HIPHOP chemogenomics database

YKL194C / MST1

Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL194C / MST1

Click on Significant Values for Screen Details

Top fitness defect scores for YKL194C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_386	1509-0011	36.90	3.1 µM	3.54E-207		2	8.35E-298
2	SGTC_236	1509-0011	29.30	5.3 µM	2.38E-131		17	1.88E-188
3	SGTC_1635	st007035	29.00	42.4 µM	2.12E-129		8	1.21E-185
4	SGTC_275	trichlorophene	11.70	5.7 µM	1.15E-22	mitochondrial stress	25	7.76E-32
5	SGTC_134	0269-0018	8.60	6.9 µM	3.89E-13		53	3.82E-18
6	SGTC_2980	9014342	8.32	25.3 µM	2.17E-12		8	4.49E-17
7	SGTC_3218	9131285	8.14	49.5 µM	6.06E-12	endomembrane recycling	8	1.96E-16
8	SGTC_1528	arabinosylhypoxanthine	7.74	74.6 µM	5.69E-11		3	4.86E-15
9	SGTC_2811	7967822	6.60	32.5 µM	1.98E-8		2	2.10E-11
10	SGTC_2369	9071472	6.55	200.0 µM	2.50E-8		1	2.94E-11
11	SGTC_149	3970-0822	6.37	46.1 µM	5.60E-8	tubulin folding & SWR complex	31	9.33E-11
12	SGTC_70	0141-0289	6.16	43.3 µM	1.44E-7	mitochondrial stress	77	3.60E-10
13	SGTC_173	1273-0061	5.96	2.7 µM	3.48E-7	mitochondrial stress	108	1.27E-9
14	SGTC_1742	st038235	5.71	28.9 µM	9.82E-7	mitochondrial stress	52	5.56E-9
15	SGTC_1883	5-benzylidenerhodanine	5.64	20.0 µM	1.31E-6	TRP & mitochondrial translation	21	8.43E-9
16	SGTC_908	3448-7491	5.44	47.7 µM	2.93E-6	mitochondrial stress	61	2.64E-8
17	SGTC_1122	capsaicin	5.35	818.6 nM	4.17E-6		8	4.37E-8
18	SGTC_157	1348-1695	5.03	65.8 µM	1.39E-5	iron homeostasis	33	2.42E-7
19	SGTC_2472	5377652	4.96	49.4 µM	1.79E-5	TRP & mitochondrial translation	32	3.48E-7
20	SGTC_2693	st077581	4.83	40.7 µM	2.89E-5		15	6.85E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	ORF	Gene	Zygosity	Description
0.706	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.705	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.680	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.652	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.644	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.641	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.634	YHR116W	COX23	hom	Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.631	YPL132W	COX11	hom	Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.628	YKL155C	RSM22	hom	Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.623	YDR231C	COX20	hom	Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.621	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.619	YLL009C	COX17	hom	Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.616	YDR518W	EUG1	hom	Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.613	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.609	YNL213C	RRG9	hom	Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies