YKR063C / LAS1

Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR063C / LAS1

Click on Significant Values for Screen Details ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.42|P-value:1.56E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:5.27|P-value:1.70E-8 ID:SGTC_269|Compound:exalamide|FD-Score:3.18|P-value:4.29E-4 ID:SGTC_277|Compound:nystatin|FD-Score:4.54|P-value:9.76E-7 ID:SGTC_327|Compound:1273-0057|FD-Score:3.01|P-value:8.10E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:4.47|P-value:1.40E-6 ID:SGTC_692|Compound:k241-0027|FD-Score:4.16|P-value:6.50E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.49|P-value:1.19E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-3.82|P-value:2.86E-5 ID:SGTC_747|Compound:3448-8087|FD-Score:-4.52|P-value:9.96E-7 ID:SGTC_817|Compound:1391-0715|FD-Score:2.96|P-value:9.86E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.21|P-value:3.62E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.74|P-value:4.57E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.51|P-value:1.03E-6 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.08|P-value:5.81E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.66|P-value:6.27E-5 ID:SGTC_1829|Compound:berberine|FD-Score:4.30|P-value:3.25E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.33|P-value:2.27E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.75|P-value:3.86E-5 ID:SGTC_2088|Compound:5278280|FD-Score:-3.25|P-value:3.13E-4 ID:SGTC_2094|Compound:5332512|FD-Score:3.29|P-value:2.90E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-2.98|P-value:8.45E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-3.64|P-value:6.21E-5 ID:SGTC_2113|Compound:5491891|FD-Score:3.40|P-value:1.88E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.07|P-value:6.49E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.38|P-value:2.02E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.05|P-value:6.61E-4 ID:SGTC_2341|Compound:7987486|FD-Score:5.28|P-value:1.53E-8 ID:SGTC_2342|Compound:7990637|FD-Score:-5.12|P-value:3.66E-8 ID:SGTC_2343|Compound:7990716|FD-Score:-5.32|P-value:1.06E-8 ID:SGTC_2348|Compound:9022543|FD-Score:3.67|P-value:6.17E-5 ID:SGTC_2393|Compound:9031399|FD-Score:-3.53|P-value:1.02E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-6.12|P-value:6.06E-11 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.33|P-value:2.50E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.68|P-value:5.76E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.92|P-value:2.02E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.81|P-value:3.27E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:4.80|P-value:2.39E-7 ID:SGTC_2801|Compound:7907800|FD-Score:-4.15|P-value:6.15E-6 ID:SGTC_2862|Compound:9027634|FD-Score:-5.38|P-value:7.57E-9 ID:SGTC_2871|Compound:9038510|FD-Score:2.99|P-value:8.75E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.61|P-value:6.13E-7 ID:SGTC_2947|Compound:9072639|FD-Score:3.18|P-value:4.30E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.00|P-value:8.59E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.06|P-value:6.91E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.12|P-value:5.41E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.03|P-value:7.54E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.10|P-value:5.91E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.10|P-value:5.51E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-4.49|P-value:1.14E-6 ID:SGTC_3212|Compound:9128307|FD-Score:-3.16|P-value:4.38E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.42|P-value:1.56E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:5.27|P-value:1.70E-8 ID:SGTC_269|Compound:exalamide|FD-Score:3.18|P-value:4.29E-4 ID:SGTC_277|Compound:nystatin|FD-Score:4.54|P-value:9.76E-7 ID:SGTC_327|Compound:1273-0057|FD-Score:3.01|P-value:8.10E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:4.47|P-value:1.40E-6 ID:SGTC_692|Compound:k241-0027|FD-Score:4.16|P-value:6.50E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.49|P-value:1.19E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:-3.82|P-value:2.86E-5 ID:SGTC_747|Compound:3448-8087|FD-Score:-4.52|P-value:9.96E-7 ID:SGTC_817|Compound:1391-0715|FD-Score:2.96|P-value:9.86E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.21|P-value:3.62E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.74|P-value:4.57E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.51|P-value:1.03E-6 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.08|P-value:5.81E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.66|P-value:6.27E-5 ID:SGTC_1829|Compound:berberine|FD-Score:4.30|P-value:3.25E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.33|P-value:2.27E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.75|P-value:3.86E-5 ID:SGTC_2088|Compound:5278280|FD-Score:-3.25|P-value:3.13E-4 ID:SGTC_2094|Compound:5332512|FD-Score:3.29|P-value:2.90E-4 ID:SGTC_2097|Compound:5376186|FD-Score:-2.98|P-value:8.45E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-3.64|P-value:6.21E-5 ID:SGTC_2113|Compound:5491891|FD-Score:3.40|P-value:1.88E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.07|P-value:6.49E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.38|P-value:2.02E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-3.05|P-value:6.61E-4 ID:SGTC_2341|Compound:7987486|FD-Score:5.28|P-value:1.53E-8 ID:SGTC_2342|Compound:7990637|FD-Score:-5.12|P-value:3.66E-8 ID:SGTC_2343|Compound:7990716|FD-Score:-5.32|P-value:1.06E-8 ID:SGTC_2348|Compound:9022543|FD-Score:3.67|P-value:6.17E-5 ID:SGTC_2393|Compound:9031399|FD-Score:-3.53|P-value:1.02E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-6.12|P-value:6.06E-11 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.33|P-value:2.50E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.68|P-value:5.76E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:3.92|P-value:2.02E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.81|P-value:3.27E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:4.80|P-value:2.39E-7 ID:SGTC_2801|Compound:7907800|FD-Score:-4.15|P-value:6.15E-6 ID:SGTC_2862|Compound:9027634|FD-Score:-5.38|P-value:7.57E-9 ID:SGTC_2871|Compound:9038510|FD-Score:2.99|P-value:8.75E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.61|P-value:6.13E-7 ID:SGTC_2947|Compound:9072639|FD-Score:3.18|P-value:4.30E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.00|P-value:8.59E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.06|P-value:6.91E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.12|P-value:5.41E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.03|P-value:7.54E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.10|P-value:5.91E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.10|P-value:5.51E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-4.49|P-value:1.14E-6 ID:SGTC_3212|Compound:9128307|FD-Score:-3.16|P-value:4.38E-4

Top fitness defect scores for YKR063C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2341 7987486 5.28 176.7 µM 1.53E-8 1 6.34E-8 0.72
2 SGTC_259 usnic acid 5.27 108.9 µM 1.70E-8 4 6.98E-8 0.93
3 SGTC_2659 sanguinarine 4.80 24.2 µM 2.39E-7 6 7.74E-7 0.37
4 SGTC_277 nystatin 4.54 15.5 µM 9.76E-7 1 2.79E-6 0.47
5 SGTC_504 lovastatin 4.47 124.0 µM 1.40E-6 3 3.89E-6 0.23
6 SGTC_1829 berberine 4.30 14.4 µM 3.25E-6 60S ribosome export 11 8.36E-6 0.17
7 SGTC_692 k241-0027 4.16 77.8 µM 6.50E-6 4 1.58E-5 0.23
8 SGTC_2600 tangeritin 3.92 88.0 µM 2.02E-5 5 4.44E-5 0.44
9 SGTC_2630 antimycin a 3.81 100.0 µM 3.27E-5 mitochondrial processes 12 6.89E-5 0.03
10 SGTC_1216 1165-0504 3.74 9.7 µM 4.57E-5 6 9.35E-5 0.05
11 SGTC_2552 juarezic acid 3.68 87.0 µM 5.76E-5 TSC3-RPN4 4 1.15E-4 0.43
12 SGTC_2348 9022543 3.67 33.4 µM 6.17E-5 mitochondrial processes 35 1.23E-4 0.03
13 SGTC_1611 st001856 3.66 99.4 µM 6.27E-5 11 1.25E-4 0.03
14 SGTC_2113 5491891 3.40 107.0 µM 1.88E-4 TSC3-RPN4 10 3.41E-4 0.20
15 SGTC_2162 5663267 3.38 200.0 µM 2.02E-4 16 3.64E-4 0.04
16 SGTC_2408 sulbentine 3.33 18.4 µM 2.50E-4 49 4.42E-4 0.04
17 SGTC_2094 5332512 3.29 191.7 µM 2.90E-4 9 5.07E-4 0.04
18 SGTC_269 exalamide 3.18 29.0 µM 4.29E-4 ergosterol biosynthesis 13 7.25E-4 0.30
19 SGTC_2947 9072639 3.18 7.7 µM 4.30E-4 tubulin folding & SWR complex 18 7.27E-4 0.17
20 SGTC_3082 9117008 3.12 49.5 µM 5.41E-4 4 8.97E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 7.88E-14 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.117 9.26E-12 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.110 1.94E-10 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.102 3.17E-9 YBR264C YPT10 hom Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
0.097 1.62E-8 YDR498C SEC20 het Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
0.094 5.19E-8 YER038C KRE29 het Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.093 6.40E-8 YDL027C_p YDL027C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.093 7.35E-8 YJL050W MTR4 het ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.091 1.13E-7 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.091 1.35E-7 YDR365C ESF1 het Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
0.088 3.30E-7 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.086 6.17E-7 YDR037W KRS1 het Lysyl-tRNA synthetase
0.086 6.60E-7 YLR265C NEJ1 hom Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p
0.084 1.05E-6 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.084 1.16E-6 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)