HIPHOP chemogenomics database

YKR064W / OAF3

Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR064W / OAF3

Click on Significant Values for Screen Details

Top fitness defect scores for YKR064W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_70	0141-0289	7.59	43.3 µM	1.94E-13	mitochondrial stress	45	1.60E-14
2	SGTC_145	3737-0113	6.34	38.4 µM	6.82E-10	DNA intercalators	24	1.16E-10
3	SGTC_78	0336-0123	5.47	156.8 µM	8.79E-8		8	2.31E-8
4	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	4.49	3.7 µM	8.90E-6		50	3.52E-6
5	SGTC_2975	9084847	4.49	53.7 µM	9.17E-6		15	3.63E-6
6	SGTC_144	4161-2749	4.05	112.4 µM	5.46E-5	DNA intercalators	23	2.53E-5
7	SGTC_9	0092-0002	3.90	10.4 µM	9.67E-5		28	4.72E-5
8	SGTC_656	4090-1979	3.86	187.0 µM	1.15E-4		21	5.68E-5
9	SGTC_3335	9143315	3.79	29.6 µM	1.51E-4	fatty acid desaturase (OLE1)	15	7.65E-5
10	SGTC_184	1494-0393	3.70	24.7 µM	2.05E-4		70	1.07E-4
11	SGTC_649	1123-0311	3.69	367.0 µM	2.15E-4	endomembrane recycling	81	1.12E-4
12	SGTC_2596	sinensetin	3.65	100.0 µM	2.49E-4		42	1.32E-4
13	SGTC_1821	st053465	3.54	65.6 µM	3.68E-4		14	2.02E-4
14	SGTC_6	0844-0013	3.49	19.8 µM	4.37E-4		141	2.43E-4
15	SGTC_1241	0364-0022	3.38	50.8 µM	6.33E-4	ERG2	50	3.64E-4
16	SGTC_2962	9082311	3.34	13.2 µM	7.09E-4		41	4.12E-4
17	SGTC_619	1652-1634	3.27	108.0 µM	9.20E-4	PDR1	44	5.46E-4
18	SGTC_324	1315-0088	3.25	19.6 µM	9.64E-4	RSC & ERG11	22	5.75E-4
19	SGTC_2762	leflunomide	3.22	42.1 µM	0.00107		59	6.41E-4
20	SGTC_1982	st072467	3.21	71.9 µM	0.00111		73	6.69E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.204	1.11E-32	YDR401W_d	YDR401W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.198	8.74E-31	YEL017C-A	PMP2	hom	Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.175	1.86E-24	YGL002W	ERP6	hom	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.166	3.46E-22	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.161	8.16E-21	YFL022C	FRS2	het	Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.154	2.98E-19	YMR030W	RSF1	hom	Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.153	7.10E-19	YOR077W	RTS2	het	Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.150	3.42E-18	YNL124W	NAF1	het	RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.145	2.88E-17	YBL029W_p	YBL029W_p	hom	Non-essential protein of unknown function
0.144	7.28E-17	YKL205W	LOS1	hom	Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.142	1.26E-16	YBR182C-A_p	YBR182C-A_p	hom	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.142	1.76E-16	YPR059C_d	YPR059C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.141	3.15E-16	YDR414C	ERD1	hom	Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.139	5.98E-16	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.138	9.24E-16	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain