YKL217W / JEN1

Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL217W / JEN1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.55|P-value:2.26E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:-4.08|P-value:4.34E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:-3.45|P-value:4.73E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.98|P-value:4.41E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-5.47|P-value:6.66E-8 ID:SGTC_296|Compound:1222-0039|FD-Score:4.22|P-value:1.57E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-5.08|P-value:4.95E-7 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.45|P-value:1.37E-13 ID:SGTC_480|Compound:am 92016|FD-Score:3.26|P-value:6.28E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.67|P-value:1.43E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.58|P-value:2.05E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.22|P-value:1.62E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.35|P-value:4.67E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.26|P-value:6.38E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-4.34|P-value:1.49E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-3.72|P-value:1.80E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-5.57|P-value:3.82E-8 ID:SGTC_1636|Compound:st007707|FD-Score:-7.32|P-value:7.47E-13 ID:SGTC_1637|Compound:st007924|FD-Score:8.73|P-value:5.96E-18 ID:SGTC_1646|Compound:st011709|FD-Score:3.45|P-value:3.23E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.66|P-value:1.53E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-3.43|P-value:5.09E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.30|P-value:5.56E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.73|P-value:1.15E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.65|P-value:1.57E-4 ID:SGTC_2134|Compound:5324836|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.57|P-value:2.12E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-4.25|P-value:2.21E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-3.85|P-value:1.10E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.75|P-value:1.48E-6 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.51|P-value:2.66E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.47|P-value:3.04E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.21|P-value:7.41E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.32|P-value:5.23E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:5.27|P-value:1.12E-7 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.72|P-value:9.62E-9 ID:SGTC_2819|Compound:7996655|FD-Score:-4.52|P-value:6.85E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-6.66|P-value:6.26E-11 ID:SGTC_6|Compound:0844-0013|FD-Score:3.55|P-value:2.26E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:-4.08|P-value:4.34E-5 ID:SGTC_31|Compound:0508-1974|FD-Score:-3.45|P-value:4.73E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.98|P-value:4.41E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-5.47|P-value:6.66E-8 ID:SGTC_296|Compound:1222-0039|FD-Score:4.22|P-value:1.57E-5 ID:SGTC_407|Compound:eburnamonine|FD-Score:-5.08|P-value:4.95E-7 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.45|P-value:1.37E-13 ID:SGTC_480|Compound:am 92016|FD-Score:3.26|P-value:6.28E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.67|P-value:1.43E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.58|P-value:2.05E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.22|P-value:1.62E-5 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.35|P-value:4.67E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.26|P-value:6.38E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-4.34|P-value:1.49E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-3.72|P-value:1.80E-4 ID:SGTC_1635|Compound:st007035|FD-Score:-5.57|P-value:3.82E-8 ID:SGTC_1636|Compound:st007707|FD-Score:-7.32|P-value:7.47E-13 ID:SGTC_1637|Compound:st007924|FD-Score:8.73|P-value:5.96E-18 ID:SGTC_1646|Compound:st011709|FD-Score:3.45|P-value:3.23E-4 ID:SGTC_1648|Compound:st011736|FD-Score:3.66|P-value:1.53E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-3.43|P-value:5.09E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.30|P-value:5.56E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.73|P-value:1.15E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.65|P-value:1.57E-4 ID:SGTC_2134|Compound:5324836|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.57|P-value:2.12E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-4.25|P-value:2.21E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-3.85|P-value:1.10E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.75|P-value:1.48E-6 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.51|P-value:2.66E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.47|P-value:3.04E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.21|P-value:7.41E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.32|P-value:5.23E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:5.27|P-value:1.12E-7 ID:SGTC_2776|Compound:terbinafine|FD-Score:5.72|P-value:9.62E-9 ID:SGTC_2819|Compound:7996655|FD-Score:-4.52|P-value:6.85E-6 ID:SGTC_3210|Compound:9128157|FD-Score:-6.66|P-value:6.26E-11

Top fitness defect scores for YKL217W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1637 st007924 8.73 17.4 µM 5.96E-18 2 1.22E-18
2 SGTC_423 5-fluorouracil 7.45 457.0 µM 1.37E-13 exosome 1 4.54E-14
3 SGTC_2776 terbinafine 5.72 19.9 µM 9.62E-9 cell wall signaling 23 5.33E-9
4 SGTC_2772 vinpocetine 5.27 57.1 µM 1.12E-7 7 6.90E-8
5 SGTC_2414 st077232 4.75 92.9 µM 1.48E-6 11 1.03E-6
6 SGTC_296 1222-0039 4.22 74.9 µM 1.57E-5 9 1.20E-5
7 SGTC_1195 1502-0147 4.22 41.1 µM 1.62E-5 18 1.23E-5
8 SGTC_48 0382-0018 3.98 55.0 µM 4.41E-5 8 3.51E-5
9 SGTC_1367 1812-0510 3.76 76.9 µM 1.02E-4 26 8.37E-5
10 SGTC_2134 5324836 3.76 1.5 µM 1.04E-4 19 8.53E-5
11 SGTC_1930 st074634 3.73 34.3 µM 1.15E-4 RNA pol III & RNase P/MRP 44 9.54E-5
12 SGTC_598 3937-0236 3.67 20.2 µM 1.43E-4 ERG2 37 1.19E-4
13 SGTC_1648 st011736 3.66 69.8 µM 1.53E-4 PDR1 21 1.27E-4
14 SGTC_2005 4024620 3.65 188.0 µM 1.57E-4 29 1.31E-4
15 SGTC_779 1360-0071 3.58 233.0 µM 2.05E-4 fatty acid desaturase (OLE1) 47 1.73E-4
16 SGTC_2265 7929201 3.57 200.0 µM 2.12E-4 28 1.80E-4
17 SGTC_6 0844-0013 3.55 19.8 µM 2.26E-4 133 1.91E-4
18 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.51 3.7 µM 2.66E-4 126 2.27E-4
19 SGTC_2498 4'-Methoxychalcone 3.47 54.6 µM 3.04E-4 RPP1 & pyrimidine depletion 49 2.61E-4
20 SGTC_1646 st011709 3.45 75.6 µM 3.23E-4 23 2.78E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 9.23E-24 YLL055W YCT1 hom High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
0.139 8.40E-16 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.138 1.15E-15 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.129 6.58E-14 YDR341C YDR341C het Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.121 1.88E-12 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.117 1.14E-11 YGR175C ERG1 het Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
0.114 4.19E-11 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.111 9.91E-11 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.102 3.23E-9 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.098 1.53E-8 YAL005C SSA1 hom ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
0.097 1.98E-8 YBL054W TOD6 hom PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication
0.093 6.50E-8 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.093 6.52E-8 YHR043C DOG2 hom 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed
0.093 7.20E-8 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
0.092 8.49E-8 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data