YLR034C / SMF3

Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR034C / SMF3

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:-9.66|P-value:2.15E-22 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.98|P-value:3.32E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.90|P-value:4.95E-5 ID:SGTC_211|Compound:3455-0679|FD-Score:3.30|P-value:4.71E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-3.42|P-value:3.22E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.73|P-value:9.08E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:4.06|P-value:2.28E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.14|P-value:8.64E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.41|P-value:3.05E-4 ID:SGTC_458|Compound:nppb|FD-Score:-3.18|P-value:7.37E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.37|P-value:3.54E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-7.22|P-value:2.64E-13 ID:SGTC_819|Compound:1013-0258|FD-Score:3.35|P-value:3.80E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.25|P-value:5.54E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:5.52|P-value:1.53E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.00|P-value:3.00E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.03|P-value:2.68E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:5.27|P-value:6.16E-8 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.70|P-value:1.01E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.98|P-value:3.24E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.18|P-value:7.39E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.19|P-value:6.76E-4 ID:SGTC_1700|Compound:st030840|FD-Score:-3.10|P-value:9.79E-4 ID:SGTC_1704|Compound:st028805|FD-Score:5.02|P-value:2.44E-7 ID:SGTC_1708|Compound:st033234|FD-Score:-4.01|P-value:3.02E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.10|P-value:9.24E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.14|P-value:8.13E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.17|P-value:7.42E-4 ID:SGTC_1963|Compound:st076565|FD-Score:-3.31|P-value:4.70E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.29|P-value:5.14E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.66|P-value:1.50E-6 ID:SGTC_2021|Compound:4019055|FD-Score:4.01|P-value:2.84E-5 ID:SGTC_2078|Compound:5212016|FD-Score:3.35|P-value:3.85E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.75|P-value:8.38E-5 ID:SGTC_2282|Compound:7944268|FD-Score:3.57|P-value:1.72E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-3.56|P-value:1.89E-4 ID:SGTC_2327|Compound:6189656|FD-Score:6.10|P-value:4.79E-10 ID:SGTC_2351|Compound:9026858|FD-Score:3.26|P-value:5.32E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.45|P-value:2.65E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.78|P-value:7.43E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.17|P-value:1.51E-5 ID:SGTC_2415|Compound:st077333|FD-Score:5.16|P-value:1.14E-7 ID:SGTC_2450|Compound:5739737|FD-Score:-3.45|P-value:2.83E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.47|P-value:2.53E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.88|P-value:4.88E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.43|P-value:2.91E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.43|P-value:4.40E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.09|P-value:2.06E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-9.66|P-value:2.15E-22 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.98|P-value:3.32E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.90|P-value:4.95E-5 ID:SGTC_211|Compound:3455-0679|FD-Score:3.30|P-value:4.71E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:-3.42|P-value:3.22E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.73|P-value:9.08E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:4.06|P-value:2.28E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.14|P-value:8.64E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.41|P-value:3.05E-4 ID:SGTC_458|Compound:nppb|FD-Score:-3.18|P-value:7.37E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.37|P-value:3.54E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-7.22|P-value:2.64E-13 ID:SGTC_819|Compound:1013-0258|FD-Score:3.35|P-value:3.80E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.25|P-value:5.54E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:5.52|P-value:1.53E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.00|P-value:3.00E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.03|P-value:2.68E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:5.27|P-value:6.16E-8 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.70|P-value:1.01E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.98|P-value:3.24E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.18|P-value:7.39E-4 ID:SGTC_1659|Compound:st012954|FD-Score:3.19|P-value:6.76E-4 ID:SGTC_1700|Compound:st030840|FD-Score:-3.10|P-value:9.79E-4 ID:SGTC_1704|Compound:st028805|FD-Score:5.02|P-value:2.44E-7 ID:SGTC_1708|Compound:st033234|FD-Score:-4.01|P-value:3.02E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.10|P-value:9.24E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.14|P-value:8.13E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.17|P-value:7.42E-4 ID:SGTC_1963|Compound:st076565|FD-Score:-3.31|P-value:4.70E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.29|P-value:5.14E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.66|P-value:1.50E-6 ID:SGTC_2021|Compound:4019055|FD-Score:4.01|P-value:2.84E-5 ID:SGTC_2078|Compound:5212016|FD-Score:3.35|P-value:3.85E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.75|P-value:8.38E-5 ID:SGTC_2282|Compound:7944268|FD-Score:3.57|P-value:1.72E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-3.56|P-value:1.89E-4 ID:SGTC_2327|Compound:6189656|FD-Score:6.10|P-value:4.79E-10 ID:SGTC_2351|Compound:9026858|FD-Score:3.26|P-value:5.32E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.45|P-value:2.65E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.78|P-value:7.43E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.17|P-value:1.51E-5 ID:SGTC_2415|Compound:st077333|FD-Score:5.16|P-value:1.14E-7 ID:SGTC_2450|Compound:5739737|FD-Score:-3.45|P-value:2.83E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.47|P-value:2.53E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.88|P-value:4.88E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.43|P-value:2.91E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.43|P-value:4.40E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.09|P-value:2.06E-5

Top fitness defect scores for YLR034C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2327 6189656 6.10 200.0 µM 4.79E-10 1 5.28E-10
2 SGTC_959 1222-0044 5.52 107.0 µM 1.53E-8 4 1.67E-8
3 SGTC_1120 ficusin 5.27 1.8 µM 6.16E-8 5 6.67E-8
4 SGTC_2415 st077333 5.16 57.7 µM 1.14E-7 17 1.24E-7
5 SGTC_1704 st028805 5.02 70.8 µM 2.44E-7 2 2.62E-7
6 SGTC_2013 4024628 4.66 200.0 µM 1.50E-6 28 1.61E-6
7 SGTC_2949 9072542 4.43 2.2 µM 4.40E-6 TSC3-RPN4 26 4.69E-6
8 SGTC_3299 9121982 4.09 43.1 µM 2.06E-5 23 2.18E-5
9 SGTC_258 haloperidol 4.06 50.8 µM 2.28E-5 fatty acid desaturase (OLE1) 28 2.42E-5
10 SGTC_1066 ketoconazole 4.03 628.0 nM 2.68E-5 19 2.84E-5
11 SGTC_2021 4019055 4.01 173.0 µM 2.84E-5 endomembrane recycling 119 3.00E-5
12 SGTC_1060 3448-7350 4.00 82.0 µM 3.00E-5 Golgi 33 3.17E-5
13 SGTC_1344 1488-0097 3.98 29.1 µM 3.24E-5 15 3.42E-5
14 SGTC_45 0kpi-0036 3.98 14.9 µM 3.32E-5 excess fatty acid 24 3.51E-5
15 SGTC_2634 4'-methoxyflavone 3.88 100.0 µM 4.88E-5 35 5.15E-5
16 SGTC_2395 5646831 3.78 180.2 µM 7.43E-5 27 7.83E-5
17 SGTC_2089 5281248 3.75 129.7 µM 8.38E-5 superoxide 27 8.82E-5
18 SGTC_247 mechlorethamine 3.73 29.7 µM 9.08E-5 DNA damage response 17 9.55E-5
19 SGTC_1287 0971-0001 3.70 34.6 µM 1.01E-4 sphingolipid biosynthesis & PDR1 52 1.07E-4
20 SGTC_2282 7944268 3.57 200.0 µM 1.72E-4 RPP1 & pyrimidine depletion 48 1.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.266 2.31E-55 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.235 3.55E-43 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.213 7.52E-36 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.200 1.82E-31 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.198 6.48E-31 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.197 1.10E-30 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.196 2.45E-30 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.194 6.53E-30 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.186 1.91E-27 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.185 2.45E-27 YLR440C SEC39 het Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
0.172 1.12E-23 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.171 1.66E-23 YCR015C_p YCR015C_p hom Putative protein of unknown function; YCR015C is not an essential gene
0.170 3.69E-23 YOR014W RTS1 hom B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
0.169 7.46E-23 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.166 3.55E-22 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis