HIPHOP chemogenomics database
YLR100W / ERG27 
3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YLR100W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.223 | 3.24E-39 | YLR101C_d | YLR101C_d | het | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W |
| 0.152 | 8.51E-19 | YOL050C_d | YOL050C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) |
| 0.119 | 4.68E-12 | YJR005W | APL1 | hom | Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex |
| 0.118 | 6.22E-12 | YHR021W-A_p | ECM12_p | hom | Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity |
| 0.108 | 3.78E-10 | YGR245C | SDA1 | het | Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis |
| 0.100 | 6.82E-9 | YGL013C | PDR1 | hom | Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication |
| 0.086 | 7.01E-7 | YDR468C | TLG1 | het | Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p |
| 0.084 | 9.53E-7 | YNR054C | ESF2 | het | Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome |
| 0.084 | 1.09E-6 | YOR291W | YPK9 | hom | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome |
| 0.083 | 1.67E-6 | YJL061W | NUP82 | het | Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) |
| 0.082 | 2.12E-6 | YGR264C | MES1 | het | Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs |
| 0.080 | 3.03E-6 | YOR021C | SFM1 | hom | SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism |
| 0.079 | 4.14E-6 | YMR034C_p | YMR034C_p | hom | Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene |
| 0.079 | 5.20E-6 | YJL194W | CDC6 | het | Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| 0.077 | 7.47E-6 | YJL032W_d | YJL032W_d | het | Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase |