HIPHOP chemogenomics database

YKR069W / MET1

S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR069W / MET1

Click on Significant Values for Screen Details

Top fitness defect scores for YKR069W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_6	0844-0013	6.23	19.8 µM	2.37E-10		12	2.37E-10
2	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	5.16	3.7 µM	1.23E-7		28	1.23E-7
3	SGTC_1049	3226-0134	4.10	66.0 µM	2.05E-5		23	2.04E-5
4	SGTC_2604	4'-hydroxychalcone	4.07	11.3 µM	2.40E-5		32	2.39E-5
5	SGTC_2964	9083345	3.96	53.4 µM	3.70E-5		19	3.69E-5
6	SGTC_3078	9116413	3.94	49.5 µM	4.14E-5	unfolded protein response	36	4.12E-5
7	SGTC_2624	nerol	3.71	100.0 µM	1.04E-4		12	1.04E-4
8	SGTC_2855	9016525	3.60	58.4 µM	1.63E-4		27	1.62E-4
9	SGTC_806	3966-4455	3.57	5.9 µM	1.77E-4		32	1.76E-4
10	SGTC_3286	9117135	3.50	37.2 µM	2.36E-4		29	2.35E-4
11	SGTC_1040	k898-0338	3.47	155.0 µM	2.64E-4		32	2.63E-4
12	SGTC_2699	st077704	3.39	54.6 µM	3.56E-4	endomembrane recycling	72	3.54E-4
13	SGTC_1860	st056228	3.36	74.6 µM	3.96E-4		37	3.94E-4
14	SGTC_1155	4088-0394	3.33	48.2 µM	4.33E-4		54	4.30E-4
15	SGTC_3267	9137951	3.32	49.5 µM	4.51E-4		17	4.48E-4
16	SGTC_3298	9116858	3.32	71.4 µM	4.59E-4		29	4.57E-4
17	SGTC_2145	5634607	3.31	200.0 µM	4.75E-4		20	4.73E-4
18	SGTC_1743	st037606	3.30	46.4 µM	4.92E-4		44	4.89E-4
19	SGTC_160	3453-0671	3.29	289.7 µM	4.98E-4		67	4.96E-4
20	SGTC_1028	k015-0029	3.27	4.8 µM	5.40E-4		47	5.37E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.184	4.59E-27	YOR155C	ISN1	hom	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.176	9.78E-25	YMR145C	NDE1	hom	Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain
0.155	1.83E-19	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.152	9.98E-19	YDR085C	AFR1	hom	Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.150	1.97E-18	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.141	2.68E-16	YNL242W	ATG2	hom	Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.139	4.93E-16	YNL218W	MGS1	hom	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.139	5.06E-16	YKR009C	FOX2	hom	Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.139	5.24E-16	YLR288C	MEC3	hom	DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.137	1.30E-15	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.135	3.33E-15	YOR113W	AZF1	hom	Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
0.129	5.43E-14	YBL028C	YBL028C	hom	Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.129	6.10E-14	YOR214C_p	YOR214C_p	hom	Putative protein of unknown function; YOR214C is not an essential gene
0.129	7.45E-14	YFL046W_p	FMP32_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.127	1.88E-13	YGL017W	ATE1	hom	Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway