YKR080W / MTD1

NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR080W / MTD1

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:6.49|P-value:1.85E-10 ID:SGTC_51|Compound:1013-0126|FD-Score:5.39|P-value:1.01E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:7.31|P-value:8.55E-13 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.22|P-value:9.74E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.55|P-value:3.09E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:3.27|P-value:8.15E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:6.81|P-value:2.37E-11 ID:SGTC_1250|Compound:0485-0349|FD-Score:7.62|P-value:9.26E-14 ID:SGTC_1605|Compound:st000082|FD-Score:3.47|P-value:4.14E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.33|P-value:6.12E-4 ID:SGTC_2135|Compound:5326629|FD-Score:4.48|P-value:7.89E-6 ID:SGTC_2207|Compound:7258803|FD-Score:-3.92|P-value:7.14E-5 ID:SGTC_2362|Compound:9071156|FD-Score:-3.82|P-value:1.04E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.67|P-value:1.82E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:4.55|P-value:5.78E-6 ID:SGTC_2657|Compound:nystatin|FD-Score:3.64|P-value:2.23E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.51|P-value:3.52E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.61|P-value:2.30E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.28|P-value:7.88E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.99|P-value:6.05E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.52|P-value:3.52E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-3.55|P-value:2.91E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.56|P-value:2.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.65|P-value:2.15E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.56|P-value:2.78E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:6.49|P-value:1.85E-10 ID:SGTC_51|Compound:1013-0126|FD-Score:5.39|P-value:1.01E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:7.31|P-value:8.55E-13 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.22|P-value:9.74E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:3.55|P-value:3.09E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:3.27|P-value:8.15E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:6.81|P-value:2.37E-11 ID:SGTC_1250|Compound:0485-0349|FD-Score:7.62|P-value:9.26E-14 ID:SGTC_1605|Compound:st000082|FD-Score:3.47|P-value:4.14E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.33|P-value:6.12E-4 ID:SGTC_2135|Compound:5326629|FD-Score:4.48|P-value:7.89E-6 ID:SGTC_2207|Compound:7258803|FD-Score:-3.92|P-value:7.14E-5 ID:SGTC_2362|Compound:9071156|FD-Score:-3.82|P-value:1.04E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.67|P-value:1.82E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:4.55|P-value:5.78E-6 ID:SGTC_2657|Compound:nystatin|FD-Score:3.64|P-value:2.23E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.51|P-value:3.52E-4 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-3.61|P-value:2.30E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.28|P-value:7.88E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.99|P-value:6.05E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.52|P-value:3.52E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-3.55|P-value:2.91E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.56|P-value:2.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.65|P-value:2.15E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.56|P-value:2.78E-4

Top fitness defect scores for YKR080W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1250 0485-0349 7.62 40.2 µM 9.26E-14 2 1.26E-14
2 SGTC_123 0083-0120 7.31 66.7 µM 8.55E-13 11 1.36E-13
3 SGTC_1190 1486-1332 6.81 13.0 µM 2.37E-11 1 4.75E-12
4 SGTC_47 0149-0135 6.49 24.2 µM 1.85E-10 TSC3-RPN4 2 4.27E-11
5 SGTC_51 1013-0126 5.39 23.4 µM 1.01E-7 18 3.61E-8
6 SGTC_2635 hydroquinidine 4.55 100.0 µM 5.78E-6 12 2.73E-6
7 SGTC_2135 5326629 4.48 150.4 µM 7.89E-6 RPP1 & pyrimidine depletion 15 3.81E-6
8 SGTC_2975 9084847 3.99 53.7 µM 6.05E-5 21 3.36E-5
9 SGTC_3131 9127911 3.65 49.5 µM 2.15E-4 40 1.30E-4
10 SGTC_2657 nystatin 3.64 74.4 µM 2.23E-4 20 1.35E-4
11 SGTC_781 4335-2806 3.55 94.4 µM 3.09E-4 31 1.92E-4
12 SGTC_2977 9011197 3.52 71.4 µM 3.52E-4 29 2.20E-4
13 SGTC_2663 amphotericin b 3.51 100.0 µM 3.52E-4 20 2.20E-4
14 SGTC_1605 st000082 3.47 49.1 µM 4.14E-4 60S ribosome export 31 2.62E-4
15 SGTC_2833 9001315 3.28 45.5 µM 7.88E-4 amide catabolism 86 5.20E-4
16 SGTC_818 4072-2707 3.27 53.5 µM 8.15E-4 33 5.40E-4
17 SGTC_246 cycloheximide 3.22 667.0 µM 9.74E-4 34 6.52E-4
18 SGTC_2194 5927483 3.18 104.5 µM 0.00109 44 7.34E-4
19 SGTC_2070 5230947 3.17 200.0 µM 0.00112 40 7.59E-4
20 SGTC_3350 9150499 3.17 3.6 µM 0.00114 cell wall 91 7.73E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.230 2.13E-41 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.138 1.14E-15 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.136 2.35E-15 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.130 4.01E-14 YMR075W RCO1 hom Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
0.127 1.35E-13 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.120 2.75E-12 YGR024C THG1 het tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
0.120 3.53E-12 YKL125W RRN3 het Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
0.107 5.85E-10 YGR061C ADE6 hom Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.094 4.99E-8 YMR120C ADE17 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.092 8.76E-8 YGL032C AGA2 hom Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
0.090 2.02E-7 YPR118W MRI1 hom 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
0.089 2.43E-7 YBL071C_d YBL071C_d hom Dubious open reading frame, predicted protein contains a peroxisomal targeting signal
0.089 2.69E-7 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.088 3.02E-7 YNL212W VID27 hom Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
0.087 4.84E-7 YPR105C COG4 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments