YKL215C / OXP1

5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL215C / OXP1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.75|P-value:3.27E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.23|P-value:6.32E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-4.18|P-value:1.34E-5 ID:SGTC_282|Compound:k297-0036|FD-Score:3.02|P-value:6.94E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:2.99|P-value:7.60E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.73|P-value:4.27E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.92|P-value:4.24E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.23|P-value:6.31E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.20|P-value:7.05E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:3.05|P-value:6.17E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.26|P-value:3.87E-6 ID:SGTC_966|Compound:1300-0360|FD-Score:3.12|P-value:4.75E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:3.23|P-value:3.15E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:2.92|P-value:9.73E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.48|P-value:2.51E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.29|P-value:5.09E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:-3.47|P-value:2.63E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:-5.43|P-value:2.10E-8 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.84|P-value:5.75E-5 ID:SGTC_1516|Compound:st013856|FD-Score:3.12|P-value:4.80E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.12|P-value:9.30E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.89|P-value:2.10E-5 ID:SGTC_1628|Compound:st004252|FD-Score:4.24|P-value:4.29E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.66|P-value:1.21E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.77|P-value:3.55E-5 ID:SGTC_1819|Compound:st053448|FD-Score:5.43|P-value:6.89E-9 ID:SGTC_1954|Compound:st077032|FD-Score:2.94|P-value:9.31E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.26|P-value:2.91E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.99|P-value:7.79E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.17|P-value:3.94E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.51|P-value:2.24E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.67|P-value:5.56E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.61|P-value:7.14E-5 ID:SGTC_2763|Compound:lisinopril|FD-Score:-4.61|P-value:1.68E-6 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.89|P-value:4.82E-5 ID:SGTC_2857|Compound:9024691|FD-Score:3.13|P-value:4.58E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.55|P-value:8.96E-7 ID:SGTC_2972|Compound:9093619|FD-Score:-3.26|P-value:5.76E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.28|P-value:5.27E-4 ID:SGTC_3154|Compound:9100113|FD-Score:3.24|P-value:3.04E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.48|P-value:1.18E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.75|P-value:3.27E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.23|P-value:6.32E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-4.18|P-value:1.34E-5 ID:SGTC_282|Compound:k297-0036|FD-Score:3.02|P-value:6.94E-4 ID:SGTC_399|Compound:oxolinic acid|FD-Score:2.99|P-value:7.60E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.73|P-value:4.27E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:-3.92|P-value:4.24E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.23|P-value:6.31E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.20|P-value:7.05E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:3.05|P-value:6.17E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.26|P-value:3.87E-6 ID:SGTC_966|Compound:1300-0360|FD-Score:3.12|P-value:4.75E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:3.23|P-value:3.15E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:2.92|P-value:9.73E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.48|P-value:2.51E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-3.29|P-value:5.09E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:-3.47|P-value:2.63E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:-5.43|P-value:2.10E-8 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.84|P-value:5.75E-5 ID:SGTC_1516|Compound:st013856|FD-Score:3.12|P-value:4.80E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.12|P-value:9.30E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.89|P-value:2.10E-5 ID:SGTC_1628|Compound:st004252|FD-Score:4.24|P-value:4.29E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.66|P-value:1.21E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.77|P-value:3.55E-5 ID:SGTC_1819|Compound:st053448|FD-Score:5.43|P-value:6.89E-9 ID:SGTC_1954|Compound:st077032|FD-Score:2.94|P-value:9.31E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.26|P-value:2.91E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.99|P-value:7.79E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.17|P-value:3.94E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.51|P-value:2.24E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.67|P-value:5.56E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.61|P-value:7.14E-5 ID:SGTC_2763|Compound:lisinopril|FD-Score:-4.61|P-value:1.68E-6 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.89|P-value:4.82E-5 ID:SGTC_2857|Compound:9024691|FD-Score:3.13|P-value:4.58E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.55|P-value:8.96E-7 ID:SGTC_2972|Compound:9093619|FD-Score:-3.26|P-value:5.76E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.28|P-value:5.27E-4 ID:SGTC_3154|Compound:9100113|FD-Score:3.24|P-value:3.04E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.48|P-value:1.18E-4

Top fitness defect scores for YKL215C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1819 st053448 5.43 58.6 µM 6.89E-9 amide catabolism 5 2.81E-8
2 SGTC_6 0844-0013 4.75 19.8 µM 3.27E-7 49 1.03E-6
3 SGTC_2947 9072639 4.55 7.7 µM 8.96E-7 tubulin folding & SWR complex 28 2.63E-6
4 SGTC_962 1254-0300 4.26 21.5 µM 3.87E-6 15 1.02E-5
5 SGTC_1628 st004252 4.24 77.7 µM 4.29E-6 14 1.13E-5
6 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 3.89 54.9 µM 2.10E-5 redox potentiating 57 4.94E-5
7 SGTC_1816 st051957 3.77 91.6 µM 3.55E-5 12 8.04E-5
8 SGTC_440 fluspirilene 3.73 35.1 µM 4.27E-5 20 9.56E-5
9 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.67 3.7 µM 5.56E-5 105 1.22E-4
10 SGTC_2538 3',7'-epoxycaryophyllan-6-one 3.61 100.0 µM 7.14E-5 34 1.54E-4
11 SGTC_3156 9099931 3.48 49.5 µM 1.18E-4 19 2.46E-4
12 SGTC_1993 st073995 3.26 70.8 µM 2.91E-4 39 5.66E-4
13 SGTC_3154 9100113 3.24 49.5 µM 3.04E-4 60 5.90E-4
14 SGTC_970 1315-0089 3.23 19.5 µM 3.15E-4 RSC & ERG11 50 6.10E-4
15 SGTC_2131 toluylene red 3.17 5.3 µM 3.94E-4 60S ribosome export 61 7.51E-4
16 SGTC_2857 9024691 3.13 58.4 µM 4.58E-4 59 8.63E-4
17 SGTC_966 1300-0360 3.12 30.2 µM 4.75E-4 heme requiring 59 8.93E-4
18 SGTC_1516 st013856 3.12 110.0 µM 4.80E-4 33 9.01E-4
19 SGTC_861 0443-0273 3.05 110.0 µM 6.17E-4 45 0.00114
20 SGTC_282 k297-0036 3.02 65.7 µM 6.94E-4 21 0.00127

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.206 2.15E-33 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.180 8.14E-26 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.157 7.68E-20 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.157 7.73E-20 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.152 6.89E-19 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.139 5.63E-16 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.133 9.51E-15 YOR173W DCS2 hom Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance
0.125 3.81E-13 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.121 1.80E-12 YDR153C ENT5 hom Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
0.121 1.91E-12 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.119 4.20E-12 YGR040W KSS1 hom Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
0.119 4.42E-12 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.114 4.09E-11 YKR039W GAP1 hom General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
0.113 4.68E-11 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.111 1.18E-10 YGR011W_d YGR011W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data