YLR058C / SHM2

Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR058C / SHM2

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:3.10|P-value:7.40E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:14.60|P-value:1.27E-50 ID:SGTC_309|Compound:k408-0051|FD-Score:4.14|P-value:1.12E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.19|P-value:5.35E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.76|P-value:5.97E-5 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.25|P-value:6.82E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:3.26|P-value:4.21E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.80|P-value:5.04E-5 ID:SGTC_510|Compound:ag-490|FD-Score:4.06|P-value:1.56E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.69|P-value:7.74E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.46|P-value:1.98E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:-4.60|P-value:1.19E-6 ID:SGTC_729|Compound:4092-0585|FD-Score:3.07|P-value:8.16E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.42|P-value:2.28E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.20|P-value:5.20E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:4.55|P-value:1.57E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:5.24|P-value:3.86E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:5.83|P-value:1.13E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.20|P-value:8.59E-6 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.12|P-value:6.94E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.56|P-value:1.33E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.22|P-value:4.79E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.86|P-value:3.88E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.05|P-value:8.62E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:5.44|P-value:1.23E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-4.29|P-value:5.43E-6 ID:SGTC_1745|Compound:st081495|FD-Score:3.06|P-value:8.63E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-4.26|P-value:6.14E-6 ID:SGTC_1768|Compound:st044301|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.08|P-value:1.45E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.10|P-value:7.26E-4 ID:SGTC_2059|Compound:5238311|FD-Score:4.01|P-value:1.95E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_2376|Compound:9072922|FD-Score:-3.93|P-value:2.73E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.31|P-value:3.51E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:-3.15|P-value:6.11E-4 ID:SGTC_2440|Compound:5628896|FD-Score:4.55|P-value:1.53E-6 ID:SGTC_2442|Compound:5158906|FD-Score:4.07|P-value:1.55E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.06|P-value:1.61E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.03|P-value:9.35E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.69|P-value:1.68E-15 ID:SGTC_2825|Compound:7998094|FD-Score:-3.11|P-value:7.06E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.59|P-value:1.18E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.62|P-value:1.00E-4 ID:SGTC_3244|Compound:9134489|FD-Score:9.23|P-value:1.55E-21 ID:SGTC_3284|Compound:9108171|FD-Score:-3.54|P-value:1.40E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.18|P-value:5.54E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.10|P-value:7.40E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:14.60|P-value:1.27E-50 ID:SGTC_309|Compound:k408-0051|FD-Score:4.14|P-value:1.12E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.19|P-value:5.35E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.76|P-value:5.97E-5 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.25|P-value:6.82E-6 ID:SGTC_504|Compound:lovastatin|FD-Score:3.26|P-value:4.21E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.80|P-value:5.04E-5 ID:SGTC_510|Compound:ag-490|FD-Score:4.06|P-value:1.56E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.69|P-value:7.74E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:3.46|P-value:1.98E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:-4.60|P-value:1.19E-6 ID:SGTC_729|Compound:4092-0585|FD-Score:3.07|P-value:8.16E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.42|P-value:2.28E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.20|P-value:5.20E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:4.55|P-value:1.57E-6 ID:SGTC_831|Compound:k061-1017|FD-Score:5.24|P-value:3.86E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:5.83|P-value:1.13E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.20|P-value:8.59E-6 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.12|P-value:6.94E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.56|P-value:1.33E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.22|P-value:4.79E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.86|P-value:3.88E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.05|P-value:8.62E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:5.44|P-value:1.23E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_1744|Compound:st037937|FD-Score:-4.29|P-value:5.43E-6 ID:SGTC_1745|Compound:st081495|FD-Score:3.06|P-value:8.63E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-4.26|P-value:6.14E-6 ID:SGTC_1768|Compound:st044301|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.08|P-value:1.45E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.10|P-value:7.26E-4 ID:SGTC_2059|Compound:5238311|FD-Score:4.01|P-value:1.95E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.27|P-value:4.06E-4 ID:SGTC_2376|Compound:9072922|FD-Score:-3.93|P-value:2.73E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.31|P-value:3.51E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:-3.15|P-value:6.11E-4 ID:SGTC_2440|Compound:5628896|FD-Score:4.55|P-value:1.53E-6 ID:SGTC_2442|Compound:5158906|FD-Score:4.07|P-value:1.55E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:4.06|P-value:1.61E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-3.03|P-value:9.35E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.69|P-value:1.68E-15 ID:SGTC_2825|Compound:7998094|FD-Score:-3.11|P-value:7.06E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.59|P-value:1.18E-4 ID:SGTC_3068|Compound:9116768|FD-Score:-3.62|P-value:1.00E-4 ID:SGTC_3244|Compound:9134489|FD-Score:9.23|P-value:1.55E-21 ID:SGTC_3284|Compound:9108171|FD-Score:-3.54|P-value:1.40E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.18|P-value:5.54E-4

Top fitness defect scores for YLR058C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_123 0083-0120 14.60 66.7 µM 1.27E-50 6 2.79E-48
2 SGTC_3244 9134489 9.23 49.5 µM 1.55E-21 5 1.36E-20
3 SGTC_2679 rapamycin 7.69 1.0 nM 1.68E-15 44 7.61E-15
4 SGTC_832 4083-0019 5.83 164.0 µM 1.13E-9 translation 23 2.71E-9
5 SGTC_1467 k284-1082 5.44 293.0 µM 1.23E-8 5 2.64E-8
6 SGTC_831 k061-1017 5.24 217.0 µM 3.86E-8 translation 18 7.86E-8
7 SGTC_2440 5628896 4.55 157.8 µM 1.53E-6 16 2.63E-6
8 SGTC_830 4076-0286 4.55 57.8 µM 1.57E-6 translation 40 2.68E-6
9 SGTC_498 2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 4.25 150.0 µM 6.82E-6 Golgi 38 1.09E-5
10 SGTC_920 mycophenolic acid 4.20 780.4 nM 8.59E-6 22 1.36E-5
11 SGTC_309 k408-0051 4.14 1.5 µM 1.12E-5 42 1.75E-5
12 SGTC_1844 rapamycin 4.08 4.0 nM 1.45E-5 152 2.25E-5
13 SGTC_2442 5158906 4.07 48.5 µM 1.55E-5 19 2.40E-5
14 SGTC_510 ag-490 4.06 170.0 µM 1.56E-5 2 2.41E-5
15 SGTC_2572 astragaloside iv 4.06 100.0 µM 1.61E-5 22 2.47E-5
16 SGTC_2059 5238311 4.01 157.0 µM 1.95E-5 25 2.97E-5
17 SGTC_1228 0124-0032 3.86 52.5 µM 3.88E-5 18 5.74E-5
18 SGTC_508 roscovitine 3.80 141.0 µM 5.04E-5 8 7.37E-5
19 SGTC_364 0083-0097 3.76 67.7 µM 5.97E-5 29 8.66E-5
20 SGTC_611 3453-1652 3.69 23.7 µM 7.74E-5 30 1.11E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.468 1.72E-182 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.371 3.49E-110 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.317 4.93E-79 YGR061C ADE6 hom Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.279 4.93E-61 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.277 3.40E-60 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.193 1.72E-29 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.178 3.66E-25 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.166 3.42E-22 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.160 1.18E-20 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.158 4.19E-20 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.157 6.07E-20 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.148 6.03E-18 YMR120C ADE17 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.146 1.48E-17 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.146 1.87E-17 YPL235W RVB2 het ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
0.142 1.12E-16 YHR010W RPL27A hom Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication