YLR003C / CMS1

Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR003C / CMS1

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:2.91|P-value:6.50E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.54|P-value:4.51E-5 ID:SGTC_76|Compound:0433-0009|FD-Score:-3.05|P-value:4.37E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-2.83|P-value:9.98E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.15|P-value:2.91E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-2.91|P-value:7.41E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.33|P-value:8.62E-7 ID:SGTC_516|Compound:3992-2719|FD-Score:3.39|P-value:9.01E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.37|P-value:1.17E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.27|P-value:1.48E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.21|P-value:2.26E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:2.80|P-value:9.80E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-2.86|P-value:8.91E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.24|P-value:2.05E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.05|P-value:3.75E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.13|P-value:2.70E-4 ID:SGTC_1115|Compound:galangin|FD-Score:2.86|P-value:7.88E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-2.98|P-value:5.75E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.07|P-value:3.41E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.05|P-value:4.39E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:-2.93|P-value:6.97E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.95|P-value:6.46E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.21|P-value:1.92E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.29|P-value:1.37E-4 ID:SGTC_1620|Compound:st003704|FD-Score:-4.30|P-value:1.27E-6 ID:SGTC_1647|Compound:st011722|FD-Score:3.21|P-value:1.93E-4 ID:SGTC_1760|Compound:st045453|FD-Score:2.94|P-value:5.78E-4 ID:SGTC_1989|Compound:st071868|FD-Score:2.84|P-value:8.38E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.93|P-value:5.84E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.92|P-value:6.22E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.82|P-value:9.13E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-2.94|P-value:6.72E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.00|P-value:5.11E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.15|P-value:2.87E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:2.91|P-value:6.43E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.30|P-value:1.32E-4 ID:SGTC_2780|Compound:5215298|FD-Score:2.94|P-value:5.75E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.85|P-value:8.17E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-2.99|P-value:5.44E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.02|P-value:4.21E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:2.91|P-value:6.50E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.54|P-value:4.51E-5 ID:SGTC_76|Compound:0433-0009|FD-Score:-3.05|P-value:4.37E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-2.83|P-value:9.98E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.15|P-value:2.91E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-2.91|P-value:7.41E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:4.33|P-value:8.62E-7 ID:SGTC_516|Compound:3992-2719|FD-Score:3.39|P-value:9.01E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.37|P-value:1.17E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.27|P-value:1.48E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.21|P-value:2.26E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:2.80|P-value:9.80E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-2.86|P-value:8.91E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.24|P-value:2.05E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.05|P-value:3.75E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.13|P-value:2.70E-4 ID:SGTC_1115|Compound:galangin|FD-Score:2.86|P-value:7.88E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-2.98|P-value:5.75E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.07|P-value:3.41E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.05|P-value:4.39E-4 ID:SGTC_1328|Compound:1441-0025|FD-Score:-2.93|P-value:6.97E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.95|P-value:6.46E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.21|P-value:1.92E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.29|P-value:1.37E-4 ID:SGTC_1620|Compound:st003704|FD-Score:-4.30|P-value:1.27E-6 ID:SGTC_1647|Compound:st011722|FD-Score:3.21|P-value:1.93E-4 ID:SGTC_1760|Compound:st045453|FD-Score:2.94|P-value:5.78E-4 ID:SGTC_1989|Compound:st071868|FD-Score:2.84|P-value:8.38E-4 ID:SGTC_1997|Compound:st073024|FD-Score:2.93|P-value:5.84E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.92|P-value:6.22E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.82|P-value:9.13E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-2.94|P-value:6.72E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.00|P-value:5.11E-6 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.15|P-value:2.87E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:2.91|P-value:6.43E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.30|P-value:1.32E-4 ID:SGTC_2780|Compound:5215298|FD-Score:2.94|P-value:5.75E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.85|P-value:8.17E-4 ID:SGTC_2848|Compound:9016370|FD-Score:-2.99|P-value:5.44E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.02|P-value:4.21E-4

Top fitness defect scores for YLR003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_362 0310-0100 4.33 9.0 µM 8.62E-7 RPP1 & pyrimidine depletion 24 7.35E-6
2 SGTC_2150 5556613 4.00 200.0 µM 5.11E-6 20 3.23E-5
3 SGTC_1338 1486-1223 3.95 76.0 µM 6.46E-6 23 3.93E-5
4 SGTC_65 0798-0905 3.54 35.0 µM 4.51E-5 calcium & mitochondrial duress 111 1.98E-4
5 SGTC_516 3992-2719 3.39 87.3 µM 9.01E-5 30 3.52E-4
6 SGTC_2506 ethyl hematommate 3.30 54.2 µM 1.32E-4 RPP1 & pyrimidine depletion 38 4.84E-4
7 SGTC_1371 2421-0008 3.29 32.7 µM 1.37E-4 mitochondrial processes 71 4.99E-4
8 SGTC_631 k072-0230 3.27 41.5 µM 1.48E-4 60S ribosome export 43 5.31E-4
9 SGTC_1340 1486-1359 3.21 257.0 µM 1.92E-4 46 6.60E-4
10 SGTC_1647 st011722 3.21 22.4 µM 1.93E-4 27 6.64E-4
11 SGTC_1103 duloxetine 3.13 36.1 µM 2.70E-4 67 8.80E-4
12 SGTC_1205 4161-2785 3.07 76.0 µM 3.41E-4 68 0.00107
13 SGTC_1087 pterostilbene 3.05 9.1 µM 3.75E-4 75 0.00116
14 SGTC_3354 9151612 3.02 71.4 µM 4.21E-4 46 0.00127
15 SGTC_2780 5215298 2.94 71.4 µM 5.75E-4 97 0.00165
16 SGTC_1760 st045453 2.94 10.4 µM 5.78E-4 NEO1-PIK1 197 0.00166
17 SGTC_1997 st073024 2.93 93.8 µM 5.84E-4 38 0.00167
18 SGTC_2050 5263056 2.92 171.0 µM 6.22E-4 fatty acid desaturase (OLE1) 103 0.00176
19 SGTC_2504 purpurogallin 2.91 52.3 µM 6.43E-4 RPP1 & pyrimidine depletion 58 0.00181
20 SGTC_22 0964-0034 2.91 14.5 µM 6.50E-4 unfolded protein response 100 0.00183

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 7.47E-25 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.126 1.96E-13 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.125 3.63E-13 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.125 3.88E-13 YBR139W YBR139W hom Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
0.117 1.13E-11 YLR276C DBP9 het DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
0.109 2.24E-10 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.107 5.13E-10 YMR118C_p SHH3_p hom Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase
0.097 1.74E-8 YJL050W MTR4 het ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.093 7.61E-8 YJL136W-A_p YJL136W-A_p hom Putative protein of unknown function; identified by SAGE
0.088 3.54E-7 YDL229W SSB1 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
0.088 3.54E-7 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.086 7.02E-7 YGR173W RBG2 hom Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
0.085 9.44E-7 YKL187C FAT3 hom Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
0.085 9.32E-7 YMR176W ECM5 hom Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
0.084 9.66E-7 YHR091C MSR1 hom Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6