HIPHOP chemogenomics database

YLR058C / SHM2

Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR058C / SHM2

Click on Significant Values for Screen Details

Top fitness defect scores for YLR058C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_123	0083-0120	14.60	66.7 µM	1.27E-50		6	2.79E-48
2	SGTC_3244	9134489	9.23	49.5 µM	1.55E-21		5	1.36E-20
3	SGTC_2679	rapamycin	7.69	1.0 nM	1.68E-15		44	7.61E-15
4	SGTC_832	4083-0019	5.83	164.0 µM	1.13E-9	translation	23	2.71E-9
5	SGTC_1467	k284-1082	5.44	293.0 µM	1.23E-8		5	2.64E-8
6	SGTC_831	k061-1017	5.24	217.0 µM	3.86E-8	translation	18	7.86E-8
7	SGTC_2440	5628896	4.55	157.8 µM	1.53E-6		16	2.63E-6
8	SGTC_830	4076-0286	4.55	57.8 µM	1.57E-6	translation	40	2.68E-6
9	SGTC_498	2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)	4.25	150.0 µM	6.82E-6	Golgi	38	1.09E-5
10	SGTC_920	mycophenolic acid	4.20	780.4 nM	8.59E-6		22	1.36E-5
11	SGTC_309	k408-0051	4.14	1.5 µM	1.12E-5		42	1.75E-5
12	SGTC_1844	rapamycin	4.08	4.0 nM	1.45E-5		152	2.25E-5
13	SGTC_2442	5158906	4.07	48.5 µM	1.55E-5		19	2.40E-5
14	SGTC_510	ag-490	4.06	170.0 µM	1.56E-5		2	2.41E-5
15	SGTC_2572	astragaloside iv	4.06	100.0 µM	1.61E-5		22	2.47E-5
16	SGTC_2059	5238311	4.01	157.0 µM	1.95E-5		25	2.97E-5
17	SGTC_1228	0124-0032	3.86	52.5 µM	3.88E-5		18	5.74E-5
18	SGTC_508	roscovitine	3.80	141.0 µM	5.04E-5		8	7.37E-5
19	SGTC_364	0083-0097	3.76	67.7 µM	5.97E-5		29	8.66E-5
20	SGTC_611	3453-1652	3.69	23.7 µM	7.74E-5		30	1.11E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.468	1.72E-182	YGR204W	ADE3	hom	Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.371	3.49E-110	YGL234W	ADE5,7	hom	Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.317	4.93E-79	YGR061C	ADE6	hom	Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.279	4.93E-61	YMR300C	ADE4	hom	Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.277	3.40E-60	YDR408C	ADE8	hom	Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.193	1.72E-29	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.178	3.66E-25	YKL110C	KTI12	hom	Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.166	3.42E-22	YLR048W	RPS0B	hom	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.160	1.18E-20	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.158	4.19E-20	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.157	6.07E-20	YHR187W	IKI1	hom	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.148	6.03E-18	YMR120C	ADE17	hom	Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.146	1.48E-17	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.146	1.87E-17	YPL235W	RVB2	het	ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
0.142	1.12E-16	YHR010W	RPL27A	hom	Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication