HIPHOP chemogenomics database

YLR034C / SMF3

Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR034C / SMF3

Click on Significant Values for Screen Details

Top fitness defect scores for YLR034C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2327	6189656	6.10	200.0 µM	4.79E-10		1	5.28E-10
2	SGTC_959	1222-0044	5.52	107.0 µM	1.53E-8		4	1.67E-8
3	SGTC_1120	ficusin	5.27	1.8 µM	6.16E-8		5	6.67E-8
4	SGTC_2415	st077333	5.16	57.7 µM	1.14E-7		17	1.24E-7
5	SGTC_1704	st028805	5.02	70.8 µM	2.44E-7		2	2.62E-7
6	SGTC_2013	4024628	4.66	200.0 µM	1.50E-6		28	1.61E-6
7	SGTC_2949	9072542	4.43	2.2 µM	4.40E-6	TSC3-RPN4	26	4.69E-6
8	SGTC_3299	9121982	4.09	43.1 µM	2.06E-5		23	2.18E-5
9	SGTC_258	haloperidol	4.06	50.8 µM	2.28E-5	fatty acid desaturase (OLE1)	28	2.42E-5
10	SGTC_1066	ketoconazole	4.03	628.0 nM	2.68E-5		19	2.84E-5
11	SGTC_2021	4019055	4.01	173.0 µM	2.84E-5	endomembrane recycling	119	3.00E-5
12	SGTC_1060	3448-7350	4.00	82.0 µM	3.00E-5	Golgi	33	3.17E-5
13	SGTC_1344	1488-0097	3.98	29.1 µM	3.24E-5		15	3.42E-5
14	SGTC_45	0kpi-0036	3.98	14.9 µM	3.32E-5	excess fatty acid	24	3.51E-5
15	SGTC_2634	4'-methoxyflavone	3.88	100.0 µM	4.88E-5		35	5.15E-5
16	SGTC_2395	5646831	3.78	180.2 µM	7.43E-5		27	7.83E-5
17	SGTC_2089	5281248	3.75	129.7 µM	8.38E-5	superoxide	27	8.82E-5
18	SGTC_247	mechlorethamine	3.73	29.7 µM	9.08E-5	DNA damage response	17	9.55E-5
19	SGTC_1287	0971-0001	3.70	34.6 µM	1.01E-4	sphingolipid biosynthesis & PDR1	52	1.07E-4
20	SGTC_2282	7944268	3.57	200.0 µM	1.72E-4	RPP1 & pyrimidine depletion	48	1.81E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.266	2.31E-55	YHL045W_d	YHL045W_d	hom	Putative protein of unknown function; not an essential gene
0.235	3.55E-43	YGL090W	LIF1	hom	Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.213	7.52E-36	YDL155W	CLB3	hom	B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.200	1.82E-31	YPL246C	RBD2	hom	Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.198	6.48E-31	YML029W	USA1	hom	Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.197	1.10E-30	YNL123W	NMA111	hom	Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.196	2.45E-30	YIL009W	FAA3	hom	Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.194	6.53E-30	YLR040C_p	YLR040C_p	hom	Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.186	1.91E-27	YDL022W	GPD1	hom	NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.185	2.45E-27	YLR440C	SEC39	het	Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
0.172	1.12E-23	YML046W	PRP39	het	U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.171	1.66E-23	YCR015C_p	YCR015C_p	hom	Putative protein of unknown function; YCR015C is not an essential gene
0.170	3.69E-23	YOR014W	RTS1	hom	B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
0.169	7.46E-23	YLR317W_d	YLR317W_d	het	Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.166	3.55E-22	YOR271C_p	FSF1_p	hom	Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis