YKR069W / MET1

S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR069W / MET1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:6.23|P-value:2.37E-10 ID:SGTC_160|Compound:3453-0671|FD-Score:3.29|P-value:4.98E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:-3.74|P-value:9.00E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.57|P-value:1.77E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.27|P-value:5.47E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.27|P-value:5.40E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.47|P-value:2.64E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.10|P-value:2.05E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.33|P-value:4.33E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.19|P-value:7.24E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.30|P-value:4.92E-4 ID:SGTC_1839|Compound:st053978|FD-Score:3.10|P-value:9.67E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.19|P-value:7.06E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.36|P-value:3.96E-4 ID:SGTC_2139|Compound:5578692|FD-Score:-3.37|P-value:3.69E-4 ID:SGTC_2145|Compound:5634607|FD-Score:3.31|P-value:4.75E-4 ID:SGTC_2151|Compound:5563267|FD-Score:-3.19|P-value:6.96E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.16|P-value:1.23E-7 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.16|P-value:7.80E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.07|P-value:2.40E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.71|P-value:1.21E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.10|P-value:9.89E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.71|P-value:1.04E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.39|P-value:3.56E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.60|P-value:1.63E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.96|P-value:3.70E-5 ID:SGTC_2998|Compound:9066762|FD-Score:-3.19|P-value:7.02E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.94|P-value:4.14E-5 ID:SGTC_3200|Compound:9114385|FD-Score:-3.49|P-value:2.38E-4 ID:SGTC_3267|Compound:9137951|FD-Score:3.32|P-value:4.51E-4 ID:SGTC_3279|Compound:9140807|FD-Score:-3.75|P-value:8.79E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.50|P-value:2.36E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.32|P-value:4.59E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:6.23|P-value:2.37E-10 ID:SGTC_160|Compound:3453-0671|FD-Score:3.29|P-value:4.98E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:-3.74|P-value:9.00E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.57|P-value:1.77E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.27|P-value:5.47E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.27|P-value:5.40E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.47|P-value:2.64E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.10|P-value:2.05E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.33|P-value:4.33E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.19|P-value:7.24E-4 ID:SGTC_1743|Compound:st037606|FD-Score:3.30|P-value:4.92E-4 ID:SGTC_1839|Compound:st053978|FD-Score:3.10|P-value:9.67E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.19|P-value:7.06E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.36|P-value:3.96E-4 ID:SGTC_2139|Compound:5578692|FD-Score:-3.37|P-value:3.69E-4 ID:SGTC_2145|Compound:5634607|FD-Score:3.31|P-value:4.75E-4 ID:SGTC_2151|Compound:5563267|FD-Score:-3.19|P-value:6.96E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.18|P-value:7.36E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.16|P-value:1.23E-7 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.16|P-value:7.80E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.07|P-value:2.40E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:-4.71|P-value:1.21E-6 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.10|P-value:9.89E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.71|P-value:1.04E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.39|P-value:3.56E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.60|P-value:1.63E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.96|P-value:3.70E-5 ID:SGTC_2998|Compound:9066762|FD-Score:-3.19|P-value:7.02E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.94|P-value:4.14E-5 ID:SGTC_3200|Compound:9114385|FD-Score:-3.49|P-value:2.38E-4 ID:SGTC_3267|Compound:9137951|FD-Score:3.32|P-value:4.51E-4 ID:SGTC_3279|Compound:9140807|FD-Score:-3.75|P-value:8.79E-5 ID:SGTC_3286|Compound:9117135|FD-Score:3.50|P-value:2.36E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.32|P-value:4.59E-4

Top fitness defect scores for YKR069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 6.23 19.8 µM 2.37E-10 12 2.37E-10
2 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 5.16 3.7 µM 1.23E-7 28 1.23E-7
3 SGTC_1049 3226-0134 4.10 66.0 µM 2.05E-5 23 2.04E-5
4 SGTC_2604 4'-hydroxychalcone 4.07 11.3 µM 2.40E-5 32 2.39E-5
5 SGTC_2964 9083345 3.96 53.4 µM 3.70E-5 19 3.69E-5
6 SGTC_3078 9116413 3.94 49.5 µM 4.14E-5 unfolded protein response 36 4.12E-5
7 SGTC_2624 nerol 3.71 100.0 µM 1.04E-4 12 1.04E-4
8 SGTC_2855 9016525 3.60 58.4 µM 1.63E-4 27 1.62E-4
9 SGTC_806 3966-4455 3.57 5.9 µM 1.77E-4 32 1.76E-4
10 SGTC_3286 9117135 3.50 37.2 µM 2.36E-4 29 2.35E-4
11 SGTC_1040 k898-0338 3.47 155.0 µM 2.64E-4 32 2.63E-4
12 SGTC_2699 st077704 3.39 54.6 µM 3.56E-4 endomembrane recycling 72 3.54E-4
13 SGTC_1860 st056228 3.36 74.6 µM 3.96E-4 37 3.94E-4
14 SGTC_1155 4088-0394 3.33 48.2 µM 4.33E-4 54 4.30E-4
15 SGTC_3267 9137951 3.32 49.5 µM 4.51E-4 17 4.48E-4
16 SGTC_3298 9116858 3.32 71.4 µM 4.59E-4 29 4.57E-4
17 SGTC_2145 5634607 3.31 200.0 µM 4.75E-4 20 4.73E-4
18 SGTC_1743 st037606 3.30 46.4 µM 4.92E-4 44 4.89E-4
19 SGTC_160 3453-0671 3.29 289.7 µM 4.98E-4 67 4.96E-4
20 SGTC_1028 k015-0029 3.27 4.8 µM 5.40E-4 47 5.37E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 4.59E-27 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.176 9.78E-25 YMR145C NDE1 hom Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain
0.155 1.83E-19 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.152 9.98E-19 YDR085C AFR1 hom Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
0.150 1.97E-18 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.141 2.68E-16 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.139 4.93E-16 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.139 5.06E-16 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.139 5.24E-16 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.137 1.30E-15 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.135 3.33E-15 YOR113W AZF1 hom Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity
0.129 5.43E-14 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.129 6.10E-14 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.129 7.45E-14 YFL046W_p FMP32_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.127 1.88E-13 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway