HIPHOP chemogenomics database

YKR043C / SHB17

Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR043C / SHB17

Click on Significant Values for Screen Details

Top fitness defect scores for YKR043C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1206	3978-0017	6.94	132.0 µM	3.18E-10	ERG2	8	1.96E-12
2	SGTC_2682	pyrogallin	6.13	40.0 µM	2.51E-8	copper-dependent oxidative stress	3	4.45E-10
3	SGTC_6	0844-0013	6.08	19.8 µM	3.13E-8		14	5.87E-10
4	SGTC_876	0720-0963	5.66	56.8 µM	2.46E-7		4	7.60E-9
5	SGTC_2940	9060638	5.19	45.9 µM	2.00E-6	amide catabolism	6	1.03E-7
6	SGTC_78	0336-0123	5.17	156.8 µM	2.17E-6		10	1.14E-7
7	SGTC_1937	st074714	4.99	47.2 µM	4.69E-6		20	2.97E-7
8	SGTC_3317	9138495	4.93	8.8 µM	6.17E-6		20	4.17E-7
9	SGTC_315	k915-0449	4.70	187.0 µM	1.52E-5		40	1.27E-6
10	SGTC_2966	9084041	4.46	48.0 µM	3.91E-5		21	4.12E-6
11	SGTC_3250	9135583	4.45	49.5 µM	4.03E-5		6	4.29E-6
12	SGTC_2842	9000675	4.41	19.5 µM	4.68E-5		13	5.16E-6
13	SGTC_3029	9088683	4.32	49.5 µM	6.51E-5		20	7.78E-6
14	SGTC_411	enrofloxacin	4.28	100.0 µM	7.61E-5	DNA damage response	7	9.44E-6
15	SGTC_2425	5628681	4.22	7.8 µM	9.33E-5		16	1.22E-5
16	SGTC_1245	0418-0076	4.19	17.2 µM	1.05E-4	ERG2	25	1.41E-5
17	SGTC_409	arachidonic acid	4.16	100.0 µM	1.14E-4		17	1.56E-5
18	SGTC_3052	9092717	4.10	49.5 µM	1.41E-4		23	2.03E-5
19	SGTC_1190	1486-1332	4.02	13.0 µM	1.90E-4		11	2.94E-5
20	SGTC_919	tnp00227	3.91	403.6 nM	2.69E-4	RNA pol III & RNase P/MRP	36	4.53E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.123	7.95E-13	YNL149C	PGA2	het	Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
0.115	2.56E-11	YLR345W_p	YLR345W_p	hom	Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
0.110	1.58E-10	YEL023C_p	YEL023C_p	hom	Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
0.103	2.46E-9	YML088W	UFO1	hom	F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
0.094	4.31E-8	YKL146W	AVT3	hom	Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.093	7.12E-8	YBR025C	OLA1	hom	P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
0.091	1.40E-7	YDL029W	ARP2	het	Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.088	3.14E-7	YIL136W	OM45	hom	Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress
0.087	4.81E-7	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.085	8.32E-7	YDR051C	DET1	hom	Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.085	8.53E-7	YLR398C	SKI2	hom	Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.084	9.81E-7	YOR027W	STI1	hom	Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
0.082	1.95E-6	YDL048C	STP4	hom	Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress
0.080	3.09E-6	YBR089C-A	NHP6B	hom	High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
0.080	3.86E-6	YNL150W_d	YNL150W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking