YLL062C / MHT1

S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL062C / MHT1

Click on Significant Values for Screen Details ID:SGTC_366|Compound:0181-0206|FD-Score:3.33|P-value:3.01E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.64|P-value:8.18E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.01|P-value:8.98E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:4.37|P-value:3.30E-6 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.34|P-value:2.69E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.97|P-value:2.12E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.45|P-value:1.93E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.37|P-value:1.58E-8 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.51|P-value:1.41E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.71|P-value:6.18E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.06|P-value:8.26E-4 ID:SGTC_1741|Compound:st038134|FD-Score:4.07|P-value:1.35E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.59|P-value:1.09E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.19|P-value:5.03E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.68|P-value:7.57E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.70|P-value:6.99E-5 ID:SGTC_2717|Compound:st081029|FD-Score:-3.02|P-value:8.63E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.12|P-value:6.62E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-3.28|P-value:3.36E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.63|P-value:8.57E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.01|P-value:9.66E-4 ID:SGTC_3261|Compound:9137475|FD-Score:-3.02|P-value:8.75E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.77|P-value:5.23E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.14|P-value:6.19E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.33|P-value:3.01E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.64|P-value:8.18E-5 ID:SGTC_540|Compound:1187-0821|FD-Score:-3.01|P-value:8.98E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:4.37|P-value:3.30E-6 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.34|P-value:2.69E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.97|P-value:2.12E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.45|P-value:1.93E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.37|P-value:1.58E-8 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.51|P-value:1.41E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.71|P-value:6.18E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.06|P-value:8.26E-4 ID:SGTC_1741|Compound:st038134|FD-Score:4.07|P-value:1.35E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.59|P-value:1.09E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.19|P-value:5.03E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.68|P-value:7.57E-5 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.70|P-value:6.99E-5 ID:SGTC_2717|Compound:st081029|FD-Score:-3.02|P-value:8.63E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.12|P-value:6.62E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-3.28|P-value:3.36E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.63|P-value:8.57E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.01|P-value:9.66E-4 ID:SGTC_3261|Compound:9137475|FD-Score:-3.02|P-value:8.75E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.77|P-value:5.23E-5 ID:SGTC_3331|Compound:9141060|FD-Score:3.14|P-value:6.19E-4

Top fitness defect scores for YLL062C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1239 0337-0584 5.37 35.4 µM 1.58E-8 unfolded protein response 22 4.01E-8
2 SGTC_693 4161-2696 4.37 114.0 µM 3.30E-6 4 6.13E-6
3 SGTC_1741 st038134 4.07 10.2 µM 1.35E-5 28 2.31E-5
4 SGTC_1033 k072-0102 3.97 21.3 µM 2.12E-5 60S ribosome export 21 3.54E-5
5 SGTC_3329 9141233 3.77 27.6 µM 5.23E-5 18 8.28E-5
6 SGTC_2611 pyromycin 3.70 23.9 µM 6.99E-5 33 1.09E-4
7 SGTC_2606 farnesol 3.68 9.8 µM 7.57E-5 25 1.17E-4
8 SGTC_2419 paclitaxel 3.59 166.5 µM 1.09E-4 58 1.66E-4
9 SGTC_1044 1222-0021 3.45 1.8 µM 1.93E-4 heme biosynthesis & mitochondrial translocase 53 2.84E-4
10 SGTC_366 0181-0206 3.33 4.3 µM 3.01E-4 Golgi 71 4.32E-4
11 SGTC_2484 5809818 3.19 12.0 µM 5.03E-4 37 7.01E-4
12 SGTC_3331 9141060 3.14 2.0 µM 6.19E-4 cell wall 61 8.52E-4
13 SGTC_2857 9024691 3.12 58.4 µM 6.62E-4 61 9.09E-4
14 SGTC_1428 4049-0204 3.06 61.7 µM 8.26E-4 45 0.00112
15 SGTC_2974 9094440 3.01 9.7 µM 9.66E-4 ergosterol biosynthesis 96 0.00130
16 SGTC_2294 7971431 2.98 200.0 µM 0.00107 54 0.00143
17 SGTC_2762 leflunomide 2.98 42.1 µM 0.00109 78 0.00145
18 SGTC_1032 k061-1018 2.89 98.7 µM 0.00148 82 0.00195
19 SGTC_656 4090-1979 2.86 187.0 µM 0.00161 79 0.00210
20 SGTC_2159 5658611 2.72 73.8 µM 0.00255 43 0.00325

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 2.11E-42 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.207 6.80E-34 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.148 7.04E-18 YFL028C CAF16 hom Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
0.135 5.00E-15 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.128 8.62E-14 YGL156W AMS1 hom Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
0.128 9.62E-14 YLR132C USB1 het Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
0.125 3.20E-13 YJR152W DAL5 hom Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
0.122 1.17E-12 YBL028C YBL028C hom Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.121 2.10E-12 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.119 4.51E-12 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.118 7.62E-12 YJR061W_p YJR061W_p hom Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication
0.113 6.24E-11 YLL060C GTT2 hom Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
0.113 6.65E-11 YKL184W SPE1 hom Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
0.111 1.37E-10 YHL012W_p YHL012W_p hom Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
0.110 1.61E-10 YGL170C SPO74 hom Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation