YKL191W / DPH2

Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL191W / DPH2

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.25|P-value:2.56E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:3.32|P-value:1.94E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:3.07|P-value:5.17E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.65|P-value:4.77E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.31|P-value:2.11E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.37|P-value:4.86E-12 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.11|P-value:4.49E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.67|P-value:4.30E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.26|P-value:2.41E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.23|P-value:2.74E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.29|P-value:2.25E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.04|P-value:5.67E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.57|P-value:6.73E-5 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.79|P-value:2.68E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:2.89|P-value:9.92E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.82|P-value:2.23E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:4.22|P-value:3.18E-6 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.47|P-value:1.07E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.00|P-value:6.51E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.52|P-value:8.14E-5 ID:SGTC_1743|Compound:st037606|FD-Score:-3.50|P-value:9.50E-5 ID:SGTC_1745|Compound:st081495|FD-Score:5.17|P-value:1.65E-8 ID:SGTC_1750|Compound:st081859|FD-Score:6.60|P-value:8.24E-13 ID:SGTC_1754|Compound:st042065|FD-Score:4.96|P-value:5.65E-8 ID:SGTC_1760|Compound:st045453|FD-Score:5.96|P-value:9.26E-11 ID:SGTC_1800|Compound:st050079|FD-Score:4.51|P-value:7.19E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.02|P-value:8.65E-6 ID:SGTC_1803|Compound:st050068|FD-Score:6.33|P-value:6.36E-12 ID:SGTC_1861|Compound:st057647|FD-Score:3.49|P-value:9.53E-5 ID:SGTC_1886|Compound:st057356|FD-Score:2.92|P-value:8.87E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.13|P-value:5.00E-6 ID:SGTC_1941|Compound:st076221|FD-Score:3.17|P-value:3.50E-4 ID:SGTC_2198|Compound:6589944|FD-Score:2.91|P-value:9.23E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.00|P-value:6.87E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.92|P-value:1.37E-5 ID:SGTC_2443|Compound:5493264|FD-Score:3.20|P-value:3.07E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.20|P-value:3.52E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.08|P-value:5.08E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:2.90|P-value:9.61E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-2.91|P-value:9.45E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.02|P-value:6.20E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.51|P-value:1.72E-12 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.62|P-value:5.28E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:8.11|P-value:2.25E-18 ID:SGTC_2802|Compound:7925815|FD-Score:3.20|P-value:3.08E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.26|P-value:2.44E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.12|P-value:4.28E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.54|P-value:7.77E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.21|P-value:2.99E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.50|P-value:8.93E-5 ID:SGTC_7|Compound:0192-1582|FD-Score:3.25|P-value:2.56E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:3.32|P-value:1.94E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:3.07|P-value:5.17E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.65|P-value:4.77E-5 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.31|P-value:2.11E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.37|P-value:4.86E-12 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.11|P-value:4.49E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.67|P-value:4.30E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.26|P-value:2.41E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.23|P-value:2.74E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.29|P-value:2.25E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.04|P-value:5.67E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.57|P-value:6.73E-5 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.79|P-value:2.68E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:2.89|P-value:9.92E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.82|P-value:2.23E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:4.22|P-value:3.18E-6 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.47|P-value:1.07E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.00|P-value:6.51E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.52|P-value:8.14E-5 ID:SGTC_1743|Compound:st037606|FD-Score:-3.50|P-value:9.50E-5 ID:SGTC_1745|Compound:st081495|FD-Score:5.17|P-value:1.65E-8 ID:SGTC_1750|Compound:st081859|FD-Score:6.60|P-value:8.24E-13 ID:SGTC_1754|Compound:st042065|FD-Score:4.96|P-value:5.65E-8 ID:SGTC_1760|Compound:st045453|FD-Score:5.96|P-value:9.26E-11 ID:SGTC_1800|Compound:st050079|FD-Score:4.51|P-value:7.19E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.02|P-value:8.65E-6 ID:SGTC_1803|Compound:st050068|FD-Score:6.33|P-value:6.36E-12 ID:SGTC_1861|Compound:st057647|FD-Score:3.49|P-value:9.53E-5 ID:SGTC_1886|Compound:st057356|FD-Score:2.92|P-value:8.87E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.13|P-value:5.00E-6 ID:SGTC_1941|Compound:st076221|FD-Score:3.17|P-value:3.50E-4 ID:SGTC_2198|Compound:6589944|FD-Score:2.91|P-value:9.23E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.00|P-value:6.87E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:3.92|P-value:1.37E-5 ID:SGTC_2443|Compound:5493264|FD-Score:3.20|P-value:3.07E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.20|P-value:3.52E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.08|P-value:5.08E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:2.90|P-value:9.61E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-2.91|P-value:9.45E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.02|P-value:6.20E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.51|P-value:1.72E-12 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.62|P-value:5.28E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:8.11|P-value:2.25E-18 ID:SGTC_2802|Compound:7925815|FD-Score:3.20|P-value:3.08E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.26|P-value:2.44E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.12|P-value:4.28E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.54|P-value:7.77E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.21|P-value:2.99E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.50|P-value:8.93E-5

Top fitness defect scores for YKL191W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 8.11 5.0 µM 2.25E-18 35 2.63E-16
2 SGTC_1750 st081859 6.60 2.4 µM 8.24E-13 endomembrane recycling 95 2.00E-11
3 SGTC_2638 epoxomicin 6.51 18.0 µM 1.72E-12 5 3.81E-11
4 SGTC_221 trifluoperazine 6.37 10.3 µM 4.86E-12 NEO1-PIK1 114 9.49E-11
5 SGTC_1803 st050068 6.33 52.4 µM 6.36E-12 NEO1-PIK1 73 1.20E-10
6 SGTC_1760 st045453 5.96 10.4 µM 9.26E-11 NEO1-PIK1 82 1.26E-9
7 SGTC_1745 st081495 5.17 74.0 µM 1.65E-8 RNA processing & uracil transport 37 1.20E-7
8 SGTC_1754 st042065 4.96 16.1 µM 5.65E-8 NEO1-PIK1 127 3.53E-7
9 SGTC_1800 st050079 4.51 29.1 µM 7.19E-7 NEO1-PIK1 94 3.30E-6
10 SGTC_1200 1643-0193 4.22 18.4 µM 3.18E-6 10 1.22E-5
11 SGTC_2573 2',4'-dihydroxychalcone 4.20 15.0 µM 3.52E-6 41 1.33E-5
12 SGTC_1888 azatryptophan 4.13 97.5 µM 5.00E-6 12 1.82E-5
13 SGTC_1801 st050089 4.02 28.4 µM 8.65E-6 NEO1-PIK1 115 2.95E-5
14 SGTC_2407 paclitaxel 3.92 400.0 µM 1.37E-5 36 4.42E-5
15 SGTC_1106 tegaserod maleate 3.82 11.7 µM 2.23E-5 22 6.79E-5
16 SGTC_361 000s-0510 3.67 1.4 µM 4.30E-5 38 1.21E-4
17 SGTC_122 0236-0016 3.65 402.4 µM 4.77E-5 RNA processing & uracil transport 44 1.33E-4
18 SGTC_2640 pentamidine 3.62 10.4 µM 5.28E-5 mitochondrial processes 42 1.45E-4
19 SGTC_649 1123-0311 3.57 367.0 µM 6.73E-5 endomembrane recycling 89 1.80E-4
20 SGTC_3125 9127534 3.54 49.5 µM 7.77E-5 19 2.04E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.439 2.39E-158 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.343 3.57E-93 YML106W URA5 hom Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
0.340 8.69E-92 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.317 2.59E-79 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.316 1.96E-78 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.311 1.06E-75 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.303 2.95E-72 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.300 6.45E-71 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.294 5.32E-68 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.287 7.30E-65 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.287 1.05E-64 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.282 1.90E-62 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.279 3.05E-61 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.279 4.94E-61 YKR072C SIS2 hom Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
0.274 4.78E-59 YJR097W JJJ3 hom Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4