YKR045C_p

Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR045C / YKR045C

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:3.16|P-value:7.96E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.46|P-value:2.35E-8 ID:SGTC_296|Compound:1222-0039|FD-Score:3.39|P-value:3.46E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.24|P-value:5.89E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.49|P-value:2.46E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.95|P-value:3.64E-7 ID:SGTC_612|Compound:0302-0167|FD-Score:3.43|P-value:3.05E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.44|P-value:2.94E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.27|P-value:5.37E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.49|P-value:3.56E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:3.09|P-value:9.99E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:4.68|P-value:1.40E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.22|P-value:6.24E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:4.38|P-value:5.88E-6 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.43|P-value:3.06E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.17|P-value:7.55E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.72|P-value:5.08E-9 ID:SGTC_1193|Compound:1488-0126|FD-Score:6.53|P-value:3.23E-11 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.34|P-value:4.05E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.66|P-value:1.61E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.19|P-value:7.09E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.23|P-value:6.28E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.67|P-value:1.23E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.16|P-value:7.95E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.08|P-value:2.25E-5 ID:SGTC_1912|Compound:st060209|FD-Score:-3.26|P-value:5.45E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.86|P-value:5.70E-5 ID:SGTC_1940|Compound:st075990|FD-Score:-4.30|P-value:8.21E-6 ID:SGTC_1944|Compound:st076371|FD-Score:-3.22|P-value:6.39E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.57|P-value:1.78E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-3.36|P-value:3.76E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.37|P-value:3.74E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.27|P-value:5.46E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.22|P-value:1.21E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.27|P-value:5.47E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.19|P-value:7.02E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.40|P-value:3.41E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.58|P-value:1.67E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.89|P-value:5.00E-5 ID:SGTC_3137|Compound:9097352|FD-Score:3.15|P-value:8.29E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.32|P-value:4.38E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.20|P-value:6.79E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.24|P-value:5.91E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.16|P-value:7.96E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.46|P-value:2.35E-8 ID:SGTC_296|Compound:1222-0039|FD-Score:3.39|P-value:3.46E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.24|P-value:5.89E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.49|P-value:2.46E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.95|P-value:3.64E-7 ID:SGTC_612|Compound:0302-0167|FD-Score:3.43|P-value:3.05E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.44|P-value:2.94E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.27|P-value:5.37E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.49|P-value:3.56E-6 ID:SGTC_879|Compound:0831-1030|FD-Score:3.09|P-value:9.99E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:4.68|P-value:1.40E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.22|P-value:6.24E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:4.38|P-value:5.88E-6 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.43|P-value:3.06E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.17|P-value:7.55E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:-5.72|P-value:5.08E-9 ID:SGTC_1193|Compound:1488-0126|FD-Score:6.53|P-value:3.23E-11 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.34|P-value:4.05E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.66|P-value:1.61E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.19|P-value:7.09E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.23|P-value:6.28E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.67|P-value:1.23E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.16|P-value:7.95E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.08|P-value:2.25E-5 ID:SGTC_1912|Compound:st060209|FD-Score:-3.26|P-value:5.45E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.86|P-value:5.70E-5 ID:SGTC_1940|Compound:st075990|FD-Score:-4.30|P-value:8.21E-6 ID:SGTC_1944|Compound:st076371|FD-Score:-3.22|P-value:6.39E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.57|P-value:1.78E-4 ID:SGTC_2050|Compound:5263056|FD-Score:-3.36|P-value:3.76E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.37|P-value:3.74E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.27|P-value:5.46E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.22|P-value:1.21E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.27|P-value:5.47E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.19|P-value:7.02E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.40|P-value:3.41E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.58|P-value:1.67E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.89|P-value:5.00E-5 ID:SGTC_3137|Compound:9097352|FD-Score:3.15|P-value:8.29E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.32|P-value:4.38E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.20|P-value:6.79E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.24|P-value:5.91E-4

Top fitness defect scores for YKR045C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1193 1488-0126 6.53 41.5 µM 3.23E-11 5 3.28E-11
2 SGTC_453 enantio-paf c16 4.95 10.0 µM 3.64E-7 89 3.66E-7
3 SGTC_932 2857-0803 4.68 154.0 µM 1.40E-6 20 1.40E-6
4 SGTC_1293 1000-1278 4.66 195.0 µM 1.61E-6 3 1.62E-6
5 SGTC_781 4335-2806 4.49 94.4 µM 3.56E-6 11 3.57E-6
6 SGTC_1075 aripiprazole 4.38 15.2 µM 5.88E-6 16 5.90E-6
7 SGTC_2426 5582046 4.22 32.2 µM 1.21E-5 21 1.21E-5
8 SGTC_1911 st060208 4.08 74.3 µM 2.25E-5 32 2.25E-5
9 SGTC_3077 9117319 3.89 49.5 µM 5.00E-5 20 5.00E-5
10 SGTC_1921 5740136 3.86 50.0 µM 5.70E-5 TRP & mitochondrial translation 64 5.71E-5
11 SGTC_1731 st035964 3.67 63.0 µM 1.23E-4 40 1.23E-4
12 SGTC_1957 st077035 3.57 62.4 µM 1.78E-4 24 1.77E-4
13 SGTC_450 aminopyrene 3.49 156.0 nM 2.46E-4 54 2.46E-4
14 SGTC_705 1124-0212 3.44 310.0 µM 2.94E-4 30 2.93E-4
15 SGTC_612 0302-0167 3.43 22.4 µM 3.05E-4 copper-dependent oxidative stress 60 3.05E-4
16 SGTC_1077 5-fluorocytosine 3.43 377.0 nM 3.06E-4 exosome 32 3.06E-4
17 SGTC_2479 5617353 3.40 43.9 µM 3.41E-4 63 3.40E-4
18 SGTC_296 1222-0039 3.39 74.9 µM 3.46E-4 25 3.45E-4
19 SGTC_2282 7944268 3.37 200.0 µM 3.74E-4 RPP1 & pyrimidine depletion 58 3.73E-4
20 SGTC_737 1950-0117 3.27 57.9 µM 5.37E-4 44 5.36E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.83E-18 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.118 6.30E-12 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.113 6.63E-11 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.113 6.67E-11 YJL130C URA2 hom Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
0.110 1.62E-10 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.102 2.84E-9 YNL313C EMW1 het Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
0.102 3.41E-9 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.100 6.55E-9 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.095 3.34E-8 YLR249W YEF3 het Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
0.094 5.44E-8 YOR366W_d YOR366W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
0.090 1.64E-7 YOL097C WRS1 het Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
0.086 6.39E-7 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.083 1.61E-6 YGL158W RCK1 hom Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
0.083 1.69E-6 YIR038C GTT1 hom ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
0.082 1.80E-6 YMR216C SKY1 hom SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1