|P-value:3.27E-7 ID:SGTC_582|Compound:0987-0078|FD-Score:3.39|P-value:9.32E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.95|P-value:1.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.47|P-value:7.33E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.86|P-value:1.95E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.05|P-value:1.01E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:11.80|P-value:5.55E-28 ID:SGTC_724|Compound:3910-0327|FD-Score:3.47|P-value:7.39E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.56|P-value:5.54E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.41|P-value:8.82E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.16|P-value:6.66E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:4.01|P-value:1.16E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.00|P-value:1.22E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:5.60|P-value:1.26E-7 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.68|P-value:8.47E-8 ID:SGTC_1119|Compound:parthenolide|FD-Score:5.92|P-value:2.48E-8 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.50|P-value:6.64E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.69|P-value:3.57E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.58|P-value:4.14E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:5.30|P-value:5.44E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-4.87|P-value:2.89E-6 ID:SGTC_1526|Compound:st051518|FD-Score:-4.14|P-value:5.64E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.11|P-value:1.29E-6 ID:SGTC_1712|Compound:st032288|FD-Score:5.98|P-value:1.82E-8 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.97|P-value:1.07E-4 ID:SGTC_1754|Compound:st042065|FD-Score:9.81|P-value:7.07E-20 ID:SGTC_1781|Compound:st038725|FD-Score:7.01|P-value:5.25E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.79|P-value:2.53E-4 ID:SGTC_1957|Compound:st077035|FD-Score:7.15|P-value:2.23E-11 ID:SGTC_1958|Compound:st076513|FD-Score:-6.21|P-value:3.78E-9 ID:SGTC_1966|Compound:st070145|FD-Score:-4.90|P-value:2.56E-6 ID:SGTC_1968|Compound:st069590|FD-Score:-4.00|P-value:9.80E-5 ID:SGTC_1975|Compound:st070967|FD-Score:4.58|P-value:1.28E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.83|P-value:2.16E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.48|P-value:1.50E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-3.57|P-value:4.34E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.36|P-value:8.64E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.93|P-value:1.52E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:10.50|P-value:1.44E-22 ID:SGTC_2692|Compound:st077426|FD-Score:-3.31|P-value:9.85E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.84|P-value:2.15E-4 ID:SGTC_2912|Compound:7964298|FD-Score:5.96|P-value:2.03E-8 ID:SGTC_2937|Compound:9044753|FD-Score:3.46|P-value:7.45E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.06|P-value:7.83E-5 ID:SGTC_3311|Compound:9131146|FD-Score:5.58|P-value:1.45E-7 ID:SGTC_59|Compound:0330-0160|FD-Score:3.92|P-value:1.61E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:7.25|P-value:1.21E-11 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.74|P-value:2.43E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:11.20|P-value:3.52E-25 ID:SGTC_163|Compound:1327-0036|FD-Score:3.43|P-value:8.31E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:3.64|P-value:4.14E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:-4.00|P-value:9.68E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.87|P-value:1.54E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.74|P-value:3.00E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.85|P-value:1.56E-13 ID:SGTC_393|Compound:acivicin|FD-Score:-5.35|P-value:3.27E-7 ID:SGTC_582|Compound:0987-0078|FD-Score:3.39|P-value:9.32E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.95|P-value:1.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.47|P-value:7.33E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.86|P-value:1.95E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.05|P-value:1.01E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:11.80|P-value:5.55E-28 ID:SGTC_724|Compound:3910-0327|FD-Score:3.47|P-value:7.39E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.56|P-value:5.54E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.41|P-value:8.82E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.16|P-value:6.66E-5 ID:SGTC_820|Compound:1582-0060|FD-Score:4.01|P-value:1.16E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.00|P-value:1.22E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:5.60|P-value:1.26E-7 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:5.68|P-value:8.47E-8 ID:SGTC_1119|Compound:parthenolide|FD-Score:5.92|P-value:2.48E-8 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.50|P-value:6.64E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.69|P-value:3.57E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.58|P-value:4.14E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:5.30|P-value:5.44E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-4.87|P-value:2.89E-6 ID:SGTC_1526|Compound:st051518|FD-Score:-4.14|P-value:5.64E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.11|P-value:1.29E-6 ID:SGTC_1712|Compound:st032288|FD-Score:5.98|P-value:1.82E-8 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.97|P-value:1.07E-4 ID:SGTC_1754|Compound:st042065|FD-Score:9.81|P-value:7.07E-20 ID:SGTC_1781|Compound:st038725|FD-Score:7.01|P-value:5.25E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.79|P-value:2.53E-4 ID:SGTC_1957|Compound:st077035|FD-Score:7.15|P-value:2.23E-11 ID:SGTC_1958|Compound:st076513|FD-Score:-6.21|P-value:3.78E-9 ID:SGTC_1966|Compound:st070145|FD-Score:-4.90|P-value:2.56E-6 ID:SGTC_1968|Compound:st069590|FD-Score:-4.00|P-value:9.80E-5 ID:SGTC_1975|Compound:st070967|FD-Score:4.58|P-value:1.28E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.83|P-value:2.16E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-4.48|P-value:1.50E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-3.57|P-value:4.34E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.36|P-value:8.64E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.93|P-value:1.52E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:10.50|P-value:1.44E-22 ID:SGTC_2692|Compound:st077426|FD-Score:-3.31|P-value:9.85E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.84|P-value:2.15E-4 ID:SGTC_2912|Compound:7964298|FD-Score:5.96|P-value:2.03E-8 ID:SGTC_2937|Compound:9044753|FD-Score:3.46|P-value:7.45E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-4.06|P-value:7.83E-5 ID:SGTC_3311|Compound:9131146|FD-Score:5.58|P-value:1.45E-7

Top fitness defect scores for YKL176C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 11.80 20.3 µM 5.55E-28 13 1.63E-32
2 SGTC_138 0850-0259 11.20 40.9 µM 3.52E-25 cell wall 34 3.09E-29
3 SGTC_2688 kanamycin b 10.50 96.7 µM 1.44E-22 calcium & mitochondrial duress 22 3.51E-26
4 SGTC_1754 st042065 9.81 16.1 µM 7.07E-20 NEO1-PIK1 30 4.94E-23
5 SGTC_87 0100-0052 7.25 35.5 µM 1.21E-11 19 2.10E-13
6 SGTC_1957 st077035 7.15 62.4 µM 2.23E-11 2 4.32E-13
7 SGTC_1781 st038725 7.01 66.3 µM 5.25E-11 NEO1 39 1.17E-12
8 SGTC_1712 st032288 5.98 53.4 µM 1.82E-8 calcium & mitochondrial duress 77 1.10E-9
9 SGTC_2912 7964298 5.96 10.9 µM 2.03E-8 cell wall 46 1.24E-9
10 SGTC_1119 parthenolide 5.92 318.5 nM 2.48E-8 TSC3-RPN4 11 1.58E-9
11 SGTC_1077 5-fluorocytosine 5.68 377.0 nM 8.47E-8 exosome 2 6.61E-9
12 SGTC_1019 4262-0212 5.60 3.4 µM 1.26E-7 ergosterol depletion effects on membrane 28 1.06E-8
13 SGTC_3311 9131146 5.58 2.9 µM 1.45E-7 cell wall 66 1.24E-8
14 SGTC_1503 4585-0012 5.30 61.3 µM 5.44E-7 2 5.81E-8
15 SGTC_1597 7-benzyloxygramine 5.11 47.5 µM 1.29E-6 calcium & mitochondrial duress 65 1.59E-7
16 SGTC_1975 st070967 4.58 30.8 µM 1.28E-5 23 2.33E-6
17 SGTC_770 0389-1121 4.16 67.2 µM 6.66E-5 RPP1 & pyrimidine depletion 22 1.59E-5
18 SGTC_667 1498-1044 4.05 59.0 µM 1.01E-4 RPP1 & pyrimidine depletion 15 2.58E-5
19 SGTC_820 1582-0060 4.01 59.9 µM 1.16E-4 29 3.05E-5
20 SGTC_833 3852-0053 4.00 558.0 µM 1.22E-4 15 3.22E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.365 3.18E-106 YGR057C LST7 hom Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.282 1.59E-62 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.267 4.56E-56 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.255 7.71E-51 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.248 3.43E-48 YGL100W SEH1 hom Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
0.245 3.62E-47 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.241 1.70E-45 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.237 5.53E-44 YKR007W MEH1 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
0.224 2.18E-39 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.221 2.05E-38 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.210 1.56E-34 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.206 1.30E-33 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.203 1.70E-32 YJL179W PFD1 hom Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
0.195 3.45E-30 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.195 4.52E-30 YNL070W TOM7 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex