HIPHOP chemogenomics database

YKR035C_d / OPI8

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR035C / OPI8

Click on Significant Values for Screen Details

Top fitness defect scores for YKR035C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2619	obliquin	12.90	87.9 µM	4.10E-29	cell wall	11	1.41E-38
2	SGTC_1844	rapamycin	11.50	4.0 nM	1.59E-23		28	4.51E-31
3	SGTC_1593	justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one	9.88	54.9 µM	9.31E-18	redox potentiating	13	2.51E-23
4	SGTC_2679	rapamycin	7.62	1.0 nM	2.86E-11		46	1.27E-14
5	SGTC_145	3737-0113	7.14	38.4 µM	4.11E-10	DNA intercalators	14	4.52E-13
6	SGTC_3077	9117319	6.52	49.5 µM	1.07E-8		3	3.57E-11
7	SGTC_1926	methyl fluorone black	6.07	53.6 µM	9.28E-8	DNA intercalators	20	6.42E-10
8	SGTC_1750	st081859	5.87	2.4 µM	2.35E-7	endomembrane recycling	111	2.22E-9
9	SGTC_2582	agaric acid	5.68	100.0 µM	5.39E-7	redox potentiating	179	6.75E-9
10	SGTC_514	4466-0038	5.42	2.6 nM	1.65E-6	cell wall	93	3.01E-8
11	SGTC_619	1652-1634	5.28	108.0 µM	2.87E-6	PDR1	5	6.32E-8
12	SGTC_2137	5261020	4.92	140.0 µM	1.19E-5		22	4.22E-7
13	SGTC_1283	0922-0125	4.88	16.4 µM	1.40E-5		7	5.24E-7
14	SGTC_1583	clindamycin	4.86	39.6 µM	1.53E-5	mitochondrial processes	4	5.90E-7
15	SGTC_525	k781-2675	4.78	26.7 µM	2.09E-5	redox potentiating	35	8.94E-7
16	SGTC_2276	7947513	4.62	42.1 µM	3.76E-5		14	1.96E-6
17	SGTC_1269	0831-0796	4.60	3.0 µM	3.93E-5	60S ribosome export	6	2.07E-6
18	SGTC_2456	5770359	4.45	74.5 µM	6.88E-5		11	4.37E-6
19	SGTC_185	k048-0037	4.41	25.5 µM	7.85E-5	RSC & ERG11	5	5.21E-6
20	SGTC_1529	apigenin	4.24	74.0 µM	1.41E-4		29	1.14E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.316	1.58E-78	YPR173C	VPS4	hom	AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.312	2.02E-76	YLR119W	SRN2	hom	Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.245	7.73E-47	YKR035W-A	DID2	hom	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.242	7.23E-46	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.233	1.80E-42	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.221	1.93E-38	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.221	2.67E-38	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.218	2.46E-37	YPL084W	BRO1	hom	Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.214	5.35E-36	YGL212W	VAM7	hom	Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.213	1.11E-35	YNR006W	VPS27	hom	Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.211	4.16E-35	YKL002W	DID4	hom	Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.209	1.58E-34	YPL002C	SNF8	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.208	3.47E-34	YPL065W	VPS28	hom	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.207	7.20E-34	YMR077C	VPS20	hom	Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.206	2.05E-33	YDR049W	VMS1	hom	Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans